公开(公告)号：US20200087614A1

公开(公告)日：2020-03-19

申请号：US16470607

申请日：2017-12-27

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Ola Lind ,  Johan Fredrik Avallin ,  Andreas Torbjorn Lundin ,  Nils Norrman ,  Ronnie Palmgren ,  Gustav Jose Rodrigo

IPC: C12M1/00 ,  C12N13/00

Abstract: A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: —providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; —separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; —forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); —eluting the bound biomolecules in the elution cell.

公开(公告)号：US11686726B2

公开(公告)日：2023-06-27

申请号：US16470607

申请日：2017-12-27

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Ola Lind ,  Johan Fredrik Avallin ,  Andreas Torbjorn Lundin ,  Nils Norrman ,  Ronnie Palmgren ,  Gustav Jose Rodrigo

IPC: G01N33/543 ,  C12M3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/34 ,  C12N13/00 ,  B01D21/00 ,  B03C5/00 ,  B03C5/02 ,  B03C1/30 ,  B03C1/28 ,  B03C1/01

CPC classification number: G01N33/54326 ,  B01D21/0009 ,  B03C1/30 ,  B03C5/00 ,  B03C5/02 ,  C12M23/48 ,  C12M27/16 ,  C12M29/00 ,  C12M41/44 ,  C12M45/07 ,  C12M47/04 ,  C12M47/10 ,  C12N13/00 ,  G01N33/5436 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  G01N2333/31

Abstract: A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: —providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; —separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; —forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); —eluting the bound biomolecules in the elution cell.

公开(公告)号：US20160367911A1

公开(公告)日：2016-12-22

申请号：US15173215

申请日：2016-06-03

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Enrique Napoleon CARREDANO ,  Gustav Jose Rodrigo

IPC: B01D15/36 ,  B01D15/16 ,  B01D15/42 ,  B01D15/34

CPC classification number: B01D15/362 ,  B01D15/166 ,  B01D15/1878 ,  B01D15/327 ,  B01D15/34 ,  B01D15/3847 ,  B01D15/424 ,  G01N30/34 ,  G01N30/8658 ,  G01N30/8693 ,  G01N2030/8813

Abstract: The present invention relates to a method for the determination of chromatography conditions for the separation of a biomolecule from a liquid sample, which method comprises selecting a number of experiments using design of experiments (DoE); performing said experiments with in-line conditioning of orthogonal quality measures; and based on the results from the experiments, determining efficient chromatography conditions for said biomolecule.The invention also relates to a system for performing the method as well as a computer program and an instrument comprising such a computer program.

Abstract translation: 本发明还涉及用于执行该方法的系统以及包括这样的计算机程序的计算机程序和仪器。

公开(公告)号：US10376813B2

公开(公告)日：2019-08-13

申请号：US15173215

申请日：2016-06-03

Applicant: GE Healthcare Bio-Sciences AB

Inventor： Enrique Napoleon Carredano ,  Gustav Jose Rodrigo

IPC: B01D15/36 ,  B01D15/16 ,  B01D15/34 ,  B01D15/42

Abstract: The present invention relates to a method for the determination of chromatography conditions for the separation of a biomolecule from a liquid sample, which method comprises selecting a number of experiments using design of experiments (DoE); performing said experiments with in-line conditioning of orthogonal quality measures; and based on the results from the experiments, determining efficient chromatography conditions for said biomolecule.The invention also relates to a system for performing the method as well as a computer program and an instrument comprising such a computer program.