公开(公告)号：US11959092B2

公开(公告)日：2024-04-16

申请号：US16635352

申请日：2018-08-07

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Amirreza Faridmoayer ,  Michael Thomas Kowarik ,  Gerd Martin Lipowsky ,  Fabio Serventi

IPC: C12N9/00 ,  C12N9/10 ,  C12N15/90

CPC classification number: C12N15/902 ,  C12N9/1048 ,  C12N9/1051 ,  C12N2800/24 ,  C12N2800/30

Abstract: The present invention discloses a process for engineering a host cell comprising the steps of; a) integrating a first polynucleotide cassette including a first selection marker flanked by a first pair of recombination sites; b) removing the first selection marker by the action of a recombinase which recognises the first pair of recombination sites; c) integrating a second polynucleotide cassette including a second selection marker flanked by a second pair of recombination sites; and d) removing the second selection marker by the action of a recombinase which recognises the second pair of recombination sites. Also disclosed is a host cell genome polynucleotide comprising a first recombinantly engineered region and a second recombinantly engineered region, wherein a first single recombination site is adjacent to the first recombinantly engineered region, and a second single recombination site is adjacent to the second recombinantly engineered region.

公开(公告)号：US11654190B2

公开(公告)日：2023-05-23

申请号：US16620911

申请日：2018-07-17

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Amirreza Faridmoayer ,  Rainer Follador ,  Stefan Jochen Kemmler ,  Michael Thomas Kowarik ,  Gerd Martin Lipowsky ,  Gerald Johann Posch ,  Fabio Serventi

IPC: A61K39/09 ,  C12P19/04 ,  C12P21/00

CPC classification number: A61K39/092 ,  C12P19/04 ,  C12P21/005

Abstract: The disclosure provides synthetic (e.g. recombinant) pneumococcal saccharide comprising one or more repeat unit(s) →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→. Also provided are conjugates comprising a →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→, immunogenic compositions, vaccines and their use in preventing or treating infection by Streptococcus pneumoniae.