公开(公告)号：US20150080554A1

公开(公告)日：2015-03-19

申请号：US14385336

申请日：2013-03-26

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Mats Ander ,  Goran Bauren ,  Tomas Bjorkman ,  Per-Mikael Aberg ,  Gustav Rodrigo

IPC: C07K16/00 ,  C07K14/31 ,  G01N33/68 ,  B01J20/291

CPC classification number: C07K16/00 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/291 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J2220/80 ,  C07K1/22 ,  C07K14/31 ,  G01N33/6854

Abstract: The invention discloses an immunoglobulin-binding protein comprising one or more mutated immunoglobulin-binding domains (monomers) of staphylococcal Protein A (E, D, A, B, C) or protein Z or a functional variant thereof, wherein in at least one of the one or more mutated monomers, the asparagine or histidine at the position corresponding to H18 of the B domain of Protein A or of Protein Z has been deleted or substituted with a first amino acid residue which is not proline or asparagine and wherein, if the amino acid residue at position 57 is proline and the amino acid residue at position 28 is asparagine, then the amino acid residue at the position corresponding to H18 of the B domain of protein A or of protein Z is not serine, threonine or lysine.

Abstract translation: 本发明公开了一种免疫球蛋白结合蛋白，其包含葡萄球菌蛋白A（E，D，A，B，C）或蛋白Z或其功能变体的一个或多个突变的免疫球蛋白结合结构域（单体），其中至少一个 一个或多个突变单体，在对应于蛋白A或蛋白Z的B结构域的H18的位置处的天冬酰胺或组氨酸已被缺失或被不是脯氨酸或天冬酰胺的第一氨基酸残基取代，并且其中，如果 第57位的氨基酸残基是脯氨酸，28位的氨基酸残基是天冬酰胺，则相应于蛋白A的B结构域或蛋白Z的H18位置的氨基酸残基不是丝氨酸，苏氨酸或赖氨酸。

公开(公告)号：US20160200797A1

公开(公告)日：2016-07-14

申请号：US15063471

申请日：2016-03-07

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Martin Hall ,  Sture Larsson ,  Andreas Muranyi ,  Gustav Rodrigo ,  Jinyu Zou ,  Per-Mikael Aberg

IPC: C07K16/00 ,  B01D15/38 ,  C07K1/22

CPC classification number: B01J20/24 ,  B01D15/3809 ,  B01J20/28019 ,  B01J20/285 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/52 ,  B01J2220/54 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  C07K17/10 ,  C07K2317/55

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

Abstract translation: 本发明涉及一种色谱配体，其包含来自葡萄球菌蛋白A（SpA）的结构域C，或其功能片段或变体。 色谱配体具有在现场（CIP）条件下耐受苛刻清洁的有利能力，并且能够结合抗体的Fab片段。 配体可以具有末端偶联基团，例如精氨酸或半胱氨酸，以促进其与不溶性载体例如珠粒或膜的偶联。 本发明还涉及在分离抗体中使用配体的方法以及可以包括洗涤步骤和/或用碱再生的纯化方案。

公开(公告)号：US09290549B2

公开(公告)日：2016-03-22

申请号：US14164519

申请日：2014-01-27

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Martin Hall ,  Sture Larsson ,  Andreas Muranyi ,  Gustav Rodrigo ,  Jinyu Zou ,  Per-Mikael Aberg

IPC: C07K17/00 ,  C07K14/31 ,  B01D15/38 ,  B01J20/286 ,  C07K1/22 ,  B01J20/32 ,  C07K16/00

CPC classification number: B01J20/24 ,  B01D15/3809 ,  B01J20/28019 ,  B01J20/285 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/52 ,  B01J2220/54 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  C07K17/10 ,  C07K2317/55

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

Abstract translation: 本发明涉及一种色谱配体，其包含来自葡萄球菌蛋白A（SpA）的结构域C，或其功能片段或变体。 色谱配体具有在现场（CIP）条件下耐受苛刻清洁的有利能力，并且能够结合抗体的Fab片段。 配体可以具有末端偶联基团，例如精氨酸或半胱氨酸，以促进其与不溶性载体例如珠粒或膜的偶联。 本发明还涉及在分离抗体中使用配体的方法以及可以包括洗涤步骤和/或用碱再生的纯化方案。