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    Reagentless and reusable biosensors with tunable differential binding affinities and methods of making
    1.
    发明授权
    Reagentless and reusable biosensors with tunable differential binding affinities and methods of making 有权
    具有可调节差异结合亲和力和制备方法的无菌和可重复使用的生物传感器

    公开(公告)号：US07435386B2

    公开(公告)日：2008-10-14

    申请号：US11094540

    申请日：2005-03-25

    申请人： Igor L. Medintz ,  Ellen R. Goldman ,  George P. Anderson ,  J. Matthew Mauro

    发明人： Igor L. Medintz ,  Ellen R. Goldman ,  George P. Anderson ,  J. Matthew Mauro

    IPC分类号： G01N21/00 ,  G01N21/01 ,  G01N21/64 ,  G01N33/53 ,  G01N33/553

    CPC分类号： C12Q1/6818 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N2021/6441 ,  C12Q2565/607 ,  C12Q2565/101

    摘要： The biosensor comprises a modular biorecognition element and a modular flexible arm element. The biorecognition element and the flexible arm element are each labeled with a signaling element. The flexible arm contains an analog of an analyte of interest that binds with the biorecognition element, bringing the two signaling elements in close proximity, which establishes a baseline fluorescence resonance energy transfer (FRET). When an analyte of interest is provided to the biosensor, the analyte will displace the analyte analog, and with it, the signaling module of the modular flexible arm, causing a measurable change in the FRET signal in a analyte concentration dependent manner. The modularity of different portions of the biosensor allows functional flexibility. The biosensor-operates without additional development reagents, requiring only the presence of analyte or target for function.

    摘要翻译： 生物传感器包括模块化生物识别元件和模块化柔性臂元件。 生物识别元件和柔性臂元件各自用信号元件标记。 柔性臂包含与生物识别元件结合的感兴趣分析物的类似物，使得两个信号元件紧密相邻，这建立了基线荧光共振能量转移（FRET）。 当感兴趣的分析物被提供给生物传感器时，分析物将置换分析物类似物，并且借助于该模块化柔性臂的信号模块，以分析​​物浓度依赖的方式引起FRET信号的可测量的变化。 生物传感器的不同部分的模块化允许功能灵活性。 生物传感器无需额外的开发试剂即可进行操作，仅需要分析物或靶标的功能。

    Reagentless and Reusable Biosensors with Tunable Differential Binding Affinities and Methods of Making
    5.
    发明申请
    Reagentless and Reusable Biosensors with Tunable Differential Binding Affinities and Methods of Making 有权
    具有可调节差异结合亲和力的制剂和可重复使用的生物传感器和制备方法

    公开(公告)号：US20090048123A1

    公开(公告)日：2009-02-19

    申请号：US12103778

    申请日：2008-04-16

    申请人： Igor L. Medintz ,  Ellen R. Goldman ,  George P. Anderson ,  J. Matthew Mauro

    发明人： Igor L. Medintz ,  Ellen R. Goldman ,  George P. Anderson ,  J. Matthew Mauro

    IPC分类号： C40B40/04 ,  G01N21/64 ,  C12M1/00 ,  C40B50/00

    CPC分类号： C12Q1/6818 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N2021/6441 ,  C12Q2565/607 ,  C12Q2565/101

    摘要： The biosensor comprises a modular biorecognition element and a modular flexible arm element. The biorecognition element and the flexible arm element are each labeled with a signaling element. The flexible arm contains an analog of an analyte of interest that binds with the biorecognition element, bringing the two signaling elements in close proximity, which establishes a baseline fluorescence resonance energy transfer (FRET). When an analyte of interest is provided to the biosensor, the analyte will displace the analyte analog, and with it, the signaling module of the modular flexible arm, causing a measurable change in the FRET signal in a analyte concentration dependent manner. The modularity of different portions of the biosensor allows functional flexibility. The biosensor operates without additional development reagents, requiring only the presence of analyte or target for function.

    摘要翻译： 生物传感器包括模块化生物识别元件和模块化柔性臂元件。 生物识别元件和柔性臂元件各自用信号元件标记。 柔性臂包含与生物识别元件结合的感兴趣分析物的类似物，使得两个信号元件紧密相邻，这建立了基线荧光共振能量转移（FRET）。 当感兴趣的分析物被提供给生物传感器时，分析物将置换分析物类似物，并且借助于该模块化柔性臂的信号模块，以分析​​物浓度依赖的方式引起FRET信号的可测量的变化。 生物传感器的不同部分的模块化允许功能灵活性。 生物传感器无需额外的开发试剂即可运行，仅需要分析物或靶标的存在才能发挥作用。

    Attachment of biomolecules to hydrophobic surfaces
    9.
    发明授权
    Attachment of biomolecules to hydrophobic surfaces 失效
    生物分子附着到疏水表面

    公开(公告)号：US06713272B2

    公开(公告)日：2004-03-30

    申请号：US09955023

    申请日：2001-09-19

    申请人： George P. Anderson ,  J. Matthew Mauro

    发明人： George P. Anderson ,  J. Matthew Mauro

    IPC分类号： G01N3353

    CPC分类号： G01N33/54393

    摘要： A recombinant protein that is a fusion protein containing a protein binding domain and a domain that is post-translationally modified to become lipid-tagged. This molecule has both a domain with highly specific binding capabilities and a domain which is very hydrophobic in nature. This type of molecule can be used to coat hydrophobic surfaces such as polystyrene easily and effectively for use in solid phase binding assays.

    摘要翻译： 重组蛋白，其是含有蛋白质结合结构域和翻译后修饰以变成脂质标记的结构域的融合蛋白。 该分子具有具有高度特异性结合能力的结构域和本质上非常疏水的结构域。 这种类型的分子可用于容易且有效地涂覆疏水性表面如聚苯乙烯用于固相结合测定。