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公开(公告)号：US20190247827A1
公开(公告)日：2019-08-15
申请号：US16402447
申请日：2019-05-03
Applicant: FUJIFILM Corporation
Inventor： Shiori KIHARA
CPC classification number: B01J20/286 , B01D15/305 , B01D15/36 , B01D15/362 , B01D15/38 , B01D15/3809 , B01J20/281 , C07K1/165 , C07K1/22 , C07K7/64 , C07K16/00
Abstract: A mixed mode affinity chromatography carrier includes a substrate, a hydrophilic polymer, an antibody-binding cyclic peptide, and a cation exchange group.
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公开(公告)号：US20240229015A1
公开(公告)日：2024-07-11
申请号：US18611981
申请日：2024-03-21
Applicant: FUJIFILM Corporation
Inventor： Yukiko ISHII , Jo TOMINAGA , Shiori KIHARA , Takahiro HOHSAKA , Takayoshi WATANABE
IPC: C12N15/10 , C12N15/113
CPC classification number: C12N15/1062 , C12N15/1044 , C12N15/1096 , C12N15/113
Abstract: Provided are a tRNA obtained by modifying a tRNA for tryptophan of Mycoplasma pneumoniae, the tRNA including a UUA as an anticodon to pair with a UAA codon, and an application of the tRNA.
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公开(公告)号：US20180229215A1
公开(公告)日：2018-08-16
申请号：US15952627
申请日：2018-04-13
Applicant: FUJIFILM Corporation
Inventor： Shiori KIHARA
IPC: B01J20/286 , C07K1/22 , C07K16/00 , B01D15/38
CPC classification number: B01J20/286 , B01D15/08 , B01D15/3809 , B01J20/24 , B01J20/28 , B01J20/285 , B01J20/291 , B01J20/3208 , B01J20/3217 , B01J20/3274 , B01J20/3293 , C07K1/22 , C07K16/00 , G01N30/88
Abstract: Provided is an affinity chromatography carrier having an excellent purification purity, including a substrate, a hydrophilic polymer, and an affinity ligand, in which the substrate is constituted of at least one selected from the group consisting of a polysaccharide, an acrylate-based polymer, a methacrylate-based polymer and a styrene-based polymer, the hydrophilic polymer is at least one selected from the group consisting of hydrophilic polysaccharides, the affinity ligand is at least one selected from the group consisting of an antibody-binding protein and an antibody-binding polypeptide, a carboxy group is introduced into the affinity chromatography carrier, and the amount of the carboxy group introduced is 15 mmol/L-gel to 60 mmol/L-gel in terms of ion exchange capacity.
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公开(公告)号：US20240247253A1
公开(公告)日：2024-07-25
申请号：US18597128
申请日：2024-03-06
Applicant: FUJIFILM Corporation
Inventor： Ken MATSUI , Jo TOMINAGA , Shiori KIHARA , Takahiro HOSAKA , Takayoshi WATANABE
IPC: C12N15/10
CPC classification number: C12N15/1037 , C12N15/1065
Abstract: Provided is a production method of a polypeptide in which expressing a polypeptide as a tagged polypeptide is included, the method including expressing the tagged polypeptide from a nucleic acid containing a base sequence encoding a tag consisting of an amino acid sequence set forth in SEQ ID NO: 1, which is arranged immediately after a start codon, and a base sequence encoding a polypeptide that is arranged in-frame downstream of the base sequence encoding the tag,
SEQ ID NO: 1: Val-Lys-Lys-(Xaa)n. (Xaa)n is a chain of n pieces of any amino acids, where n is an integer of 1 to 8, and the amino acids constituting (Xaa)n may be one type of amino acid or two or more types of amino acids.
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