公开(公告)号：US20190388896A1

公开(公告)日：2019-12-26

申请号：US16481409

申请日：2018-01-26

Applicant: FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION AT KOBE ,  JTEC Corporation

Inventor： Akihiko Taguchi ,  Yukiko Niino ,  Takashi Tsumura ,  Kenichi Morita

IPC: B01L3/00 ,  G01N1/34 ,  B01L3/02 ,  G01N1/14 ,  G01N33/50 ,  B04B5/04 ,  B04B11/04 ,  B04B13/00

Abstract: The mononuclear cell separation apparatus of the present invention has an injecting means (210) for injecting a centrifugation medium from the bottom surface of a container (100) storing a blood sample; a centrifugation means (300) for centrifuging a container (100) containing a centrifugation medium and a blood sample layered in this order from the bottom surface side; a detecting means (400) for detecting a clot present at a mononuclear cell layer after centrifugation; a removing means (220) for removing a detected clot; and a harvesting means (230) for harvesting the mononuclear cell. The mononuclear cell separation method of the present invention includes an injecting step, a centrifuging step, a detection step, a removing step, and a harvesting step corresponding to each constituent element of the mononuclear cell separation apparatus of the present invention.

公开(公告)号：US10441611B2

公开(公告)日：2019-10-15

申请号：US14912662

申请日：2014-08-18

Applicant: NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER ,  FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION AT KOBE ,  HYOGO COLLEGE OF MEDICINE ,  NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY ,  KANEKA CORPORATION

Inventor： Kenichi Yamahara ,  Akihiko Taguchi ,  Toshihiro Soma ,  Shunsuke Ohnishi ,  Akira Kobayashi

IPC: A61K35/50 ,  A61K35/28 ,  C12N5/0775 ,  A61K9/00 ,  A61L27/38 ,  C12N5/073 ,  C12N5/077 ,  A61L27/50

Abstract: An object of the present invention is to provide a method for producing a mesenchymal stromal cell composition, comprising conveniently and aseptically separating high-purity amnion-derived MSCs by performing enzyme treatment only once. According to the present invention, the following are provided: a method for producing a mesenchymal stromal cell composition, comprising: performing enzyme treatment of an amnion with collagenase and thermolysin and/or dispase; and filtering the enzyme-treated amnion through a mesh; a method for producing a cryopreserved mesenchymal stromal cell composition; and a therapeutic agent comprising as an active ingredient the mesenchymal stromal cell composition for a disease selected from graft-versus-host disease, inflammatory bowel disease, systemic lupus erythematosus, liver cirrhosis, or radiation enteritis.

公开(公告)号：US10398738B2

公开(公告)日：2019-09-03

申请号：US14912662

申请日：2014-08-18

Applicant: NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER ,  FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION AT KOBE ,  HYOGO COLLEGE OF MEDICINE ,  NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY ,  KANEKA CORPORATION

Inventor： Kenichi Yamahara ,  Akihiko Taguchi ,  Toshihiro Soma ,  Shunsuke Ohnishi ,  Akira Kobayashi

IPC: A61K35/50 ,  A61K35/28 ,  C12N5/0775 ,  C12N5/073 ,  A61K9/00 ,  A61L27/38 ,  A61L27/50 ,  C12N5/077

Abstract: An object of the present invention is to provide a method for producing a mesenchymal stromal cell composition, comprising conveniently and aseptically separating high-purity amnion-derived MSCs by performing enzyme treatment only once. According to the present invention, the following are provided: a method for producing a mesenchymal stromal cell composition, comprising: performing enzyme treatment of an amnion with collagenase and thermolysin and/or dispase; and filtering the enzyme-treated amnion through a mesh; a method for producing a cryopreserved mesenchymal stromal cell composition; and a therapeutic agent comprising as an active ingredient the mesenchymal stromal cell composition for a disease selected from graft-versus-host disease, inflammatory bowel disease, systemic lupus erythematosus, liver cirrhosis, or radiation enteritis.

公开(公告)号：US11744858B2

公开(公告)日：2023-09-05

申请号：US15558999

申请日：2016-03-18

Applicant: Foundation for Biomedical Research and Innovation at Kobe

Inventor： Akihiko Taguchi ,  Kenichi Yamahara ,  Yukiko Niino

IPC: A61K35/14 ,  A61P9/10 ,  A61K35/28 ,  A61K45/00 ,  A61K38/48 ,  A61K38/22 ,  A61K38/46 ,  A61P13/12 ,  A61K38/17 ,  A61K38/18 ,  C12N5/00

CPC classification number: A61K35/14 ,  A61K35/28 ,  A61K38/1709 ,  A61K38/1825 ,  A61K38/1841 ,  A61K38/1858 ,  A61K38/1866 ,  A61K38/22 ,  A61K38/46 ,  A61K38/48 ,  A61K38/4886 ,  A61K45/00 ,  A61P9/10 ,  A61P13/12 ,  C12N5/0087 ,  C12Y304/24024

Abstract: The present invention provides a sufficiently effective medicine for treatment and/or prevention of ischemic diseases, without performing isolation of therapeutic cells or removal of deleterious cells from blood cells/hemocytes. The blood cells and/or the hemocytes are subjected to the action of a saccharide. The saccharide is a monosaccharide, a disaccharide, a trisaccharide, a polysaccharides, or a copolymer containing a monosaccharide, a disaccharide, or a trisaccharide as a component. The saccharide is a copolymer of sucrose and epichlorohydrin.