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    MOLECULAR ASSAY REAGENTS AND METHODS
    2.
    发明申请
    MOLECULAR ASSAY REAGENTS AND METHODS 有权
    分子测定试剂和方法

    公开(公告)号：US20150225797A1

    公开(公告)日：2015-08-13

    申请号：US14356368

    申请日：2012-11-02

    Applicant: Gen-Probe Incorporated

    Inventor： Scott Higgins ,  Mike Webb

    IPC: C12Q1/68

    CPC classification number: C12Q1/6886 ,  C12Q1/6848 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2545/101

    Abstract: Methods, kits, and compositions for evaluating the quality of nucleic acids within a biological sample for analysis in a molecular assay are provided. A kit is provided for evaluating a biological sample containing potentially degraded DNA, comprising a control reagent and a target amplification reagent, wherein the control amplification reagent comprises one or more pairs of amplification oligonucleotides capable of amplifying a medium chain acyl-coenzyme A dehydrogenase (MCAD) nucleic acid molecule or a complement thereof, and wherein the target amplification reagent comprises one or more pairs of amplification oligonucleotides capable of amplifying a non-MCAD target gene of interest or a complement thereof. Frequently, one or more of the amplification oligonucleotides is/are labeled.

    Abstract translation: 提供了用于评估生物样品中核酸质量的方法，试剂盒和组合物，用于分子测定中的分析。 提供了一种试剂盒，用于评价含有潜在降解DNA的生物样品，其包含对照试剂和靶扩增试剂，其中对照扩增试剂包含一对或多对能够扩增中链酰基辅酶A脱氢酶（MCAD ）核酸分子或其互补物，并且其中所述靶扩增试剂包含能够扩增目的非MCAD靶基因或其补体的一对或多对扩增寡核苷酸。 通常，一个或多个扩增寡核苷酸被标记。

    Molecular assay reagents and methods
    3.
    发明授权
    Molecular assay reagents and methods 有权

    公开(公告)号：US09863004B2

    公开(公告)日：2018-01-09

    申请号：US14356368

    申请日：2012-11-02

    Applicant: Gen-Probe Incorporated

    Inventor： Scott Higgins ,  Mike Webb

    IPC: C12Q1/68 ,  C07H21/02

    CPC classification number: C12Q1/6886 ,  C12Q1/6848 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2545/101

    Abstract: Methods, kits, and compositions for evaluating the quality of nucleic acids within a biological sample for analysis in a molecular assay are provided. A kit is provided for evaluating a biological sample containing potentially degraded DNA, comprising a control reagent and a target amplification reagent, wherein the control amplification reagent comprises one or more pairs of amplification oligonucleotides capable of amplifying a medium chain acyl-coenzyme A dehydrogenase (MCAD) nucleic acid molecule or a complement thereof, and wherein the target amplification reagent comprises one or more pairs of amplification oligonucleotides capable of amplifying a non-MCAD target gene of interest or a complement thereof. Frequently, one or more of the amplification oligonucleotides is/are labeled.

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