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公开(公告)号：US08093033B2
公开(公告)日：2012-01-10
申请号：US12336158
申请日：2008-12-16
申请人： George Kemble , Gregory Duke
发明人： George Kemble , Gregory Duke
IPC分类号： C12N7/00 , C12N5/07 , A61K39/145
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K2039/5254 , A61K2039/543 , C12N15/85 , C12N2760/16134 , C12N2760/16151 , C12N2760/16152 , C12N2760/16162 , C12N2760/16234 , C12N2760/16251 , C12N2760/16252 , C12N2760/16261 , C12N2840/20
摘要： Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.
摘要翻译： 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外，本发明提供了生产能够在含胚鸡蛋和/或细胞（例如Vero和/或MDCK）中复制的流感病毒的方法，并进一步提供具有增强的复制特征的流感病毒。 此外，本发明包括一种改进的抢救方法，其中用本发明的质粒和载体对动物细胞（例如，SF Vero细胞）进行电穿孔。
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公开(公告)号：US20070292455A1
公开(公告)日：2007-12-20
申请号：US11614141
申请日：2006-12-21
申请人： George Kemble , Gregory Duke , Richard Spaete
发明人： George Kemble , Gregory Duke , Richard Spaete
IPC分类号： A61K39/245 , A61P37/04 , C12N7/00
CPC分类号： C12N7/00 , C12N2710/16162
摘要： A method is provided for attenuating a cytomegalovirus comprising functionally disrupting an open reading frame of a Toledo genome region or its homolog and making chimeric CMV virus genomes.
摘要翻译： 提供了一种减毒巨细胞病毒的方法，其包括功能性地破坏托莱多基因组区域的开放阅读框或其同源物并制备嵌合CMV病毒基因组。
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3.发明授权Methods and compositions for expressing negative-sense viral RNA in canine cells 失效在犬细胞中表达负义病毒RNA的方法和组合物公开(公告)号：US08278433B2
公开(公告)日：2012-10-02
申请号：US12842854
申请日：2010-07-23
申请人： Gregory Duke , George Kemble , James Young , Zhaoti Wang
发明人： Gregory Duke , George Kemble , James Young , Zhaoti Wang
IPC分类号： C07H21/00 , A61K39/145
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , C12N9/1247 , C12N15/85 , C12N2510/02 , C12N2760/16134 , C12N2760/16234 , C12N2830/008 , C12Q1/70
摘要： The present invention provides novel canine pol I regulatory nucleic acid sequences useful for the expression of nucleic acid sequences in canine cells such as MDCK cells. The invention further provides expression vectors and cells comprising such nucleic acids as well as methods of using such nucleic acids to make influenza viruses, including infectious influenza viruses.
摘要翻译： 本发明提供了可用于在犬细胞如MDCK细胞中表达核酸序列的新型canine pol I调节核酸序列。 本发明进一步提供了包含这种核酸的表达载体和细胞以及使用这种核酸来制造流感病毒（包括感染性流感病毒）的方法。
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4.发明授权Methods and compositions for expressing negative-sense viral RNA in canine cells 有权在犬细胞中表达负义病毒RNA的方法和组合物公开(公告)号：US07790434B2
公开(公告)日：2010-09-07
申请号：US11501067
申请日：2006-08-09
申请人： Gregory Duke , George Kemble , James Young , Zhaoti Wang
发明人： Gregory Duke , George Kemble , James Young , Zhaoti Wang
IPC分类号： C12N7/00 , A61K39/145
CPC分类号： A61K39/145 , A61K39/12 , C12N7/00 , C12N9/1247 , C12N15/85 , C12N2760/16134 , C12N2760/16234 , C12N2830/008 , C12Q1/70
摘要： The present invention provides novel canine pol I regulatory nucleic acid sequences useful for the expression of nucleic acid sequences in canine cells such as MDCK cells. The invention further provides expression vectors and cells comprising such nucleic acids as well as methods of using such nucleic acids to make influenza viruses, including infectious influenza viruses.
摘要翻译： 本发明提供了可用于在犬细胞如MDCK细胞中表达核酸序列的新型canine pol I调节核酸序列。 本发明进一步提供了包含这种核酸的表达载体和细胞以及使用这种核酸来制造流感病毒（包括感染性流感病毒）的方法。
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公开(公告)号：US20090208527A1
公开(公告)日：2009-08-20
申请号：US12336158
申请日：2008-12-16
申请人： George Kemble , Gregory Duke
发明人： George Kemble , Gregory Duke
IPC分类号： A61K39/145 , C12N15/87 , C12N7/01
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K2039/5254 , A61K2039/543 , C12N15/85 , C12N2760/16134 , C12N2760/16151 , C12N2760/16152 , C12N2760/16162 , C12N2760/16234 , C12N2760/16251 , C12N2760/16252 , C12N2760/16261 , C12N2840/20
摘要： Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.
摘要翻译： 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外，本发明提供了生产能够在含胚鸡蛋和/或细胞（例如Vero和/或MDCK）中复制的流感病毒的方法，并进一步提供具有增强的复制特征的流感病毒。 此外，本发明包括一种改进的抢救方法，其中用本发明的质粒和载体对动物细胞（例如，SF Vero细胞）进行电穿孔。
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6.发明申请Recombinant Human Cytomegalovirus And Vaccines Comprising Heterologous Antigens 审中-公开重组人巨细胞病毒和包含异源抗原的疫苗公开(公告)号：US20080044384A1
公开(公告)日：2008-02-21
申请号：US11571081
申请日：2005-06-24
申请人： George Kemble , Harry Greenberg , Gregory Duke
发明人： George Kemble , Harry Greenberg , Gregory Duke
CPC分类号： A61K39/245 , A61K39/12 , A61K2039/5256 , A61K2039/53 , C12N2710/16134 , C12N2710/16143 , C12N2770/24234 , Y02A50/41
摘要： The present invention relates to recombinant HCMV (human cytomegalovirus) expressing a pp65 polypeptide or fragment thereof fused to a heterologous or non-native polypeptide, in particular immunogenic and/or antigenic polypeptides. In particular, the heterologous gene products include antigenic or immunogenic polypeptides from a variety of pathogens, cellular genes, tumor antigens, and viruses. The recombinant viruses may advantageously be used in vaccine formulations including vaccines against a broad range of pathogens and antigens.
