公开(公告)号：US07998752B2

公开(公告)日：2011-08-16

申请号：US11950706

申请日：2007-12-05

申请人： Mie Takahashi ,  Masataka Nadaoka ,  Hirotaka Tanaka ,  Fumihisa Kitawaki

发明人： Mie Takahashi ,  Masataka Nadaoka ,  Hirotaka Tanaka ,  Fumihisa Kitawaki

IPC分类号： G01N33/53

CPC分类号： G01N30/90 ,  G01N33/5002 ,  G01N33/5005 ,  G01N33/558 ,  Y10S436/808 ,  Y10S436/81

摘要： According to the biosensor and the blood component analytical method of the present invention, in a biosensor that is made of a single layer or plural layers of a porous material as shown in FIG. 1, having a reagent holding part and utilizing chromatography, a cell shrinkage reagent is carried on at least part of the reagent holding part, or at least part of a chromatographically developed part that is upstream of the reagent holding part. According to the biosensor having the above-mentioned structure and the blood component analytical method, even when whole blood is a sample, a high-accuracy blood component analysis cart be performed easily and quickly with less cost.

摘要翻译： 根据本发明的生物传感器和血液成分分析方法，在图1所示的由单层或多层多孔质材料构成的生物传感器中， 如图1所示，具有试剂保持部并利用色谱法，在试剂保持部的至少一部分或者在试剂保持部的上游的色谱显影部的至少一部分上承载有细胞收缩试剂。 根据具有上述结构的生物传感器和血液成分分析方法，即使全血是样品，也能够以更低的成本容易且快速地进行高精度的血液成分分析手推车。

公开(公告)号：US07790439B2

公开(公告)日：2010-09-07

申请号：US10416272

申请日：2002-08-09

申请人： Masataka Nadaoka ,  Mie Takahashi ,  Hirotaka Tanaka ,  Fumihisa Kitawaki

发明人： Masataka Nadaoka ,  Mie Takahashi ,  Hirotaka Tanaka ,  Fumihisa Kitawaki

IPC分类号： G01N33/558

CPC分类号： G01N33/558

摘要： In a biosensor having a developing layer for developing a sample solution, including a reagent part immobilized to a portion of the developing layer and a marked reagent part which is held in a dry state by a portion of the developing layer, and is dissolvable by developing the sample solution, and qualitatively or quantitatively analyzing an analyte in the sample solution by measuring the amount of the marker reagent bound to the reagent immobilization part; wherein plural reagent immobilization parts exist, and the respective reagent immobilization parts have different affinities for the analyte in the sample solution or the marker reagent, whereby a prozone phenomenon can be detected. Further, a biosensor with a high precision of measurement, which has a wider dynamic range for the concentration of the analyte in the sample solution, can be provided. Thereby, a highly accurate and precise biosensor which can detect a prozone phenomenon even when the range of the concentration of the analyte in the sample solution is wide, and has a wide measurement dynamic range can be provided.

摘要翻译： 在具有用于显影样品溶液的显影层的生物传感器中，包括固定在显影层的一部分上的试剂部分和由显影层的一部分保持在干燥状态的标记的试剂部分，并且可通过显影 样品溶液，并通过测量与试剂固定部分结合的标记试剂的量定性或定量分析样品溶液中的分析物; 其中存在多个试剂固定部分，并且各试剂固定部分对于样品溶液或标记试剂中的分析物的亲和力不同，从而可以检测到前区现象。 此外，可以提供具有高测量精度的生物传感器，其具有用于样品溶液中的分析物浓度的更宽的动态范围。 因此，即使在样品溶液中的分析物的浓度范围宽的情况下也能够检测前区现象的高精度且精确的生物传感器，并且具有宽的测量动态范围。

公开(公告)号：US20080292502A1

公开(公告)日：2008-11-27

申请号：US11910513

申请日：2006-03-24

申请人： Fumihisa Kitawaki ,  Hirotaka Tanaka ,  Kenji Watanabe

发明人： Fumihisa Kitawaki ,  Hirotaka Tanaka ,  Kenji Watanabe

IPC分类号： B04B5/10 ,  B01F15/02

CPC分类号： B01F3/08 ,  B01F13/0059 ,  B01F15/0233 ,  B01F2215/0037 ,  G01N21/07 ,  G01N35/00069 ,  G01N2035/00495 ,  G01N2035/00524 ,  Y10T436/111666 ,  Y10T436/2575

摘要： Herein disclosed is a liquid homogenizer which can evenly mix liquids with small space.There is provided a liquid homogenizer for mixing two or more liquids, comprising: a rotator (1) rotatable around a rotation axis; at least two liquid-mixing chambers (6, 10) formed in the rotator, and being different from each other in distance from the rotation axis; and at least two channels (8) through which one of the liquid-mixing chambers is communicated with the other of the liquid-mixing chambers (6, 10), wherein the liquid-mixing chambers (6, 10) include an outer liquid-mixing chamber (10) and an inner liquid-mixing chamber (6) close to the rotation axis in comparison with the outer liquid-mixing chamber, when the rotator (1) is rotating around the rotation axis, the liquids are shifted by a centrifugal force to the outer liquid-mixing chamber from the inner liquid-mixing chamber through the channels (8), and agitated to be mixed by turbulent flows in the outer liquid-mixing chamber.

