公开(公告)号：US12128096B2

公开(公告)日：2024-10-29

申请号：US17804679

申请日：2022-05-31

申请人： GLAXOSMITHKLINE BIOLOGICALS SA

发明人： Amirreza Faridmoayer ,  Rainer Follador ,  Stefan Jochen Kemmler ,  Michael Thomas Kowarik ,  Gerald Johann Posch ,  Fabio Serventi

IPC分类号： A61K39/09 ,  A61K39/102 ,  C08B37/00 ,  A61K35/74 ,  A61K38/00 ,  A61K47/36 ,  B01J20/32 ,  C07K9/00 ,  C07K14/315

CPC分类号： A61K39/092 ,  A61K39/102 ,  C08B37/006 ,  A61K35/74 ,  A61K38/00 ,  A61K47/36 ,  B01J20/32 ,  C07K9/005 ,  C07K14/315

摘要： The disclosure provides synthetic (e.g. recombinant) pneumococcal saccharides comprising one or more repeat unit(s) →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→. Also provided are conjugates comprising a →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→, immunogenic compositions, vaccines and their use in preventing or treating infection by Streptococcus pneumoniae.

公开(公告)号：US11959092B2

公开(公告)日：2024-04-16

申请号：US16635352

申请日：2018-08-07

申请人： GLAXOSMITHKLINE BIOLOGICALS SA

发明人： Amirreza Faridmoayer ,  Michael Thomas Kowarik ,  Gerd Martin Lipowsky ,  Fabio Serventi

IPC分类号： C12N9/00 ,  C12N9/10 ,  C12N15/90

CPC分类号： C12N15/902 ,  C12N9/1048 ,  C12N9/1051 ,  C12N2800/24 ,  C12N2800/30

摘要： The present invention discloses a process for engineering a host cell comprising the steps of; a) integrating a first polynucleotide cassette including a first selection marker flanked by a first pair of recombination sites; b) removing the first selection marker by the action of a recombinase which recognises the first pair of recombination sites; c) integrating a second polynucleotide cassette including a second selection marker flanked by a second pair of recombination sites; and d) removing the second selection marker by the action of a recombinase which recognises the second pair of recombination sites. Also disclosed is a host cell genome polynucleotide comprising a first recombinantly engineered region and a second recombinantly engineered region, wherein a first single recombination site is adjacent to the first recombinantly engineered region, and a second single recombination site is adjacent to the second recombinantly engineered region.

公开(公告)号：US11654190B2

公开(公告)日：2023-05-23

申请号：US16620911

申请日：2018-07-17

申请人： GLAXOSMITHKLINE BIOLOGICALS SA

发明人： Amirreza Faridmoayer ,  Rainer Follador ,  Stefan Jochen Kemmler ,  Michael Thomas Kowarik ,  Gerd Martin Lipowsky ,  Gerald Johann Posch ,  Fabio Serventi

IPC分类号： A61K39/09 ,  C12P19/04 ,  C12P21/00

CPC分类号： A61K39/092 ,  C12P19/04 ,  C12P21/005

摘要： The disclosure provides synthetic (e.g. recombinant) pneumococcal saccharide comprising one or more repeat unit(s) →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→. Also provided are conjugates comprising a →4)-β-D-Glcp-(1→3)-[[α-L-Rhap-(1→2)]-[Gro-(2→P→3)]-β-D-Galp-(1→4)]-β-L-Rhap-(1→, immunogenic compositions, vaccines and their use in preventing or treating infection by Streptococcus pneumoniae.

公开(公告)号：US11819544B2

公开(公告)日：2023-11-21

申请号：US16954337

申请日：2018-12-19

申请人： GLAXOSMITHKLINE BIOLOGICALS SA

发明人： Amirreza Faridmoayer ,  Sabina Marietta Gerber ,  Stefan Jochen Kemmler

IPC分类号： A61K39/085 ,  A61K47/64 ,  A61K39/00 ,  C07K14/31

CPC分类号： A61K39/085 ,  A61K47/646 ,  C07K14/31 ,  A61K2039/6068

摘要： The present invention discloses modified Staphylococcus aureus ClfA proteins that contain glycosylation site consensus sequences. The invention also discloses a conjugate comprising a modified ClfA protein and an antigen (for example a Staphylococcus aureus saccharide antigen), wherein the antigen is linked (either directly or through a linker) to an amino acid residue of the modified ClfA protein.

公开(公告)号：US11286283B2

公开(公告)日：2022-03-29

申请号：US16954306

申请日：2018-12-19

申请人： GLAXOSMITHKLINE BIOLOGICALS SA

发明人： Martin Braun ,  Amirreza Faridmoayer ,  Sabina Marietta Gerber ,  Markus Mueller

IPC分类号： C07K1/00 ,  C07K14/31 ,  A61K47/64 ,  A61K39/085 ,  C12N9/10 ,  A61K38/00

摘要： The present invention discloses modified Staphylococcus aureus HIa proteins which show reduced tendency to aggregate, improving protein stability and yield. Said modified HIa proteins optionally also contain glycosylation site consensus sequences. The invention also discloses a conjugate comprising a modified HIa protein and an antigen (for example a Staphylococcus aureus saccharide antigen), wherein the antigen is linked to an amino acid residue of the modified HIa protein.

公开(公告)号：US11376314B2

公开(公告)日：2022-07-05

申请号：US16620921

申请日：2018-07-17

申请人： GLAXOSMITHKLINE BIOLOGICALS SA

发明人： Amirreza Faridmoayer ,  Rainer Follador ,  Stefan Jochen Kemmler ,  Michael Thomas Kowarik ,  Gerald Johann Posch ,  Fabio Serventi

IPC分类号： A61K39/09 ,  C08B37/00 ,  A61K39/102 ,  A61K38/00 ,  C07K14/315 ,  A61K35/74 ,  A61K47/36 ,  C07K9/00 ,  B01J20/32

摘要： The disclosure provides synthetic (e.g. recombinant) pneumococcal saccharides comprising one or more repeat unit(s) →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→. Also provided are conjugates comprising a →4)-β-D-Glcp-(1→4)-[Gro-(2→P→3)]-β-D-Galp-(1→4)-β-L-Rhap-(1→, immunogenic compositions, vaccines and their use in preventing or treating infection by Streptococcus pneumoniae.

公开(公告)号：US10973901B2

公开(公告)日：2021-04-13

申请号：US16034906

申请日：2018-07-13

申请人： GLAXOSMITHKLINE BIOLOGICALS SA

发明人： Michael Wacker ,  Michael Kowarik ,  Michael Wetter ,  Amirreza Faridmoayer

IPC分类号： A61K39/085 ,  A61K39/09 ,  C12N15/70 ,  C12P21/00 ,  A61K39/00

摘要： Provided herein are prokaryotic cells proficient to produce glycoconjugates in vivo, as well as methods for generating these cell and methods of using these cells to produce glycoconjugates. The compositions of the mentioned glycoconjugates as well as their different uses are also included.