摘要翻译： 本发明涉及表达与异源或非天然多肽，特别是免疫原性和/或抗原多肽融合的pp65多肽或其片段的重组HCMV（人巨细胞病毒）。 特别地，异源基因产物包括来自多种病原体，细胞基因，肿瘤抗原和病毒的抗原性或免疫原性多肽。 重组病毒可有利地用于疫苗制剂，包括针对各种病原体和抗原的疫苗。
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7.发明申请Methods and compositions for expressing negative-sense viral RNA in canine cells 审中-公开在犬细胞中表达负义病毒RNA的方法和组合物公开(公告)号：US20060286591A1
公开(公告)日：2006-12-21
申请号：US11455734
申请日：2006-06-20
申请人： Gregory Duke , George Kemble , James Young , Zhaoti Wang
发明人： Gregory Duke , George Kemble , James Young , Zhaoti Wang
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , C12N9/1247 , C12N15/85 , C12N2510/02 , C12N2760/16134 , C12N2760/16234 , C12N2830/008 , C12Q1/70
摘要： The present invention provides novel canine pol I regulatory nucleic acid sequences useful for the expression of nucleic acid sequences in canine cells such as MDCK cells. The invention further provides expression vectors and cells comprising such nucleic acids as well as methods of using such nucleic acids to make influenza viruses, including infectious influenza viruses.
摘要翻译： 本发明提供了可用于在犬细胞如MDCK细胞中表达核酸序列的新型canine pol I调节核酸序列。 本发明进一步提供了包含这种核酸的表达载体和细胞以及使用这种核酸来制造流感病毒（包括感染性流感病毒）的方法。
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公开(公告)号：US08409843B2
公开(公告)日：2013-04-02
申请号：US13309498
申请日：2011-12-01
申请人： George Kemble , Gregory Duke
发明人： George Kemble , Gregory Duke
IPC分类号： C12N7/00 , C12N5/07 , A61K39/145
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K2039/5254 , A61K2039/543 , C12N15/85 , C12N2760/16134 , C12N2760/16151 , C12N2760/16152 , C12N2760/16162 , C12N2760/16234 , C12N2760/16251 , C12N2760/16252 , C12N2760/16261 , C12N2840/20
摘要： Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.
摘要翻译： 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外，本发明提供了生产能够在含胚鸡蛋和/或细胞（例如Vero和/或MDCK）中复制的流感病毒的方法，并进一步提供具有增强的复制特征的流感病毒。 此外，本发明包括一种改进的抢救方法，其中用本发明的质粒和载体对动物细胞（例如，SF Vero细胞）进行电穿孔。
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公开(公告)号：US08114415B2
公开(公告)日：2012-02-14
申请号：US12254131
申请日：2008-10-20
申请人： Erich Hoffmann , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
发明人： Erich Hoffmann , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
IPC分类号： A61K39/145 , A61K39/00 , A61K39/12 , C12P21/04
CPC分类号： C12N15/86 , A61K2039/5254 , C07K14/005 , C12N7/00 , C12N15/85 , C12N2760/16122 , C12N2760/16134 , C12N2760/16143 , C12N2760/16162 , C12N2760/16222 , C12N2760/16234 , C12N2760/16243 , C12N2760/16262 , C12N2840/20
摘要： Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25° C. (and immunogenic compositions comprising the same) is also provided.
摘要翻译： 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外，本发明提供了生产能够在含胚鸡蛋和/或细胞（例如Vero和/或MDCK）中复制的流感病毒的方法，并进一步提供具有增强的复制特征的流感病毒。 还提供了生产在例如25℃下有效复制的冷适应（ca）流感病毒的方法（和包含其的免疫原性组合物）。
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10.发明申请METHODS AND COMPOSITIONS FOR EXPRESSING NEGATIVE-SENSE VIRAL RNA IN CANINE CELLS 失效用于表达阴性细胞中的阴性病毒RNA的方法和组合物公开(公告)号：US20110045586A1
公开(公告)日：2011-02-24
申请号：US12842854
申请日：2010-07-23
申请人： Gregory Duke , George Kemble , James Young , Zhaoti Wang
发明人： Gregory Duke , George Kemble , James Young , Zhaoti Wang
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , C12N9/1247 , C12N15/85 , C12N2510/02 , C12N2760/16134 , C12N2760/16234 , C12N2830/008 , C12Q1/70
摘要： The present invention provides novel canine pol I regulatory nucleic acid sequences useful for the expression of nucleic acid sequences in canine cells such as MDCK cells. The invention further provides expression vectors and cells comprising such nucleic acids as well as methods of using such nucleic acids to make influenza viruses, including infectious influenza viruses.
摘要翻译： 本发明提供了可用于在犬细胞如MDCK细胞中表达核酸序列的新型canine pol I调节核酸序列。 本发明进一步提供了包含这种核酸的表达载体和细胞以及使用这种核酸来制造流感病毒（包括感染性流感病毒）的方法。
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