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    CULTURE METHOD FOR CELLS AND PRODUCTION METHOD FOR USEFUL SUBSTANCE
    1.
    发明公开
    CULTURE METHOD FOR CELLS AND PRODUCTION METHOD FOR USEFUL SUBSTANCE 审中-公开

    公开(公告)号：US20240002784A1

    公开(公告)日：2024-01-04

    申请号：US18468223

    申请日：2023-09-15

    Applicant: FUJIFILM Corporation

    Inventor： Atsushi INADA ,  Kosuke TANIGUCHI ,  Naoto TAKAHASHI

    IPC: C12N5/00 ,  C12M1/34

    CPC classification number: C12N5/0018 ,  C12M41/42 ,  C12N2500/02

    Abstract: An object to be achieved by the present invention is to provide a culture method for cells, which makes it possible to suppress oxygen deficiency of cells in a case where the cell is cultured at a high density, and a production method for a useful substance using the culture method for cells. According to the present invention, there is provided a culture method for cells, in which a relationship between a stirring power P/V per unit volume, a dissolved oxygen concentration CL0, and a viable cell density VCD satisfies a condition of Expression 1, in cell culture in which the viable cell density is 60×106 cells/mL or more.


    0.0059×exp[0.0243×(P/V)]×CL0/VCD≥1.10×10−17   Expression 1:

    In the expression, L0 indicates a dissolved oxygen concentration [mol/mL]; VCD indicates a viable cell density [cells/mL]; and P/V indicates a stirring power [W/m3] per unit volume, which is defined by a predetermined expression.

    CELL CULTURE METHOD, PRODUCT PRODUCING METHOD, AND CELL CULTURE DEVICE
    3.
    发明申请
    CELL CULTURE METHOD, PRODUCT PRODUCING METHOD, AND CELL CULTURE DEVICE 有权

    公开(公告)号：US20210147781A1

    公开(公告)日：2021-05-20

    申请号：US17104094

    申请日：2020-11-25

    Applicant: FUJIFILM Corporation

    Inventor： Shinichi NAKAI ,  Kosuke TANIGUCHI ,  Nobuyuki HARAGUCHI ,  Tsukasa ISHIHARA

    IPC: C12M1/00 ,  C07K16/00

    Abstract: Provide are a cell culture method which includes using a culture vessel which contains a cell suspension containing cells and discharging a gas including 30% by volume or more of oxygen into the cell suspension from a sparger disposed in the culture vessel to culture the cells, in which an average hole diameter in a gas discharge portion in the sparger is 1 μm or more and 300 μm or less, and a surface area A (m2) in the gas discharge portion in the sparger, a volume X (m3) of the cell suspension in the culture vessel, and a flow rate Q (m3/min) of the gas that is discharged from the sparger satisfy a specific relationship, a product producing method including the cell culture method, and a cell culture device that enables the cell culture method.

    CELL CULTURE DEVICE, CELL CULTURE METHOD, AND PRODUCTION METHOD OF PRODUCT
    4.
    发明申请
    CELL CULTURE DEVICE, CELL CULTURE METHOD, AND PRODUCTION METHOD OF PRODUCT 有权

    公开(公告)号：US20220403308A1

    公开(公告)日：2022-12-22

    申请号：US17892655

    申请日：2022-08-22

    Applicant: FUJIFILM Corporation

    Inventor： Shinichi NAKAI ,  Nobuyuki HARAGUCHI ,  Kosuke TANIGUCHI

    IPC: C12M3/02 ,  C12M1/06 ,  C07K16/00 ,  C12N5/07 ,  B01F27/80

    Abstract: A cell culture device (1) includes: a culture vessel (10 ) that accommodates a cell suspension containing cells and a culture medium; and a stirring device (30) that is provided at a bottom part of the culture vessel (10) to stir the cell suspension accommodated in the culture vessel (10). The stirring device (30) has a shaft part (32), and a rotating part (33) rotatable with the shaft part (32) as a rotation axis. The rotating part (33) has a hole part (36) into which the shaft part (32) is inserted, and a gap (37) of 100 μm or more is secured between an interior wall demarcating the hole part (36) and the shaft part (32). A closing part (38) that closes an end part of the gap (37) in an axial direction of the rotation axis is provided.

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