摘要翻译： 本文公开了一种液体均化器，其可以均匀地混合具有小空间的液体。 提供了一种用于混合两种或更多种液体的液体均化器，包括：可围绕旋转轴线旋转的旋转器（1）; 形成在旋转体中的至少两个液体混合室（6,10），并且与旋转轴线的距离彼此不同; 和至少两个通道（8），其中一个液体混合室通过该通道与另一个液体混合室（6,10）连通，其中液体混合室（6,10）包括外部液体 - 混合室（10）和与外部液体混合室相比靠近旋转轴线的内部混合室（6），当旋转体（1）围绕旋转轴线旋转时，液体被离心 通过通道（8）从内部液体混合室向外部液体混合室施力，并搅拌以在外部混合室中被湍流混合。

公开(公告)号：US20100267048A1

公开(公告)日：2010-10-21

申请号：US12740857

申请日：2008-10-29

申请人： Hirotaka Tanaka ,  Masanori Tanaka ,  Chizu Asahara ,  Fumihisa Kitawaki

发明人： Hirotaka Tanaka ,  Masanori Tanaka ,  Chizu Asahara ,  Fumihisa Kitawaki

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： G01N33/721 ,  G01N33/541 ,  G01N33/543 ,  G01N33/723 ,  G01N33/726 ,  Y10T436/105831 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375

摘要： Hemoglobin in a sample solution is quickly and reliably denatured; at the same time, quick and accurate measurement of hemoglobin and a hemoglobin derivative is realized. In a method for measuring hemoglobin and a hemoglobin derivative, and a reagent composition, a measurement kit, an analysis device, and an analysis system used in the method, a sample solution containing a blood component is treated with a nonionic surfactant, an oxidizing agent, and a metal salt to denature hemoglobin in the sample solution to measure the hemoglobin, and thereafter the amount of a hemoglobin derivative in the sample is measured by an immunological method using an antibody specifically binding to a denatured site of the denatured hemoglobin derivative.

摘要翻译： 样品溶液中的血红蛋白迅速可靠地变性; 同时，实现血红蛋白和血红蛋白衍生物的快速准确测定。 在本方法中使用的血红蛋白和血红蛋白衍生物，试剂组合物，测定试剂盒，分析装置和分析系统的测定方法中，将含有血液成分的试样溶液用非离子性表面活性剂，氧化剂 和金属盐，使样品溶液中的血红蛋白变性，测定血红蛋白，然后用特异性结合变性血红蛋白衍生物的变性部位的抗体的免疫学方法测定样品中的血红蛋白衍生物的量。

公开(公告)号：US07754151B2

公开(公告)日：2010-07-13

申请号：US11910513

申请日：2006-03-24

申请人： Fumihisa Kitawaki ,  Hirotaka Tanaka ,  Kenji Watanabe

发明人： Fumihisa Kitawaki ,  Hirotaka Tanaka ,  Kenji Watanabe

IPC分类号： G01N9/30

CPC分类号： B01F3/08 ,  B01F13/0059 ,  B01F15/0233 ,  B01F2215/0037 ,  G01N21/07 ,  G01N35/00069 ,  G01N2035/00495 ,  G01N2035/00524 ,  Y10T436/111666 ,  Y10T436/2575

摘要： Herein disclosed is a liquid homogenizer for mixing two or more liquids, comprising: a rotator (1) rotatable around a rotation axis; at least two liquid-mixing chambers (6, 10) formed in the rotator, and being different from each other in distance from the rotation axis; and at least two channels (8) through which one of the liquid-mixing chambers is communicated with the other of the liquid-mixing chambers (6, 10), wherein the liquid-mixing chambers (6, 10) include an outer liquid-mixing chamber (10) and an inner liquid-mixing chamber (6) close to the rotation axis in comparison with the outer liquid-mixing chamber, when the rotator (1) is rotating around the rotation axis, the liquids are shifted by a centrifugal force to the outer liquid-mixing chamber from the inner liquid-mixing chamber through the channels (8), and agitated to be mixed by turbulent flows in the outer liquid-mixing chamber.

