公开(公告)号：US09663558B2

公开(公告)日：2017-05-30

申请号：US14904077

申请日：2014-07-10

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Mats Ander ,  Goran Bauren ,  Tomas Bjorkman ,  Gustav Rodrigo

IPC: C07K14/31 ,  C07K1/22 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32 ,  C07K14/745 ,  C07K16/00

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/745 ,  C07K16/00

Abstract: A polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A, as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 15 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of the polypeptide, as well as separation matrices comprising the multimers or polypeptides.

公开(公告)号：US20150080554A1

公开(公告)日：2015-03-19

申请号：US14385336

申请日：2013-03-26

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Mats Ander ,  Goran Bauren ,  Tomas Bjorkman ,  Per-Mikael Aberg ,  Gustav Rodrigo

IPC: C07K16/00 ,  C07K14/31 ,  G01N33/68 ,  B01J20/291

CPC classification number: C07K16/00 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/291 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J2220/80 ,  C07K1/22 ,  C07K14/31 ,  G01N33/6854

Abstract: The invention discloses an immunoglobulin-binding protein comprising one or more mutated immunoglobulin-binding domains (monomers) of staphylococcal Protein A (E, D, A, B, C) or protein Z or a functional variant thereof, wherein in at least one of the one or more mutated monomers, the asparagine or histidine at the position corresponding to H18 of the B domain of Protein A or of Protein Z has been deleted or substituted with a first amino acid residue which is not proline or asparagine and wherein, if the amino acid residue at position 57 is proline and the amino acid residue at position 28 is asparagine, then the amino acid residue at the position corresponding to H18 of the B domain of protein A or of protein Z is not serine, threonine or lysine.

Abstract translation: 本发明公开了一种免疫球蛋白结合蛋白，其包含葡萄球菌蛋白A（E，D，A，B，C）或蛋白Z或其功能变体的一个或多个突变的免疫球蛋白结合结构域（单体），其中至少一个 一个或多个突变单体，在对应于蛋白A或蛋白Z的B结构域的H18的位置处的天冬酰胺或组氨酸已被缺失或被不是脯氨酸或天冬酰胺的第一氨基酸残基取代，并且其中，如果 第57位的氨基酸残基是脯氨酸，28位的氨基酸残基是天冬酰胺，则相应于蛋白A的B结构域或蛋白Z的H18位置的氨基酸残基不是丝氨酸，苏氨酸或赖氨酸。