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公开(公告)号:US20050003471A1
公开(公告)日:2005-01-06
申请号:US10613767
申请日:2003-07-03
申请人: Fu-Sheng Wang , Mark Dorfner , Suma Peesapati , Steve Burger , Kojiro Hirai , Yukio Hamaguchi
发明人: Fu-Sheng Wang , Mark Dorfner , Suma Peesapati , Steve Burger , Kojiro Hirai , Yukio Hamaguchi
IPC分类号: C12Q1/02 , C12R1/91 , G01N1/30 , G01N15/10 , G01N15/12 , G01N15/14 , G01N21/64 , G01N33/49 , G01N33/50 , G01N33/48
CPC分类号: G01N21/47 , G01N1/30 , G01N15/12 , G01N15/147 , G01N21/6428 , G01N33/5094 , G01N2015/1037 , G01N2015/105 , G01N2015/1087
摘要: Methods of detecting megakaryocytes are described that include (a) providing a sample that contains a cell; (b) detecting a plurality of morphological information from the cell; (c) generating a scattergram from the plurality of morphological information; and (d) determining whether a population exists in a megakaryocyte region of the scattergram.
摘要翻译: 描述了检测巨核细胞的方法,其包括(a)提供含有细胞的样品; (b)从小区检测多个形态信息; (c)从所述多个形态信息生成散点图; 和(d)确定群体是否存在于散点图的巨核细胞区域中。
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公开(公告)号:USRE39006E1
公开(公告)日:2006-03-07
申请号:US10704463
申请日:2003-11-07
申请人: Berend Houwen , Yukio Tsujino , Takashi Morikawa , Yoshiro Ikeuchi , Yukio Hamaguchi , Fu-sheng Wang , Kojiro Hirai
发明人: Berend Houwen , Yukio Tsujino , Takashi Morikawa , Yoshiro Ikeuchi , Yukio Hamaguchi , Fu-sheng Wang , Kojiro Hirai
IPC分类号: C12Q1/04
CPC分类号: G01N33/5094
摘要: Improved method for detecting and/or counting the appearance of hematopoietic progenitor cells which comprises the steps of mixing a blood sample with a reagent capable of detecting immature cells without employing any immunological techniques, obtaining cell information about the thus treated sample using a particle analyzer and constructing a cell distribution profile, delineating a portion of the profile as a zone in which at least one subclass of hematopoietic progenitor cells appear, and counting the cells within the zone. The method is capable of detecting the appearance of hematopoietic progenitor cells in a simple and positive manner.
摘要翻译: 用于检测和/或计数造血祖细胞外观的改进方法,其包括以下步骤:将血液样品与能够检测未成熟细胞的试剂混合而不使用任何免疫技术,使用粒子分析仪获得关于如此处理的样品的细胞信息; 构建细胞分布轮廓,将轮廓的一部分描绘为其中存在至少一个造血祖细胞亚类的区域,并对该区域内的细胞进行计数。 该方法能够以简单和积极的方式检测造血祖细胞的外观。
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