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    BACTERIAL OUTER MEMBRANE VESICLES
    1.
    发明申请
    BACTERIAL OUTER MEMBRANE VESICLES 审中-公开
    细菌外膜修复

    公开(公告)号：US20150258188A1

    公开(公告)日：2015-09-17

    申请号：US14727248

    申请日：2015-06-01

    Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

    Inventor： Mariagrazia PIZZA ,  Davide SERRUTO ,  Rino RAPPUOLI

    IPC: A61K39/095 ,  C12N1/20 ,  C12N13/00

    CPC classification number: A61K39/095 ,  A61K2039/55555 ,  C07K14/22 ,  C12N1/06 ,  C12N1/20 ,  C12N13/00

    Abstract: Existing methods of meningococcal OMV preparation involve the use of detergent during disruption of the bacterial membrane. According to the invention, membrane disruption is performed substantially in the absence of detergent. The resulting OMVs which retain important bacterial immunogenic components, particularly (i) the protective NspA surface protein, (ü) protein NMB2132 and (iii) protein NMB 1870. A Typical process involves the following steps: (a) treating bacterial cells in the substantial absence of detergent; (b) centrifuging the composition from step (a) to separate the outer membrane vesicles from treated cells and cell debris, and collecting the supernatant; (c) performing a high speed centrifugation of the supernatant from step (b) and collecting the outer membrane vesicles in a pellet; (d) re-dispersing the pellet from step (c) in a buffer; (e) performing a second high speed centrifugation in accordance with step (c), collecting the outer membrane vesicles in a pellet; (f) re-dispersing the pellet from step (e) in an aqueous medium.

    Abstract translation: 脑膜炎球菌OMV制剂的现有方法涉及在细菌膜破坏期间使用洗涤剂。 根据本发明，膜破坏基本上在没有洗涤剂的情况下进行。 特别是（i）保护性NspA表面蛋白质（ü）蛋白质NMB2132和（iii）蛋白质NMB1870）的所得OMVs。一个典型的方法包括以下步骤：（a）处理实质上的细菌细胞 不含洗涤剂; （b）将来自步骤（a）的组合物离心以将外膜囊泡与经处理的细胞和细胞碎片分离，并收集上清液; （c）对来自步骤（b）的上清液进行高速离心并将颗粒中的外膜囊泡收集; （d）将来自步骤（c）的沉淀物重新分散在缓冲液中; （e）根据步骤（c）进行第二高速离心，将外膜囊泡收集在颗粒中; （f）将来自步骤（e）的沉淀物重新分散在水性介质中。

    NEISSERIAL ANTIGENIC PEPTIDES
    3.
    发明申请
    NEISSERIAL ANTIGENIC PEPTIDES 审中-公开
    NEISSERIAL抗原肽

    公开(公告)号：US20160051658A1

    公开(公告)日：2016-02-25

    申请号：US14750837

    申请日：2015-06-25

    Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

    Inventor： Cesira GALEOTTI ,  Guido GRANDI ,  Vega MASIGNANI ,  Marirosa MORA ,  Mariagrazia PIZZA ,  Rino RAPPUOLI ,  Giulio RATTI ,  Vincenzo SCARLATO ,  Maria SCARSELLI

    IPC: A61K39/095

    CPC classification number: A61K39/095 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/55505 ,  C07K14/22

    Abstract: This invention provides, among other things, proteins, polypeptides, and fragments thereof, derived from the bacteria Neisseria meningitidis B. Also provided are nucleic acids encoding for such proteins, polypeptides, and/or fragments, as well as nucleic acids complementary thereto e.g., antisense nucleic acids). Additionally, this invention provides antibodies which bind to the proteins, polypeptides, and/or fragments. This invention further provides expression vectors useful for making the proteins, polypeptides, and/or fragments, as well as host cells transformed with such vectors. This invention also provides compositions of the proteins, polypeptides, fragments, and/or nucleic acids, for use as vaccines, diagnostic reagents, immunogenic compositions, and the like. Methods of making the compositions and methods of treatment with the compositions are also provided. This invention also provides methods of detecting the proteins, polypeptides, fragments, and/or nucleic acids.

    Abstract translation: 本发明尤其提供衍生自脑膜炎奈瑟氏球菌B细菌的蛋白质，多肽和其片段。还提供编码这种蛋白质，多肽和/或片段的核酸，以及与其互补的核酸，例如， 反义核酸）。 另外，本发明提供了结合蛋白质，多肽和/或片段的抗体。 本发明进一步提供了可用于制备蛋白质，多肽和/或片段以及用这些载体转化的宿主细胞的表达载体。 本发明还提供用作疫苗，诊断试剂，免疫原性组合物等的蛋白质，多肽，片段和/或核酸的组合物。 还提供了用组合物制备组合物和处理方法的方法。 本发明还提供了检测蛋白质，多肽，片段和/或核酸的方法。

    OUTER MEMBRANE VESICLE (OMV) VACCINE COMPRISING N. MENINGITIDIS SEROGROUP B OUTER MEMBRANE PROTEINS
    6.
    发明申请
    OUTER MEMBRANE VESICLE (OMV) VACCINE COMPRISING N. MENINGITIDIS SEROGROUP B OUTER MEMBRANE PROTEINS 审中-公开
    外膜蛋白（OMV）包含N. MENINGITIDIS SEROGROUP B外膜蛋白的疫苗

    公开(公告)号：US20160082098A1

    公开(公告)日：2016-03-24

    申请号：US14961725

    申请日：2015-12-07

    Applicant: GlaxoSmithKline Biologicals SA

    Inventor： Mariagrazia PIZZA ,  Rino RAPPUOLI ,  Marzia Monica GIULIANI

    IPC: A61K39/095

    CPC classification number: A61K39/095 ,  A61K2039/54 ,  A61K2039/55505 ,  A61K2039/6068 ,  A61K2039/70 ,  Y02A50/466

    Abstract: A composition comprising (a) Neisseria meningitidis serogroup B outer membrane vesicles (OMVs), and (b) an immunogenic component selected from other Neisseria proteins, or immunogenic fragments thereof. Component (b) preferably includes a protein from a different NmB strain from that from which the OMV of component (a) is derived. The OMVs are preferably obtained by deoxycholate extraction. Optionally, the composition may also comprise a protective antigen against other pathogens.

    Abstract translation: 一种组合物，其包含（a）脑膜炎奈瑟氏球菌血清群B外膜囊泡（OMV））和（b）选自其他奈瑟氏球菌蛋白质的免疫原性成分或其免疫原性片段。 组分（b）优选包括来自与组分（a）的OMV得自其的不同NmB应变的蛋白质。 OMV优选通过脱氧胆酸提取获得。 任选地，组合物还可以包含抗其他病原体的保护性抗原。

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