公开(公告)号：US20060003453A1

公开(公告)日：2006-01-05

申请号：US11222108

申请日：2005-09-08

申请人： Georges Natsoulis ,  Gary Kurtzman ,  Peter Colosi

发明人： Georges Natsoulis ,  Gary Kurtzman ,  Peter Colosi

IPC分类号： C12N7/02 ,  C12N15/861

CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K2319/00 ,  C12N7/00 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2750/14162 ,  C12N2800/30 ,  C12N2840/20

摘要： The present invention provides methods and compositions for producing high titer preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include AAV helper function systems and host cells. The present invention also includes methods of using AAV helper function vectors that effect the production of only small amounts of the long forms of Rep protein, and rAAV virions produced by such methods.

公开(公告)号：US06365403B1

公开(公告)日：2002-04-02

申请号：US09449581

申请日：1999-11-29

申请人： Georges Natsoulis ,  Gary Kurtzman ,  Peter Colosi

发明人： Georges Natsoulis ,  Gary Kurtzman ,  Peter Colosi

IPC分类号： C12N510

CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K2319/00 ,  C12N7/00 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2750/14162 ,  C12N2800/30 ,  C12N2840/20

摘要： The present invention provides methods and compositions for producing high titer preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include AAV helper function systems and host cells. The present invention also includes methods of using AAV helper function vectors that effect the production of only small amounts of the long forms of Rep protein, and rAAV virions produced by such methods.

公开(公告)号：US6027931A

公开(公告)日：2000-02-22

申请号：US107708

申请日：1998-06-30

申请人： George Natsoulis ,  Peter Colosi ,  Gary Kurtzman

发明人： George Natsoulis ,  Peter Colosi ,  Gary Kurtzman

IPC分类号： C07K14/015 ,  C12N5/10 ,  C12N7/04 ,  C12N15/864 ,  C12N7/01 ,  C12N15/86

CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C07K2319/00 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2750/14162 ,  C12N2800/30 ,  C12N2840/20

摘要： The present invention provides methods and compositions for producing high titer preparations of recombinant AAV ("rAAV") virions. The compositions of the present invention include AAV helper function systems and host cells. The present invention also includes methods of using AAV helper function vectors that effect the production of only small amounts of the long forms of Rep protein, and rAAV virions produced by such methods.

摘要翻译： 本发明提供了用于生产重组AAV（“rAAV”）病毒粒子的高效价制剂的方法和组合物。 本发明的组合物包括AAV辅助功能体系和宿主细胞。 本发明还包括使用影响仅产生少量Rep形式的Rep蛋白的AAV辅助功能载体的方法和通过这些方法产生的rAAV病毒体。

公开(公告)号：US07037713B2

公开(公告)日：2006-05-02

申请号：US10074302

申请日：2002-02-11

申请人： Peter Colosi

发明人： Peter Colosi

IPC分类号： C12P21/06 ,  C12P19/34 ,  C12N15/00 ,  C12N15/09 ,  C12N5/00

CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2750/14162 ,  C12N2800/30 ,  C12N2840/20

摘要： The present invention provides methods and compositions for producing high titer, wild-type-free preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include novel nucleic acids encoding AAV helper functions and AAV helper function vectors. The present invention also includes host cells transfected by the claimed nucleic acids, methods of using the claimed vectors, and rAAV virions produced by such methods.

公开(公告)号：US20070026521A1

公开(公告)日：2007-02-01

申请号：US11580801

申请日：2006-10-13

申请人： Peter Colosi

发明人： Peter Colosi

IPC分类号： C12N15/861 ,  C12N5/06 ,  C07H21/04

CPC分类号： C12N7/00 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10344 ,  C12N2710/10352 ,  C12N2710/16222 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2750/14152 ,  C12N2800/108 ,  C12N2830/002 ,  C12N2830/75 ,  C12N2840/20 ,  C12N2840/203

摘要： Accessory functions capable of supporting efficient recombinant AAV (rAAV) virion production in a suitable host cell are provided. The accessory functions are in the form of one or more vectors that are capable of being transferred between cells. Methods of producing rAAV virions are also provided. The methods can be practiced to produce commercially significant levels of rAAV particles without also generating significant levels of infectious helper virus or other contaminating by-products.

