公开(公告)号：US20160207966A1

公开(公告)日：2016-07-21

申请号：US14904077

申请日：2014-07-10

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Mats ANDER ,  Goran BAUREN ,  Tomas BJORKMAN ,  Gustav RODRIGO

IPC: C07K14/31

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/745 ,  C07K16/00

Abstract: A polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A, as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 15 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of the polypeptide, as well as separation matrices comprising the multimers or polypeptides.

Abstract translation: 具有改善的碱稳定性的多肽，所述多肽包含SEQ ID NO 1或SEQ ID NO 2所规定的葡萄球菌蛋白A的B或C结构域或如SEQ ID NO 3所定义的蛋白Z的突变体，其中 至少15位的谷氨酰胺残基已被突变成除天冬酰胺之外的氨基酸。 本发明还公开了多肽的多聚体，以及包含多聚体或多肽的分离基质。

公开(公告)号：US20170320922A1

公开(公告)日：2017-11-09

申请号：US15598685

申请日：2017-05-18

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Mats ANDER ,  Goran BAUREN ,  Tomas BJORKMAN ,  Gustav RODRIGO

IPC: C07K14/31 ,  C07K14/745 ,  C07K1/22 ,  B01J20/32 ,  B01D15/38 ,  B01J20/286 ,  C07K16/00

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/745 ,  C07K16/00

Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamic acid residue at position 15 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.

公开(公告)号：US20160159855A1

公开(公告)日：2016-06-09

申请号：US14903837

申请日：2014-07-08

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Tomas BJORKMAN ,  Goran Bauren

IPC: C07K1/22 ,  C07K14/745 ,  C07K16/00 ,  C07K14/31

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/745 ,  C07K16/00

Abstract: A polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A, as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.

Abstract translation: 具有改善的碱稳定性的多肽，所述多肽包含SEQ ID NO 1或SEQ ID NO 2所规定的葡萄球菌蛋白A的B或C结构域或如SEQ ID NO 3所定义的蛋白Z的突变体，其中 至少第9位的谷氨酰胺残基已被突变为除天冬酰胺之外的氨基酸。 本发明还公开了所述多肽的多聚体，以及包含多聚体或多肽的分离基质。