公开(公告)号：US10745741B2

公开(公告)日：2020-08-18

申请号：US15579834

申请日：2016-06-27

申请人： EUROPEAN MOLECULAR BIOLOGY LABORATORY

发明人： Ramesh Utharala ,  Chawaree Chaipan ,  Lukas Nögel ,  Christoph A. Merten

IPC分类号： C12Q1/68 ,  B01L3/00 ,  C12Q1/6806 ,  B01L7/00

摘要： The present invention relates to the field of microfluidics and in particular to methods for co-localizing a particle comprising DNA and/or RNA with a known barcode oligonucleotide. Thereby, the transcriptome or DNA of a cell can be barcoded and correlated to a cell phenotype or examined for the effect of a drug on the cell. The invention also provides microfluidic devices and systems having properties which make them particularly suitable for use in the methods of the invention.

公开(公告)号：US12083512B2

公开(公告)日：2024-09-10

申请号：US16069824

申请日：2017-01-13

申请人： European Molecular Biology Laboratory

发明人： Hongxing Hu ,  Samantha Seah ,  Christoph A. Merten

IPC分类号： B01L3/00 ,  C07K16/28 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/686 ,  C40B20/08

CPC分类号： B01L3/502715 ,  B01L3/502761 ,  C07K16/28 ,  C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/686 ,  B01L2200/0647 ,  B01L2300/021 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/166 ,  C40B20/08 ,  C12Q1/6846 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

摘要： The present invention relates to the field of microfluidics and in particular to analysing the gene expression of a cell in response to a ligand expressed in the same microfluidic compartment. By barcoding the transcriptome of the cell and of the expression system generating the ligand, the effect of the ligand on the cell expression can be discerned. The invention provides microfluidic compartments and methods for this purpose.

公开(公告)号：US11524294B2

公开(公告)日：2022-12-13

申请号：US16334953

申请日：2017-09-20

申请人： European Molecular Biology Laboratory

发明人： Christoph A. Merten ,  Daniel Frenzel

IPC分类号： B01L3/00 ,  B03C5/02 ,  G01N15/14 ,  G01N15/00 ,  G01N15/10

摘要： The present invention relates to the field of microfluidics and in particular to devices and methods for sorting objects in microfluidic channels. These devices and methods allow for fast and robust sorting in two-way and multi-way setups. They also enable sorting over extended periods of time.

公开(公告)号：US10710078B2

公开(公告)日：2020-07-14

申请号：US15569201

申请日：2016-04-29

申请人： EUROPEAN MOLECULAR BIOLOGY LABORATORY

发明人： Christoph A. Merten ,  Hongxing Hu ,  David Eustace

IPC分类号： B01L3/00 ,  G01N15/14 ,  G01N33/50 ,  G01N33/58 ,  G01N33/48

摘要： The present invention relates to the field of microfluidics and in particular to methods for detecting microfluidic droplets and particles within droplets, as well as sorting the droplets. These methods allow for quantifying properties and activities of the particles within the droplets. For this purpose, the invention provides microfluidic droplets comprising suitably labelled particles. The invention also provides microfluidic devices and systems having properties which make them particularly suitable for use in the methods of the invention.