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    PROCESS FOR THE MANIPULATION OF NUCLEIC ACIDS
    3.
    发明公开
    PROCESS FOR THE MANIPULATION OF NUCLEIC ACIDS 审中-公开

    公开(公告)号：US20240279686A1

    公开(公告)日：2024-08-22

    申请号：US18605166

    申请日：2024-03-14

    申请人： GLAXOSMITHKLINE BIOLOGICALS SA

    发明人： Amirreza FARIDMOAYER ,  Michael Thomas KOWARIK ,  Gerd Martin LIPOWSKY ,  Fabio SERVENTI

    IPC分类号： C12N15/90 ,  C12N9/10

    CPC分类号： C12N15/902 ,  C12N9/1048 ,  C12N9/1051 ,  C12N2800/24 ,  C12N2800/30

    摘要： A process for engineering a host cell comprising the steps of a) integrating a first polynucleotide cassette including a first selection marker flanked by a first pair of recombination sites; b) removing the first selection marker by the action of a recombinase which recognises the first pair of recombination sites; c) integrating a second polynucleotide cassette including a second selection marker flanked by a second pair of recombination sites; and d) removing the second selection marker by the action of a recombinase which recognises the second pair of recombination sites. The first and second pairs of recombination sites have identical nucleic acid sequences within each pair, sharing 90-98% nucleic acid sequence identity. Also disclosed is a host cell genome polynucleotide comprising two recombinantly engineered regions, each adjacent to a single recombination site sharing 90-98% identity with each other and any additional recombination sites present in the host cell genome polynucleotide.

    Polypurine tract modified retroviral vectors
    10.
    发明授权
    Polypurine tract modified retroviral vectors 有权

    公开(公告)号：US09796987B2

    公开(公告)日：2017-10-24

    申请号：US12747076

    申请日：2008-12-11

    申请人： Tal Kafri

    发明人： Tal Kafri

    IPC分类号： C12P19/34 ,  C12N15/86 ,  C12N15/63 ,  C12N15/87 ,  C12N5/10 ,  C12N7/01

    CPC分类号： C12N15/86 ,  C12N2740/15043 ,  C12N2740/16043 ,  C12N2800/108 ,  C12N2800/24

    摘要： An integration-defective retroviral vector transfer cassette lacking a functional polypurine tract (PPT) is provided. Also provided are isolated nucleic acids that include a heterologous nucleotide sequence, one or two retroviral long terminal repeats (LTRs), a packaging signal, a rev responsive element, and a eukaryotic promoter, wherein the nucleic acid lacks a functional PPT; vectors that include the disclosed isolated nucleic acids; recombinant retroviral particles and mRNAs thereof; retroviral vector kits; and methods for producing integration-defective vector particles, achieving gene expression of a nucleotide sequence of interest, and inserting a nucleotide sequence of interest into a host cell genome in a site-specific or non-specific manner.