公开(公告)号：US5223220A

公开(公告)日：1993-06-29

申请号：US917800

申请日：1992-07-20

申请人： Eugene Fan ,  Sinfu Tzeng ,  Fon-Chiu M. Chen ,  Ching Huang ,  Dou-Mei Wang ,  Theresa Popejoy

发明人： Eugene Fan ,  Sinfu Tzeng ,  Fon-Chiu M. Chen ,  Ching Huang ,  Dou-Mei Wang ,  Theresa Popejoy

IPC分类号： B01L3/00 ,  G01N33/53 ,  G01N33/543 ,  G01N37/00

CPC分类号： B01L3/5023 ,  G01N33/5302 ,  G01N33/54366 ,  B01L2300/0663 ,  B01L2300/0809 ,  B01L2300/105 ,  Y10S435/805

摘要： In response to an express need for an immunoassay device with universal applicability, which further promotes the goals of inventory reduction and simplified, less costly manufacture, a container for holding materials used in conjunction with an immunoassay, having a housing with an inside, an outside, and a top, wherein the top is provided with a plurality of apertures communicating with the inside of the housing, and a thin web of opaque material applied over the top of the housing covering at least one but not all of the apertures.

摘要翻译： 响应于对具有通用适用性的免疫测定装置的明确需要，其进一步促进库存减少和简化，较便宜的制造的目标，用于保持与免疫测定结合使用的材料的容器，具有带有内部的外壳，外部 和顶部，其中所述顶部设置有与所述壳体的内部连通的多个孔，以及施加在所述壳体的顶部上的薄的不透明材料幅材，其覆盖至少一个但不是全部的所述孔。

公开(公告)号：US5096837A

公开(公告)日：1992-03-17

申请号：US476788

申请日：1990-02-08

申请人： Eugene Fan ,  Dou-Mei Wang ,  Fon-Chiu M. Chen ,  Gregg L. Wilson ,  Michael W. Milner ,  Ching Huang

发明人： Eugene Fan ,  Dou-Mei Wang ,  Fon-Chiu M. Chen ,  Gregg L. Wilson ,  Michael W. Milner ,  Ching Huang

IPC分类号： G01N33/543 ,  G01N33/558

CPC分类号： G01N33/54313 ,  G01N33/558 ,  Y10S435/962 ,  Y10S436/814

摘要： The invention disclosed herein relates to the field of one-step assays and latex agglutination tests ("LAT"), and provides an improved method of preparing and using a one-step immunoassay, as well as improved methods of preparing coated latex particles for use in diagnostic assays, and especially, immunochromatographic assays.

摘要翻译： 本文公开的本发明涉及一步测定和乳胶凝集试验（“LAT”）的领域，并且提供了制备和使用一步免疫测定法的改进方法，以及制备用于使用的涂覆胶乳颗粒的改进方法 在诊断测定中，特别是免疫色谱测定。

公开(公告)号：USD324426S

公开(公告)日：1992-03-03

申请号：US424588

申请日：1989-10-20

申请人： Eugene Fan ,  Ching Huang ,  Dou-Mei Wang ,  Gregg L. Wilson

设计人： Eugene Fan ,  Ching Huang ,  Dou-Mei Wang ,  Gregg L. Wilson

公开(公告)号：USD328135S

公开(公告)日：1992-07-21

申请号：US463981

申请日：1990-01-12

申请人： Eugene Fan ,  Ching Huang ,  Dou-Mei Wang ,  Gregg L. Wilson

设计人： Eugene Fan ,  Ching Huang ,  Dou-Mei Wang ,  Gregg L. Wilson

公开(公告)号：US06194221B1

公开(公告)日：2001-02-27

申请号：US08963412

申请日：1997-11-03

申请人： Leslie Rehg ,  Ching Huang ,  Michael J. Willrodt ,  Herbert Bradfield Cunningham ,  Eugene Fan

发明人： Leslie Rehg ,  Ching Huang ,  Michael J. Willrodt ,  Herbert Bradfield Cunningham ,  Eugene Fan

IPC分类号： G01N33533

CPC分类号： G01N33/54386 ,  G01N30/02 ,  G01N30/90 ,  G01N33/558 ,  Y10S435/885 ,  Y10S435/969 ,  Y10S435/97 ,  B01D15/3804

摘要： This invention relates to a lateral flow immunochromatographic assay device with an increased range of sensitivity without an increase in the clearance time or the occurrence of false positive results. The indicator reagent for the analyte is located in both a separate labeling reagent region and a discrete zone of the analyte detection region.

