公开(公告)号：US20220145348A1

公开(公告)日：2022-05-12

申请号：US17572978

申请日：2022-01-11

Applicant: Genzyme Corporation

Inventor： Christopher Hwang ,  Timothy Johnson ,  Jason Walther ,  Cheng Cheng ,  Jonathan Wang ,  Neha Shah ,  Seul-A Bae

IPC: C12P21/00 ,  C07K16/00 ,  C12N5/00

Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

公开(公告)号：US11306341B2

公开(公告)日：2022-04-19

申请号：US14976486

申请日：2015-12-21

Applicant: Genzyme Corporation

Inventor： Christopher Hwang ,  Timothy Johnson ,  Jason Walther ,  Cheng Cheng ,  Jonathan Wang ,  Neha Shah ,  Seul-A Bae

IPC: C12P21/00 ,  C12N5/00 ,  C07K16/00

Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

公开(公告)号：US20160177361A1

公开(公告)日：2016-06-23

申请号：US14976486

申请日：2015-12-21

Applicant: Genzyme Corporation

Inventor： Christopher Hwang ,  Timothy Johnson ,  Jason Walther ,  Cheng Cheng ,  Jonathan Wang ,  Neha Shah ,  Seul-A Bae

IPC: C12P21/00 ,  C07K16/00

CPC classification number: C12P21/00 ,  C07K16/00 ,  C07K2317/14 ,  C12N5/0043 ,  C12N5/005 ,  C12N2500/50 ,  C12N2510/02

Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

Abstract translation: 本文提供了在液体培养基中培养哺乳动物细胞的方法，所述液体培养基包括浓度为1.8g / L或浓度高于1.8g / L的泊洛沙姆-188或包含选择的泊洛沙姆188浓度的液体培养基 基于选自以下组中的一种或多种因素：孔径，孔型，气体流速，培养基中的活细胞密度和与细胞应激相关的标记。