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公开(公告)号：US20210170394A1
公开(公告)日：2021-06-10
申请号：US17074009
申请日：2020-10-19
Applicant: FUJIFILM Corporation
Inventor： Takeshi HAMA , Noboru KOMORI , Yuki INOUE , Aya OUCHI
IPC: B01L3/00 , G01N33/543
Abstract: Provided is a liquid feeding device, including: a test container including a first accommodation portion, a second accommodation portion, and a third accommodation portion each accommodating a liquid, a first flow path connecting the first accommodation portion and the second accommodation portion to each other at respective upper end positions thereof, and a second flow path connecting the second accommodation portion and the third accommodation portion to each other at respective upper end positions thereof, in which at least a portion forming an upper wall surface of the second accommodation portion has flexibility; and a pressing machine which includes a first pressing portion and presses the portion forming the upper wall surface of the second accommodation portion at a position closer to the first flow path from a center in a liquid feeding direction towards an inside of the second accommodation portion from an outside by the pressing portion, in which the liquid accommodated in the second accommodation portion is fed to the third accommodation portion by pressing the portion forming the upper wall surface of the second accommodation portion with the pressing machine.
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公开(公告)号：US20210010606A1
公开(公告)日：2021-01-14
申请号：US17035220
申请日：2020-09-28
Applicant: FUJIFILM Corporation
Inventor： Yoshihisa USAMI , Yasuyuki ISHII , Yuki INOUE , Aya OUCHI
Abstract: A chip includes a first chamber that stores a fluid, a second chamber that stores a mixing target which is to be mixed with the fluid, a flow path that allows a communication between the first chamber and the second chamber, and a valve that is provided in the flow path and capable of changing the flow path by change in shape by heating.
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公开(公告)号：US20220168741A1
公开(公告)日：2022-06-02
申请号：US17670721
申请日：2022-02-14
Applicant: FUJIFILM Corporation
Inventor： Takeshi HAMA , Noboru KOMORI , Yuki INOUE , Aya OUCHI
IPC: B01L3/00 , C12Q1/6806 , G01N33/52
Abstract: In a container including two accommodation parts each accommodating liquid, mixing of the liquids in a case of moving magnetic particles in the liquid accommodated in one accommodation part to the other accommodation part is suppressed. Provided is a container includes a first accommodation part which accommodates a first liquid containing magnetic particles, a second accommodation part which accommodates separated magnetic particles separated from the first liquid, and a second liquid, and a flow passage which allows the first accommodation part and the second accommodation part to communicate with each other, and through which the separated magnetic particles pass, in which the flow passage has a first staircase part including two or more steps from an inner bottom surface of the first accommodation part on a first accommodation part side.
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公开(公告)号：US20210008569A1
公开(公告)日：2021-01-14
申请号：US17034040
申请日：2020-09-28
Applicant: FUJIFILM Corporation
Inventor： Yoshihisa USAMI , Yasuyuki ISHII , Yuki INOUE , Aya OUCHI
Abstract: A separation device includes: a container that includes a storage portion for storing liquid containing magnetic particles; and a coil that separates the magnetic particles from the liquid by generating a magnetic field through application of current, causing a magnetic force to act on the magnetic particles in a state where the coil stops with respect to the storage portion, and causing the magnetic particles to move from the storage portion against gravity acting on the liquid containing the magnetic particles.
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公开(公告)号：US20180087112A1
公开(公告)日：2018-03-29
申请号：US15712785
申请日：2017-09-22
Applicant: FUJIFILM Corporation
Inventor： Aya OUCHI , Takayuki TSUJIMOTO , Masaya NAGASE , Yasuyuki ISHII
IPC: C12Q1/6883 , C12N15/10
CPC classification number: C12Q1/6883 , C12N15/09 , C12N15/1089 , C12Q1/6811 , C12Q1/6876 , C12Q2600/156 , C12Q2600/16 , C12Q2535/122 , C12Q2537/165
Abstract: Provided is a method for determining a genetic condition of a fetus, the method including: an objective region selection step of selecting an objective region for determining the genetic condition, from regions on a human genome; a step of isolating a single cell from a maternal blood sample; a step of extracting genomic DNA from the single cell; a step of performing PCR amplification on the objective region using a primer set designed so as to perform the PCR amplification on the objective region using genomic DNA extracted from the single cell as a template; and a DNA sequencing step of decoding a DNA base sequence of a PCR amplification product of the objective region, in which the primer set designed so as to perform the PCR amplification on the objective region is designed through a method for designing a primer set used for the polymerase chain reaction, the method for designing a primer set including a first stage selection step based on a local alignment score and a second stage selection step based on a global alignment score.
