公开(公告)号：US20190185911A1

公开(公告)日：2019-06-20

申请号：US16241778

申请日：2019-01-07

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. NELSON ,  Lyle J. ARNOLD ,  Lizhong DAI ,  Steven PHELPS ,  Jijumon CHELLISERRY

IPC: C12Q1/6806 ,  C12Q1/6865 ,  C12Q1/6825 ,  C12Q1/6848 ,  C12P19/34 ,  C12Q1/70 ,  C12Q1/68

CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6848 ,  C12Q1/6865 ,  C12Q1/70 ,  C12Q2537/101 ,  C12Q2545/10

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

公开(公告)号：US20140231710A1

公开(公告)日：2014-08-21

申请号：US14261263

申请日：2014-04-24

Applicant: Gen-Probe Incorporated

Inventor： Norman C. NELSON ,  Kenneth A. BROWNE ,  Lizhong DAI ,  James RUSSELL ,  Mark E. FILIPOWSKY ,  Margarita B. KAMINSKY ,  Daniel L. KACIAN

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  A61L2/186 ,  C11D3/3956 ,  C12Q1/6848

Abstract: The disclosure relates to formulations for use in deactivating nucleic acids and methods of making and using the same.

Abstract translation: 本公开涉及用于减活核酸的制剂及其制备和使用方法。

公开(公告)号：US20210403982A1

公开(公告)日：2021-12-30

申请号：US17328312

申请日：2021-05-24

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. NELSON ,  Lyle J. ARNOLD, Jr. ,  Lizhong DAI ,  Steven PHELPS ,  Jijumon CHELLISERRY

IPC: C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/70 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6825

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

公开(公告)号：US20140066326A1

公开(公告)日：2014-03-06

申请号：US14014607

申请日：2013-08-30

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. NELSON ,  Lyle J. ARNOLD, JR. ,  Lizhong DAI ,  Steven PHELPS ,  Jijumon CHELLISERRY

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6848 ,  C12Q1/6865 ,  C12Q1/70 ,  C12Q2537/101 ,  C12Q2545/10

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

Abstract translation: 公开了用于核酸扩增的改进方法，包括多重扩增，其中扩增在两个或多个不同阶段中进行。 第一相扩增反应优选缺少指数扩增所需的一种或多种组分。 随后在第二，第三或另外的扩增相中提供缺乏的组分，导致快速指数扩增反应。 多相协议在低分析物浓度下导致更快更灵敏的检测和更低的变异性。 还公开了用于实施所要求保护的方法的组合物。