摘要翻译： 提供了能够在合适的宿主细胞中支持有效重组AAV（rAAV）病毒粒子产生的辅助功能。 附件功能是能够在单元之间转移的一个或多个向量的形式。 还提供了产生rAAV病毒粒子的方法。 可以实施这些方法以产生商业上显着水平的rAAV颗粒，而不产生显着水平的感染性辅助病毒或其他污染副产物。

公开(公告)号：US07125705B2

公开(公告)日：2006-10-24

申请号：US09839583

申请日：2001-04-20

申请人： Peter Colosi

发明人： Peter Colosi

IPC分类号： C12N7/00 ,  C12N15/00 ,  C12N5/10 ,  C07H21/04

CPC分类号： C12N7/00 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10344 ,  C12N2710/10352 ,  C12N2710/16222 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2750/14152 ,  C12N2800/108 ,  C12N2830/002 ,  C12N2830/75 ,  C12N2840/20 ,  C12N2840/203

摘要： Accessory functions capable of supporting efficient recombinant AAV (rAAV) virion production in a suitable host cell are provided. The accessory functions are in the form of one or more vectors that are capable of being transferred between cells. Methods of producing rAAV virions are also provided. The methods can be practiced to produce commercially significant levels of rAAV particles without also generating significant levels of infectious helper virus or other contaminating by-products.

公开(公告)号：US20050164392A1

公开(公告)日：2005-07-28

申请号：US11043312

申请日：2005-01-26

申请人： Peter Colosi

发明人： Peter Colosi

IPC分类号： C12N15/09 ,  A61K48/00 ,  C07K14/075 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/08 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01 ,  C12N7/04 ,  C12N15/86 ,  C12N15/861 ,  C12N15/864 ,  C12P21/02 ,  C07H21/04

CPC分类号： C12N7/00 ,  A61K48/00 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2710/10362 ,  C12N2750/14143 ,  C12N2750/14151 ,  C12N2830/008

摘要： Accessory functions capable of supporting efficient recombinant AAV (rAAV) virion production in a suitable host cell are provided. The accessory functions are in the form of one or more vectors that are capable of being transferred between cells. Methods of producing rAAV virions are also provided. The methods can be practiced to produce commercially significant levels of rAAV particles without also generating significant levels of infectious helper virus or other contaminating by-products.

公开(公告)号：US07851195B2

公开(公告)日：2010-12-14

申请号：US12214588

申请日：2008-06-20

申请人： Peter Colosi

发明人： Peter Colosi

IPC分类号： C12N7/02

CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2750/14162 ,  C12N2800/30 ,  C12N2840/20

摘要： The present invention provides methods and compositions for producing high titer, wild-type-free preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include novel nucleic acids encoding AAV helper functions and AAV helper function vectors. The present invention also includes host cells transfected by the claimed nucleic acids, methods of using the claimed vectors, and rAAV virions produced by such methods.

摘要翻译： 本发明提供了用于产生高滴度，无重组AAV（“rAAV”）病毒颗粒的无野生型制剂的方法和组合物。 本发明的组合物包括编码AAV辅助功能和AAV辅助功能载体的新型核酸。 本发明还包括由所要求保护的核酸转染的宿主细胞，使用所要求保护的载体的方法，以及通过这些方法产生的rAAV病毒体。

公开(公告)号：US20090017542A1

公开(公告)日：2009-01-15

申请号：US12214588

申请日：2008-06-20

申请人： Peter Colosi

发明人： Peter Colosi

IPC分类号： C12N15/87 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06 ,  C12N7/00

CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2750/14162 ,  C12N2800/30 ,  C12N2840/20

摘要： The present invention provides methods and compositions for producing high titer, wild-type-free preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include novel nucleic acids encoding AAV helper functions and AAV helper function vectors. The present invention also includes host cells transfected by the claimed nucleic acids, methods of using the claimed vectors, and rAAV virions produced by such methods.

公开(公告)号：US20080008690A1

公开(公告)日：2008-01-10

申请号：US11825798

申请日：2007-07-09

申请人： Alejandra Arbetman ,  Peter Colosi ,  Michael Lochrie ,  Richard Surosky

发明人： Alejandra Arbetman ,  Peter Colosi ,  Michael Lochrie ,  Richard Surosky

IPC分类号： A61K48/00 ,  C07H21/04 ,  C07K14/075 ,  C12N15/861

CPC分类号： A61K48/0066 ,  A61K38/4846 ,  A61K48/00 ,  A61K48/0058 ,  A61K48/0075 ,  C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2750/14121 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14162 ,  C12N2830/008 ,  C12N2830/85 ,  C12Y304/21022

摘要： Methods of making and using recombinant AAV virions with decreased immunoreactivity are described. The recombinant AAV virions include mutated capsid proteins or are derived from non-primate mammalian AAV serotypes and isolates that display decreased immunoreactivity relative to AAV-2.

摘要翻译： 描述了制备和使用具有降低的免疫反应性的重组AAV病毒体的方法。 重组AAV病毒粒子包括突变的衣壳蛋白或衍生自非灵长类哺乳动物AAV血清型和相对于AAV-2显示降低的免疫反应性的分离物。