摘要翻译： 本发明涉及一种具有增加的灵敏度范围而不增加清除时间或产生假阳性结果的侧流免疫层析测定装置。 分析物的指示剂位于分析物检测区域的分离的标记试剂区域和离散区域中。

公开(公告)号：US20050277163A1

公开(公告)日：2005-12-15

申请号：US11154274

申请日：2005-06-15

申请人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

发明人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

IPC分类号： G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  G01N33/554 ,  G01N33/558 ,  G01N33/569

CPC分类号： G01N33/558 ,  G01N33/56944 ,  Y10S436/807 ,  Y10S436/81 ,  Y10T436/25 ,  Y10T436/25375

摘要： A method to determine the presence or absence of Streptococcus Group A antigen in a sample, comprising the following steps: extracting the antigen from the sample in an assay chamber with two or less extraction reagents, wherein the two reagents may be added to the assay chamber in no particular sequence; introducing a lateral flow immunochromatographic assay device into the extraction reagents containing the extracted antigen without further addition of reagents or manipulation of the sample; forming an antigen-indicator labeling reagent complex; and determining the presence or absence of the antigen in the sample by the presence or absence of a signal formed by the binding of the antigen-indicator labeling reagent complex to an indicator capture reagent specific for said antigen-indicator labeling reagent complex.

摘要翻译： 一种确定样品中A组抗原抗原存在或不存在的方法，包括以下步骤：在具有两种或更少种提取试剂的测定室中从样品中提取抗原，其中两种试剂可以加入到测定室 没有特别的顺序; 在含有提取的抗原的提取试剂中引入侧流免疫层析测定装置，而无需进一步添加试剂或操作样品; 形成抗原指示标记试剂复合物; 通过存在或不存在通过将抗原指示标记试剂复合物与特异于所述抗原指示标记试剂复合物的指示剂捕获试剂的结合而形成的信号来确定样品中抗原的存在或不存在。

公开(公告)号：US20060154315A1

公开(公告)日：2006-07-13

申请号：US11366761

申请日：2006-03-02

申请人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

发明人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

IPC分类号： G01N33/554 ,  G01N33/569

CPC分类号： G01N33/558 ,  G01N33/56944 ,  Y10S436/807 ,  Y10S436/81 ,  Y10T436/25 ,  Y10T436/25375

摘要： A method to determine the presence or absence of Streptococcus Group A antigen in a sample, comprising the following steps: extracting the antigen from the sample in an assay chamber with two or less extraction reagents, wherein the two reagents may be added to the assay chamber in no particular sequence; introducing a lateral flow immunochromatographic assay device into the extraction reagents containing the extracted antigen without further addition of reagents or manipulation of the sample; forming an antigen-indicator labeling reagent complex; and determining the presence or absence of the antigen in the sample by the presence or absence of a signal formed by the binding of the antigen-indicator labeling reagent complex to an indicator capture reagent specific for said antigen-indicator labeling reagent complex.

摘要翻译： 一种确定样品中A组抗原抗原存在或不存在的方法，包括以下步骤：在具有两个或更少提取试剂的测定室中从样品中提取抗原，其中两种试剂可以加入到测定室 没有特别的顺序; 在含有提取的抗原的提取试剂中引入侧流免疫层析测定装置，而无需进一步添加试剂或操作样品; 形成抗原指示标记试剂复合物; 通过存在或不存在通过将抗原指示标记试剂复合物与特异于所述抗原指示标记试剂复合物的指示剂捕获试剂的结合而形成的信号来确定样品中抗原的存在或不存在。

公开(公告)号：US20070292902A1

公开(公告)日：2007-12-20

申请号：US11880311

申请日：2007-07-20

申请人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

发明人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael Willrodt ,  Herbert Cunningham ,  Eugene Fan

IPC分类号： G01N33/569

CPC分类号： G01N33/558 ,  G01N33/56944 ,  Y10S436/807 ,  Y10S436/81 ,  Y10T436/25 ,  Y10T436/25375

摘要： A method to determine the presence or absence of Streptococcus Group A antigen in a sample, comprising the following steps: extracting the antigen from the sample in an assay chamber with two or less extraction reagents, wherein the two reagents may be added to the assay chamber in no particular sequence; introducing a lateral flow immunochromatographic assay device into the extraction reagents containing the extracted antigen without further addition of reagents or manipulation of the sample; forming an antigen-indicator labeling reagent complex; and determining the presence or absence of the antigen in the sample by the presence or absence of a signal formed by the binding of the antigen-indicator labeling reagent complex to an indicator capture reagent specific for said antigen-indicator labeling reagent complex.