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Patent Agency Ranking
公开(公告)号：US20170152555A1
公开(公告)日：2017-06-01
申请号：US15427050
申请日：2017-02-08
Applicant: FUJIFILM CORPORATION
Inventor： Yuki INOUE , Yasuyuki ISHII , Aya OUCHI
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12N15/09 , C12Q1/68 , C12Q1/6806
Abstract: A method for detecting the presence or absence of heteroploidy of fetal chromosomes includes: obtaining a primary amplification products by amplifying chromosomal DNA obtained from a biological sample collected from a pregnant mother; performing multiplex amplification of a plurality of target regions to obtain a secondary amplification product using the primary amplification product as a template; adding a label to both terminals of the secondary amplification product to obtain the labeled secondary amplification product; performing amplification using a primer pair annealing to the label using the labeled secondary amplification product as a template to obtain a tertiary amplification product; and determining the amplification amount and base sequences of the plurality of target regions from the tertiary amplification product. The primary amplification amplifies the total amount of DNA by 6000 to 30000 times, and the secondary amplification step amplifies the total amount of DNA by 3 to 150 times.
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公开(公告)号：US20210170404A1
公开(公告)日：2021-06-10
申请号：US16950434
申请日：2020-11-17
Applicant: FUJIFILM Corporation
Inventor： Takeshi HAMA , Noboru KOMORI , Yuki INOUE , Aya OUCHI
IPC: B01L3/00 , G01N33/543
Abstract: A test container including: at least two accommodation portions each accommodating a liquid and internally provided; a flow path connecting the two accommodation portions to each other at respective upper end positions thereof internally provided; and a flexible film deformable inwards of at least one accommodation portion at a portion forming an upper wall surface of the one accommodation portion, in which the liquid accommodated in the one accommodation portion is fed to the other accommodation portion via the flow path due to deformation of the flexible film towards the one accommodation portion, and a breaking elongation of the flexible film is 100% or more and 600% or less.
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公开(公告)号：US20210170392A1
公开(公告)日：2021-06-10
申请号：US16952198
申请日：2020-11-19
Applicant: FUJIFILM Corporation
Inventor： Takeshi HAMA , Noboru KOMORI , Yuki INOUE , Aya OUCHI
IPC: B01L3/00
Abstract: A test container includes a container main body including a first-accommodation-portion, a second-accommodation-portion, and a third-accommodation-portion each accommodating a liquid and internally provided, a first flow path connecting the first-accommodation-portion and the second-accommodation-portion to each other at respective upper end positions thereof and internally provided, and a second flow path connecting the second-accommodation-portion and the third-accommodation-portion to each other at respective upper end positions thereof and internally provided, in which at least a portion forming an upper wall surface of the second-accommodation-portion has flexibility to be deformable inwards of the second-accommodation-portion; and a liquid return prevention structure which prevents a backflow of the liquid to the first-accommodation-portion, when the liquid accommodated in the second-accommodation-portion is fed to the third-accommodation-portion via the second flow path due to deformation of the portion forming the upper wall surface of the second-accommodation-portion inwards of the second-accommodation-portion.
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公开(公告)号：US20210023565A1
公开(公告)日：2021-01-28
申请号：US17035370
申请日：2020-09-28
Applicant: FUJIFILM Corporation
Inventor： Yoshihisa USAMI , Yasuyuki ISHII , Yuki INOUE , Aya OUCHI
Abstract: The reaction apparatus includes a cell in which a specimen liquid of 1 μL or more is accommodated, a cooling plate structure that encloses the cell and consists of a cooling plate, a cooling unit that cools the cooling plate structure, and a heating unit that irradiates at least one of the cell or the specimen liquid with electromagnetic waves, in which the cooling plate configuring the cooling plate structure is in contact with at least two main surfaces of the cell, and the cooling plate structure includes a transparent window that transmits the electromagnetic waves.
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公开(公告)号：US20180355418A1
公开(公告)日：2018-12-13
申请号：US16110732
申请日：2018-08-23
Applicant: FUJIFILM Corporation
Inventor： Takayuki TSUJIMOTO , Aya OUCHI
IPC: C12Q1/6851
CPC classification number: C12Q1/6851 , C12N15/09 , C12Q1/68 , C12Q2600/16
Abstract: Provided is a chromosome number determination method including: a step of performing multiplex PCR for simultaneously amplifying a plurality of loci on chromosomes using genomic DNA extracted from a single cell or a small number of cells as templates, in which the multiplex PCR includes a plurality of thermal cycles including thermal denaturation, annealing, and elongation, annealing time is longer than or equal to 90 seconds and shorter than 1,500 seconds in at least one of the plurality of thermal cycles, a plurality of primer sets used in the multiplex PCR are designed through a method for designing primer sets used in a polymerase chain reaction including a first stage selection step based on a local alignment score and a second stage selection step based on a global alignment score.
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