摘要翻译： 本文公开了一种用于混合两种或更多种液体的液体均化器，包括：可围绕旋转轴线旋转的旋转体（1） 形成在旋转体中的至少两个液体混合室（6,10），并且与旋转轴线的距离彼此不同; 和至少两个通道（8），其中一个液体混合室通过该通道与另一个液体混合室（6,10）连通，其中液体混合室（6,10）包括外部液体 - 混合室（10）和与外部液体混合室相比靠近旋转轴线的内部混合室（6），当旋转体（1）围绕旋转轴线旋转时，液体被离心 通过通道（8）从内部液体混合室向外部液体混合室施力，并搅拌以在外部混合室中被湍流混合。

公开(公告)号：US07575915B2

公开(公告)日：2009-08-18

申请号：US10031988

申请日：2001-05-25

申请人： Masataka Nadaoka ,  Mie Takahashi ,  Hirotaka Tanaka ,  Fumihisa Kitawaki

发明人： Masataka Nadaoka ,  Mie Takahashi ,  Hirotaka Tanaka ,  Fumihisa Kitawaki

IPC分类号： G01N33/558

CPC分类号： G01N33/558 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/805 ,  Y10S436/807

摘要： A biosensor is provided with a development layer for developing an inspection target solution, that has a reagent immobilization part where an antibody for a measurement target in the inspection target solution is immobilized and a reagent holding part which marks in a dry state and holds an antibody which can be eluted by development of the inspection target solution in parts of the development layer. The biosensor measures a bonding amount of the marker reagent bonded to the reagent immobilization part, thereby qualitatively or quantitatively measuring components to be measured in the inspection target solution, and is further provided with a space forming material that forms a cavity part, which is a space into which the inspection target solution flows, between the development layer for developing the inspection target solution and the space forming material.

摘要翻译： 生物传感器设置有用于开发检查对象溶液的显影层，其具有固定有检查对象溶液中的测定对象的抗体的试剂固定部以及标记为干燥状态并保持抗体的试剂保持部 其可以通过在显影层的部分中开发检查目标溶液而被洗脱。 生物传感器测定与试剂固定部接合的标记试剂的结合量，从而定量或定量地测量检查对象溶液中待测量的成分，并且还设置有空间形成材料，其形成空腔部分，其为 检查对象溶液流动的空间，用于显影检查对象溶液的显影层和空间形成材料之间。

公开(公告)号：US08921052B2

公开(公告)日：2014-12-30

申请号：US11918394

申请日：2006-04-12

申请人： Hirotaka Tanaka ,  Masanori Tanaka ,  Fumihisa Kitawaki

发明人： Hirotaka Tanaka ,  Masanori Tanaka ,  Fumihisa Kitawaki

IPC分类号： G01N33/53 ,  C12M1/00 ,  G01N33/72

CPC分类号： G01N33/72 ,  G01N33/5302 ,  G01N33/721

摘要： A sample solution including blood components is processed with a denaturalization reagent comprising a nonionic surface-activating agent and an oxidizing agent to denaturalize a hemoglobin derivative in the sample solution, and thereafter, an immunoassay is performing utilizing an antibody that is specific to a denaturalized site of the hemoglobin derivative to measure the amount of the hemoglobin derivative in the sample. Therefore, when performing assay of hemoglobin derivative, denaturalization of hemoglobin can be performed speedily and reliably while minimizing adverse effects of the denaturalization reagent on immune reaction.

摘要翻译： 包含血液成分的样品溶液用包含非离子表面活性剂和氧化剂的变性试剂处理以使样品溶液中的血红蛋白衍生物变性，然后利用对变性部位特异性的抗体进行免疫测定 的血红蛋白衍生物以测量样品中血红蛋白衍生物的量。 因此，当进行血红蛋白衍生物的测定时，可以快速可靠地进行血红蛋白的变性，同时使变性反应物对免疫反应的不良影响最小化。

公开(公告)号：US20080293074A1

公开(公告)日：2008-11-27

申请号：US11918394

申请日：2006-04-12

申请人： Hirotaka Tanaka ,  Masanori Tanaka ,  Fumihisa Kitawaki

发明人： Hirotaka Tanaka ,  Masanori Tanaka ,  Fumihisa Kitawaki

IPC分类号： G01N33/53 ,  C12M1/00

CPC分类号： G01N33/72 ,  G01N33/5302 ,  G01N33/721

摘要： A sample solution including blood components is processed with a denaturalization reagent comprising a nonionic surface-activating agent and an oxidizing agent to denaturalize a hemoglobin derivative in the sample solution, and thereafter, an immunoassay is performing utilizing an antibody that is specific to a denaturalized site of the hemoglobin derivative to measure the amount of the hemoglobin derivative in the sample. Therefore, when performing assay of hemoglobin derivative, denaturalization of hemoglobin can be performed speedily and reliably while minimizing adverse effects of the denaturalization reagent on immune reaction.

