公开(公告)号：US20190339261A1

公开(公告)日：2019-11-07

申请号：US16470677

申请日：2017-12-21

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Ola Lind ,  Klaus Gebauer ,  Eric Faldt ,  Nils Norrman ,  Ronnie Palmgren ,  Jesper Hedberg ,  Niklas Jungnelius ,  Karl Liderfelt ,  Anita Solbrand

IPC: G01N33/543 ,  C12M1/00 ,  C12M3/00 ,  C12M3/06 ,  C12M1/34

Abstract: The invention discloses an immunoglobulin-binding magnetic bead, comprising a porous matrix and one or more magnetic particles embedded in said matrix, wherein said matrix comprises a porous polymer and at least 10 mg/ml Fc-binding proteinaceous ligands covalently coupled to said porous polymer.

公开(公告)号：US11846635B2

公开(公告)日：2023-12-19

申请号：US16470677

申请日：2017-12-21

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Ola Lind ,  Klaus Gebauer ,  Eric Faldt ,  Nils Norrman ,  Ronnie Palmgren ,  Jesper Hedberg ,  Niklas Jungnelius ,  Karl Liderfelt ,  Anita Solbrand

IPC: G01N33/543 ,  C12M3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/34 ,  C12N13/00 ,  B03C5/00 ,  B03C5/02 ,  B01D21/00 ,  B03C1/30 ,  B03C1/01 ,  B03C1/28

CPC classification number: G01N33/54326 ,  B01D21/0009 ,  B03C1/30 ,  B03C5/00 ,  B03C5/02 ,  C12M23/48 ,  C12M27/16 ,  C12M29/00 ,  C12M41/44 ,  C12M45/07 ,  C12M47/04 ,  C12M47/10 ,  C12N13/00 ,  G01N33/5436 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  G01N2333/31

Abstract: The invention discloses an immunoglobulin-binding magnetic bead, comprising a porous matrix and one or more magnetic particles embedded in said matrix, wherein said matrix comprises a porous polymer and at least 10 mg/ml Fc-binding proteinaceous ligands covalently coupled to said porous polymer.

公开(公告)号：US20200087614A1

公开(公告)日：2020-03-19

申请号：US16470607

申请日：2017-12-27

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Ola Lind ,  Johan Fredrik Avallin ,  Andreas Torbjorn Lundin ,  Nils Norrman ,  Ronnie Palmgren ,  Gustav Jose Rodrigo

IPC: C12M1/00 ,  C12N13/00

Abstract: A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: —providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; —separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; —forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); —eluting the bound biomolecules in the elution cell.

公开(公告)号：US11686726B2

公开(公告)日：2023-06-27

申请号：US16470607

申请日：2017-12-27

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Ola Lind ,  Johan Fredrik Avallin ,  Andreas Torbjorn Lundin ,  Nils Norrman ,  Ronnie Palmgren ,  Gustav Jose Rodrigo

IPC: G01N33/543 ,  C12M3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/34 ,  C12N13/00 ,  B01D21/00 ,  B03C5/00 ,  B03C5/02 ,  B03C1/30 ,  B03C1/28 ,  B03C1/01

CPC classification number: G01N33/54326 ,  B01D21/0009 ,  B03C1/30 ,  B03C5/00 ,  B03C5/02 ,  C12M23/48 ,  C12M27/16 ,  C12M29/00 ,  C12M41/44 ,  C12M45/07 ,  C12M47/04 ,  C12M47/10 ,  C12N13/00 ,  G01N33/5436 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  G01N2333/31

Abstract: A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: —providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; —separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; —forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); —eluting the bound biomolecules in the elution cell.