Methods of producing validamycin A analogs and uses thereof
    3.
    发明授权
    Methods of producing validamycin A analogs and uses thereof 有权
    生产有效霉素A类似物的方法及其用途

    公开(公告)号:US08101732B2

    公开(公告)日:2012-01-24

    申请号:US12560283

    申请日:2009-09-15

    IPC分类号: C07H15/00

    摘要: This disclosure relates to validamycin A biosynthesis and in particular, to methods of producing validamycin A analogs and uses thereof. In a particular example, a method for making a validamycin A analog includes transforming a host cell with one or more recombinant DNA vectors to produce a valN-inactivated mutant; and culturing the valN-inactivated mutant in a culture medium to produce a validamycin A analog, such as 1,1′-bis-valienamine and validienamycin, and their conversion to valienamine. The present disclosure further relates to compositions including such compounds as well as methods of using the compositions, such as for antifungal agents.

    摘要翻译: 本公开涉及有效霉素A生物合成,特别涉及产生有效霉素A类似物的方法及其用途。 在具体实例中,制备有效霉素A类似物的方法包括用一种或多种重组DNA载体转化宿主细胞以产生ValN失活的突变体; 并在培养基中培养valN灭活的突变体以产生有效霉素A类似物,例如1,1'-双 - 缬胺和有效霉素,以及它们转化为valienamine。 本公开还涉及包含这些化合物的组合物以及使用组合物如抗真菌剂的方法。

    METHODS OF PRODUCING VALIDAMYCIN A ANALOGS AND USES THEREOF
    4.
    发明申请
    METHODS OF PRODUCING VALIDAMYCIN A ANALOGS AND USES THEREOF 有权
    生产喹哪啶霉素类似物的方法及其用途

    公开(公告)号:US20100151528A1

    公开(公告)日:2010-06-17

    申请号:US12560283

    申请日:2009-09-15

    摘要: This disclosure relates to validamycin A biosynthesis and in particular, to methods of producing validamycin A analogs and uses thereof. In a particular example, a method for making a validamycin A analog includes transforming a host cell with one or more recombinant DNA vectors to produce a valN-inactivated mutant; and culturing the valN-inactivated mutant in a culture medium to produce a validamycin A analog, such as 1,1′-bis-valienamine and validienamycin, and their conversion to valienamine. The present disclosure further relates to compositions including such compounds as well as methods of using the compositions, such as for antifungal agents.

    摘要翻译: 本公开涉及有效霉素A生物合成,特别涉及产生有效霉素A类似物的方法及其用途。 在具体实例中,制备有效霉素A类似物的方法包括用一种或多种重组DNA载体转化宿主细胞以产生ValN失活的突变体; 并在培养基中培养valN灭活的突变体以产生有效霉素A类似物,例如1,1'-双 - 缬胺和有效霉素,以及它们转化为valienamine。 本公开还涉及包含这些化合物的组合物以及使用组合物如抗真菌剂的方法。