摘要翻译： 包含血液成分的样品溶液用包含非离子表面活性剂和氧化剂的变性试剂处理以使样品溶液中的血红蛋白衍生物变性，然后利用对变性部位特异性的抗体进行免疫测定 的血红蛋白衍生物以测量样品中血红蛋白衍生物的量。 因此，当进行血红蛋白衍生物的测定时，可以快速可靠地进行血红蛋白的变性，同时使变性反应物对免疫反应的不良影响最小化。

公开(公告)号：US20080085561A1

公开(公告)日：2008-04-10

申请号：US11950706

申请日：2007-12-05

申请人： Mie Takahashi ,  Masataka Nadaoka ,  Hirotaka Tanaka ,  Fumihisa Kitawaki

发明人： Mie Takahashi ,  Masataka Nadaoka ,  Hirotaka Tanaka ,  Fumihisa Kitawaki

IPC分类号： G01N30/02 ,  G01N33/50

CPC分类号： G01N30/90 ,  G01N33/5002 ,  G01N33/5005 ,  G01N33/558 ,  Y10S436/808 ,  Y10S436/81

摘要： According to the biosensor and the blood component analytical method of the present invention, in a biosensor that is made of a single layer or plural layers of a porous material as shown in FIG. 1, having a reagent holding part and utilizing chromatography, a cell shrinkage reagent is carried on at least part of the reagent holding part, or at least part of a chromatographically developed part that is upstream of the reagent holding part. According to the biosensor having the above-mentioned structure and the blood component analytical method, even when whole blood is a sample, a high-accuracy blood component analysis can be performed easily and quickly with less cost.

摘要翻译： 根据本发明的生物传感器和血液成分分析方法，在图1所示的由单层或多层多孔质材料构成的生物传感器中， 如图1所示，具有试剂保持部并利用色谱法，在试剂保持部的至少一部分或者在试剂保持部的上游的色谱显影部的至少一部分上承载有细胞收缩试剂。 根据具有上述结构和血液成分分析方法的生物传感器，即使全血是样品，也能够以较低的成本容易且快速地进行高精度的血液成分分析。

公开(公告)号：US20070292964A1

公开(公告)日：2007-12-20

申请号：US11574315

申请日：2005-09-08

申请人： Fumihisa Kitawaki ,  Hirotaka Tanaka

发明人： Fumihisa Kitawaki ,  Hirotaka Tanaka

IPC分类号： G01N21/17

CPC分类号： B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0806 ,  B01L2400/0409 ,  G01N21/59 ,  G01N33/54373 ,  G01N35/00069

摘要： Herein disclosed is a measuring equipment, comprising: a light source (13); a light receiving unit (14) disposed in spaced relationship with the light source (13); a device (11) disposed between the light source (13) and the light receiving unit (14); the device (11) having a reaction part (12) for accommodating therein a solid phase support (121), a test substance in a specimen, and a test reagent operative to react with any one of the solid phase support (121) and the test substance, the solid phase support (121) having a surface having a specific binding substance fixed thereon, the specific binding substance being specifically reactive with the test substance, in which, the specific binding substance is adapted to have a plurality of concavities and convexities (123) formed on the solid phase support with the test reagent and the test substance introduced into the reaction part (12) and reacted with each other, the light source (13) is operative to produce a light to transmit through the solid phase support (121) and scan the device (11), the light transmitted through the solid phase support (121) having a signal indicative of the concavities and convexities (123) on the solid phase support (121), the signal of the light indicative of the concavities and convexities (123) transmitted through the solid phase support (121) being detected by the light receiving unit (14).

摘要翻译： 本文公开了一种测量设备，包括：光源（13）; 与光源（13）间隔开设置的光接收单元（14）; 设置在光源（13）和光接收单元（14）之间的装置（11）; 所述装置（11）具有用于容纳固相载体（121）的反应部分（12），试样中的试验物质和可与任一种固相载体（121）和 所述固体相载体（121）具有固定有特异性结合物质的表面，所述特异性结合物质与被检物质特异性反应，特异性结合物质适于具有多个凹凸 （123），其与所述测试试剂和所述测试物质在所述固相载体上形成并引入到所述反应部分（12）中并且彼此反应，所述光源（13）可操作以产生透射通过所述固相载体 （121）并扫描所述装置（11），透射通过所述固相支架（121）的光具有指示所述固相支撑件（121）上的凹凸（123）的信号，所述s 点亮由光接收单元（14）检测到的表示透过固相支撑件（121）的凹凸的光。