公开(公告)号：US10604811B2

公开(公告)日：2020-03-31

申请号：US15841526

申请日：2017-12-14

Applicant: Gen-Probe Incorporated

Inventor： Scott Higgins ,  Mike Webb

IPC: C12Q1/6886 ,  C12Q1/6848 ,  C12N15/00

Abstract: Methods, kits, and compositions for evaluating the quality of nucleic acids within a biological sample for analysis in a molecular assay are provided.

公开(公告)号：US09863004B2

公开(公告)日：2018-01-09

申请号：US14356368

申请日：2012-11-02

Applicant: Gen-Probe Incorporated

Inventor： Scott Higgins ,  Mike Webb

IPC: C12Q1/68 ,  C07H21/02

CPC classification number: C12Q1/6886 ,  C12Q1/6848 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2545/101

Abstract: Methods, kits, and compositions for evaluating the quality of nucleic acids within a biological sample for analysis in a molecular assay are provided. A kit is provided for evaluating a biological sample containing potentially degraded DNA, comprising a control reagent and a target amplification reagent, wherein the control amplification reagent comprises one or more pairs of amplification oligonucleotides capable of amplifying a medium chain acyl-coenzyme A dehydrogenase (MCAD) nucleic acid molecule or a complement thereof, and wherein the target amplification reagent comprises one or more pairs of amplification oligonucleotides capable of amplifying a non-MCAD target gene of interest or a complement thereof. Frequently, one or more of the amplification oligonucleotides is/are labeled.

公开(公告)号：US11685957B2

公开(公告)日：2023-06-27

申请号：US17199683

申请日：2021-03-12

Applicant: Gen-Probe Incorporated

Inventor： Scott Higgins ,  Mike Webb

IPC: C12Q1/6886 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6851

CPC classification number: C12Q1/6886 ,  C12Q1/686 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q2545/101 ,  C12Q2545/114 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q1/6848 ,  C12Q2545/101

Abstract: Methods, kits, and compositions for evaluating the quality of nucleic acids within a biological sample for analysis in a molecular assay are provided.

公开(公告)号：US10995375B2

公开(公告)日：2021-05-04

申请号：US16785837

申请日：2020-02-10

Applicant: Gen-Probe Incorporated

Inventor： Scott Higgins ,  Mike Webb

IPC: C12Q1/6886 ,  C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6848

Abstract: Methods, kits, and compositions for evaluating the quality of nucleic acids within a biological sample for analysis in a molecular assay are provided.

公开(公告)号：US20150225797A1

公开(公告)日：2015-08-13

申请号：US14356368

申请日：2012-11-02

Applicant: Gen-Probe Incorporated

Inventor： Scott Higgins ,  Mike Webb

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/6848 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2545/101

Abstract: Methods, kits, and compositions for evaluating the quality of nucleic acids within a biological sample for analysis in a molecular assay are provided. A kit is provided for evaluating a biological sample containing potentially degraded DNA, comprising a control reagent and a target amplification reagent, wherein the control amplification reagent comprises one or more pairs of amplification oligonucleotides capable of amplifying a medium chain acyl-coenzyme A dehydrogenase (MCAD) nucleic acid molecule or a complement thereof, and wherein the target amplification reagent comprises one or more pairs of amplification oligonucleotides capable of amplifying a non-MCAD target gene of interest or a complement thereof. Frequently, one or more of the amplification oligonucleotides is/are labeled.

Abstract translation: 提供了用于评估生物样品中核酸质量的方法，试剂盒和组合物，用于分子测定中的分析。 提供了一种试剂盒，用于评价含有潜在降解DNA的生物样品，其包含对照试剂和靶扩增试剂，其中对照扩增试剂包含一对或多对能够扩增中链酰基辅酶A脱氢酶（MCAD ）核酸分子或其互补物，并且其中所述靶扩增试剂包含能够扩增目的非MCAD靶基因或其补体的一对或多对扩增寡核苷酸。 通常，一个或多个扩增寡核苷酸被标记。