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公开(公告)号：US20220308042A1
公开(公告)日：2022-09-29
申请号：US17842528
申请日：2022-06-16
Applicant: Genzyme Corporation
Inventor： Jonathan Wang , Neha Shah , Jason Walther , Jiuyi Lu , Timothy Johnson , Yukun Ren , Jean McLarty
Abstract: Provided herein are methods of predicting the effect of a concentration of a sensitizer on cell viability in a production bioreactor, methods of improving cell viability in a production bioreactor, methods of predicting cell viability in a production bioreactor, and methods for culturing a cell in a production bioreactor.
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公开(公告)号：US20190285617A1
公开(公告)日：2019-09-19
申请号：US16355387
申请日：2019-03-15
Applicant: Genzyme Corporation
Inventor： Jonathan Wang , Neha Shah , Jason Walther , Jiuyi Lu , Timothy Johnson , Yukun Ren , Jean McLarty
Abstract: Provided herein are methods of predicting the effect of a concentration of a sensitizer on cell viability in a production bioreactor, methods of improving cell viability in a production bioreactor, methods of predicting cell viability in a production bioreactor, and methods for culturing a cell in a production bioreactor.
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公开(公告)号：US20250027031A1
公开(公告)日：2025-01-23
申请号：US18823894
申请日：2024-09-04
Applicant: Genzyme Corporation
Inventor： Jiuyi Lu , Jason Walther , Jonathan Wang , Kevin Victor Chen
Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.
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公开(公告)号：US12157873B2
公开(公告)日：2024-12-03
申请号：US18374435
申请日：2023-09-28
Applicant: Genzyme Corporation
Inventor： Kevin Brower , Michael Coolbaugh , Xhorxhi Gjoka , Chad Varner , Jason Walther
Abstract: Systems and devices for producing biological products and methods of using the same are provided. An example universal skid includes a rigid support and a plurality of flow-channel hardware sets. Each flow-channel hardware set couples with a respective flow channel of a plurality of flow channels for unit operations. The flow-channel hardware sets allow the flow channels to operate in parallel or in series for continuous processing over the unit operations. An example modular assembly includes one or more single-use kits for one or more unit operations and a universal skid coupled with the one or more single-use kits. An example manufacturing system includes multiple modular assemblies coupled with each other allowing continuous processing within each modular assembly, in between the modular assemblies, fully continuous processing across multiple unit operations.
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公开(公告)号：US12110483B2
公开(公告)日：2024-10-08
申请号：US18308870
申请日：2023-04-28
Applicant: Genzyme Corporation
Inventor： Jiuyi Lu , Jason Walther , Jonathan Wang , Kevin Victor Chen
CPC classification number: C12M41/48 , B01D15/1807 , B01D61/18 , B01D61/22 , C12M29/04 , C12M29/10 , C12M41/40 , B01D2311/2626 , B01D2311/2688 , B01D2313/243 , B01D2315/10 , B01D2317/04 , B01D2317/06
Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.
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Patent Agency Ranking
公开(公告)号：US11306341B2
公开(公告)日：2022-04-19
申请号：US14976486
申请日：2015-12-21
Applicant: Genzyme Corporation
Inventor： Christopher Hwang , Timothy Johnson , Jason Walther , Cheng Cheng , Jonathan Wang , Neha Shah , Seul-A Bae
Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.
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公开(公告)号：US11434464B2
公开(公告)日：2022-09-06
申请号：US17524269
申请日：2021-11-11
Applicant: Genzyme Corporation
Inventor： Jiuyi Lu , Jason Walther , Jonathan Wang , Kevin Victor Chen
Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.
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公开(公告)号：US20220145348A1
公开(公告)日：2022-05-12
申请号：US17572978
申请日：2022-01-11
Applicant: Genzyme Corporation
Inventor： Christopher Hwang , Timothy Johnson , Jason Walther , Cheng Cheng , Jonathan Wang , Neha Shah , Seul-A Bae
Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.
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公开(公告)号：US20220073861A1
公开(公告)日：2022-03-10
申请号：US17524269
申请日：2021-11-11
Applicant: Genzyme Corporation
Inventor： Jiuyi Lu , Jason Walther , Jonathan Wang , Kevin Victor Chen
Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.
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公开(公告)号：US20250051705A1
公开(公告)日：2025-02-13
申请号：US18930067
申请日：2024-10-29
Applicant: Genzyme Corporation
Inventor： Kevin Brower , Michael Coolbaugh , Xhorxhi Gjoka , Chad Varner , Jason Walther
Abstract: Systems and devices for producing biological products and methods of using the same are provided. An example universal skid includes a rigid support and a plurality of flow-channel hardware sets. Each flow-channel hardware set couples with a respective flow channel of a plurality of flow channels for unit operations. The flow-channel hardware sets allow the flow channels to operate in parallel or in series for continuous processing over the unit operations. An example modular assembly includes one or more single-use kits for one or more unit operations and a universal skid coupled with the one or more single-use kits. An example manufacturing system includes multiple modular assemblies coupled with each other allowing continuous processing within each modular assembly, in between the modular assemblies, fully continuous processing across multiple unit operations.
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