公开(公告)号：US08796505B2

公开(公告)日：2014-08-05

申请号：US12849258

申请日：2010-08-03

申请人： Gabriel Iturriaga de la Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  Jose Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

发明人： Gabriel Iturriaga de la Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  Jose Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

IPC分类号： C12N15/82 ,  C12N15/10 ,  C12N15/00 ,  A01H5/00

CPC分类号： C12N9/2402 ,  C07K2319/00 ,  C12N9/1051 ,  C12N9/16 ,  C12N15/8245 ,  C12Q1/54

摘要： A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5′ terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5′ terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

摘要翻译： 一种用于制备例如选自植物，动物和真菌的真核生物的方法，显示特异性修饰的TPS基因的组成型，诱导型和/或器官特异性表达，其包括提供TPS基因的步骤; 通过将基因与酵母的相应基因对齐，并确定该基因的哪个部分延伸超出酵母基因的5'末端，从而设计适合于TPS基因的修饰; 删除或失活延伸超过酵母基因5'末端的TPS基因的N-末端区域的一部分，以获得增加的海藻糖-6-磷酸合酶活性; 将这样修饰的基因在组成型，诱导型和/或器官特异性启动子的控制下克隆成表达载体; 用如此获得的表达载体转化植物细胞或组织; 并从转化的植物细胞或组织再生完整植物。

公开(公告)号：US07781646B2

公开(公告)日：2010-08-24

申请号：US11065184

申请日：2005-02-24

申请人： Gabriel Iturriaga De La Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  José Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

发明人： Gabriel Iturriaga De La Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  José Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

IPC分类号： C12N15/82 ,  C12N15/63 ,  C12N15/00 ,  A01H5/00

CPC分类号： C12N9/2402 ,  C07K2319/00 ,  C12N9/1051 ,  C12N9/16 ,  C12N15/8245 ,  C12Q1/54

摘要： A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5′ terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5′ terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

摘要翻译： 一种用于制备例如选自植物，动物和真菌的真核生物的方法，显示特异性修饰的TPS基因的组成型，诱导型和/或器官特异性表达，其包括提供TPS基因的步骤; 通过将基因与酵母的相应基因对齐，并确定该基因的哪个部分延伸超出酵母基因的5'末端，从而设计适合于TPS基因的修饰; 删除或失活延伸超过酵母基因5'末端的TPS基因的N-末端区域的一部分，以获得增加的海藻糖-6-磷酸合酶活性; 将这样修饰的基因在组成型，诱导型和/或器官特异性启动子的控制下克隆成表达载体; 用如此获得的表达载体转化植物细胞或组织; 并从转化的植物细胞或组织再生完整植物。

公开(公告)号：US20110296554A1

公开(公告)日：2011-12-01

申请号：US12849258

申请日：2010-08-03

申请人： Gabriel Iturriaga de la Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  José Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

发明人： Gabriel Iturriaga de la Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  José Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

IPC分类号： A01H1/06 ,  C12N15/63 ,  C12N1/15 ,  C12Q1/44 ,  C12N15/74

CPC分类号： C12N9/2402 ,  C07K2319/00 ,  C12N9/1051 ,  C12N9/16 ,  C12N15/8245 ,  C12Q1/54

摘要： A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5′ terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5′ terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

摘要翻译： 一种用于制备例如选自植物，动物和真菌的真核生物的方法，显示特异性修饰的TPS基因的组成型，诱导型和/或器官特异性表达，其包括提供TPS基因的步骤; 通过将基因与酵母的相应基因对齐，并确定该基因的哪个部分延伸超出酵母基因的5'末端，从而设计适合于TPS基因的修饰; 删除或失活延伸超过酵母基因5'末端的TPS基因的N-末端区域的一部分，以获得增加的海藻糖-6-磷酸合酶活性; 将这样修饰的基因在组成型，诱导型和/或器官特异性启动子的控制下克隆成表达载体; 用如此获得的表达载体转化植物细胞或组织; 并从转化的植物细胞或组织再生完整植物。

公开(公告)号：US06872870B1

公开(公告)日：2005-03-29

申请号：US10110502

申请日：1999-10-15

申请人： Gabriel Iturriaga de la Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  José Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

发明人： Gabriel Iturriaga de la Fuente ,  Johan M. Thevelein ,  Patrick Van Dijck ,  José Oscar Mascorro-Gallardo ,  Christophe Van Vaeck

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12N9/10 ,  C12N9/16 ,  C12N15/09 ,  C12N15/29 ,  C12N15/54 ,  C12N15/55 ,  C12N15/62 ,  C12N15/82 ,  C12Q1/25 ,  C12P19/04

CPC分类号： C12N9/2402 ,  C07K2319/00 ,  C12N9/1051 ,  C12N9/16 ,  C12N15/8245 ,  C12Q1/54

摘要： A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5′ terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5′ terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

摘要翻译： 一种用于制备例如选自植物，动物和真菌的真核生物的方法，显示特异性修饰的TPS基因的组成型，诱导型和/或器官特异性表达，其包括提供TPS基因的步骤; 通过将基因与酵母的相应基因对齐，并确定该基因的哪个部分延伸超出酵母基因的5'末端，从而设计适合于TPS基因的修饰; 删除或失活延伸超过酵母基因5'末端的TPS基因的N-末端区域的一部分，以获得增加的海藻糖-6-磷酸合酶活性; 将这样修饰的基因在组成型，诱导型和/或器官特异性启动子的控制下克隆成表达载体; 用如此获得的表达载体转化植物细胞或组织; 并从转化的植物细胞或组织再生完整植物。