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公开(公告)号:US06506594B1
公开(公告)日:2003-01-14
申请号:US09526992
申请日:2000-03-16
申请人: Francis Barany , Norman P. Gerry , Nancy E. Witowski , Joseph Day , Robert P. Hammer , George Barany
发明人: Francis Barany , Norman P. Gerry , Nancy E. Witowski , Joseph Day , Robert P. Hammer , George Barany
IPC分类号: C12M134
CPC分类号: C12Q1/6837 , B01J2219/00313 , B01J2219/00326 , B01J2219/00416 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00599 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00722 , B01J2219/00729 , C12Q1/6827 , C12Q1/6858 , C40B40/06 , C40B50/08 , C40B60/14 , C12Q2565/514 , C12Q2565/125 , C12Q2561/125
摘要: The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.