摘要翻译： 一种确定样品中A组抗原抗原存在或不存在的方法，包括以下步骤：在具有两种或更少种提取试剂的测定室中从样品中提取抗原，其中两种试剂可以加入到测定室 没有特别的顺序; 在含有提取的抗原的提取试剂中引入侧流免疫层析测定装置，而无需进一步添加试剂或操作样品; 形成抗原指示标记试剂复合物; 通过存在或不存在通过将抗原指示标记试剂复合物与特异于所述抗原指示标记试剂复合物的指示剂捕获试剂的结合而形成的信号来确定样品中抗原的存在或不存在。

公开(公告)号：US07666614B2

公开(公告)日：2010-02-23

申请号：US11366761

申请日：2006-03-02

申请人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael J. Willrodt ,  Herbert Bradfield Cunningham ,  Eugene Fan

发明人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael J. Willrodt ,  Herbert Bradfield Cunningham ,  Eugene Fan

IPC分类号： C12Q1/00 ,  G01N33/53 ,  G01N33/567 ,  G01N33/00 ,  C12M1/00

CPC分类号： G01N33/558 ,  G01N33/56944 ,  Y10S436/807 ,  Y10S436/81 ,  Y10T436/25 ,  Y10T436/25375

摘要： A method to determine the presence or absence of Streptococcus Group A antigen in a sample, comprising the following steps: extracting the antigen from the sample in an assay chamber with two or less extraction reagents, wherein the two reagents may be added to the assay chamber in no particular sequence; introducing a lateral flow immunochromatographic assay device into the extraction reagents containing the extracted antigen without further addition of reagents or manipulation of the sample; forming an antigen-indicator labeling reagent complex; and determining the presence or absence of the antigen in the sample by the presence or absence of a signal formed by the binding of the antigen-indicator labeling reagent complex to an indicator capture reagent specific for said antigen-indicator labeling reagent complex.

摘要翻译： 一种确定样品中A组抗原抗原存在或不存在的方法，包括以下步骤：在具有两种或更少种提取试剂的测定室中从样品中提取抗原，其中两种试剂可以加入到测定室 没有特别的顺序; 在含有提取的抗原的提取试剂中引入侧流免疫层析测定装置，而无需进一步添加试剂或操作样品; 形成抗原指示标记试剂复合物; 通过存在或不存在通过将抗原指示标记试剂复合物与特异于所述抗原指示标记试剂复合物的指示剂捕获试剂的结合而形成的信号来确定样品中抗原的存在或不存在。

公开(公告)号：US06979576B1

公开(公告)日：2005-12-27

申请号：US08900559

申请日：1997-07-25

申请人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael J. Willrodt ,  Herbert Bradfield Cunningham ,  Eugene Fan

发明人： Shu-Ching Cheng ,  Ching Huang ,  Ming-Shian Wu ,  Michael J. Willrodt ,  Herbert Bradfield Cunningham ,  Eugene Fan

IPC分类号： G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  G01N33/554 ,  G01N33/558 ,  G01N33/569

CPC分类号： G01N33/558 ,  G01N33/56944 ,  Y10S436/807 ,  Y10S436/81 ,  Y10T436/25 ,  Y10T436/25375

摘要： A method to determine the presence or absence of Streptococcus Group A antigen in a sample, comprising the following steps: extracting the antigen from the sample in an assay chamber with two or less extraction reagents, wherein the two reagents may be added to the assay chamber in no particular sequence; introducing a lateral flow immunochromatographic assay device into the extraction reagents containing the extracted antigen without further addition of reagents or manipulation of the sample; forming an antigen-indicator labeling reagent complex; and determining the presence or absence of the antigen in the sample by the presence or absence of a signal formed by the binding of the antigen-indicator labeling reagent complex to an indicator capture reagent specific for said antigen-indicator labeling reagent complex.

摘要翻译： 一种确定样品中A组抗原抗原存在或不存在的方法，包括以下步骤：在具有两种或更少种提取试剂的测定室中从样品中提取抗原，其中两种试剂可以加入到测定室 没有特别的顺序; 在含有提取的抗原的提取试剂中引入侧流免疫层析测定装置，而无需进一步添加试剂或操作样品; 形成抗原指示标记试剂复合物; 通过存在或不存在通过将抗原指示标记试剂复合物与特异于所述抗原指示标记试剂复合物的指示剂捕获试剂的结合而形成的信号来确定样品中抗原的存在或不存在。