公开(公告)号：US20150258188A1

公开(公告)日：2015-09-17

申请号：US14727248

申请日：2015-06-01

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Mariagrazia PIZZA ,  Davide SERRUTO ,  Rino RAPPUOLI

IPC: A61K39/095 ,  C12N1/20 ,  C12N13/00

CPC classification number: A61K39/095 ,  A61K2039/55555 ,  C07K14/22 ,  C12N1/06 ,  C12N1/20 ,  C12N13/00

Abstract: Existing methods of meningococcal OMV preparation involve the use of detergent during disruption of the bacterial membrane. According to the invention, membrane disruption is performed substantially in the absence of detergent. The resulting OMVs which retain important bacterial immunogenic components, particularly (i) the protective NspA surface protein, (ü) protein NMB2132 and (iii) protein NMB 1870. A Typical process involves the following steps: (a) treating bacterial cells in the substantial absence of detergent; (b) centrifuging the composition from step (a) to separate the outer membrane vesicles from treated cells and cell debris, and collecting the supernatant; (c) performing a high speed centrifugation of the supernatant from step (b) and collecting the outer membrane vesicles in a pellet; (d) re-dispersing the pellet from step (c) in a buffer; (e) performing a second high speed centrifugation in accordance with step (c), collecting the outer membrane vesicles in a pellet; (f) re-dispersing the pellet from step (e) in an aqueous medium.

Abstract translation: 脑膜炎球菌OMV制剂的现有方法涉及在细菌膜破坏期间使用洗涤剂。 根据本发明，膜破坏基本上在没有洗涤剂的情况下进行。 特别是（i）保护性NspA表面蛋白质（ü）蛋白质NMB2132和（iii）蛋白质NMB1870）的所得OMVs。一个典型的方法包括以下步骤：（a）处理实质上的细菌细胞 不含洗涤剂; （b）将来自步骤（a）的组合物离心以将外膜囊泡与经处理的细胞和细胞碎片分离，并收集上清液; （c）对来自步骤（b）的上清液进行高速离心并将颗粒中的外膜囊泡收集; （d）将来自步骤（c）的沉淀物重新分散在缓冲液中; （e）根据步骤（c）进行第二高速离心，将外膜囊泡收集在颗粒中; （f）将来自步骤（e）的沉淀物重新分散在水性介质中。