-
1.发明申请公开(公告)号：US20080176273A1
公开(公告)日：2008-07-24
申请号：US11766223
申请日：2007-06-21
Applicant: Gideon Eden , Ruth Eden
Inventor： Gideon Eden , Ruth Eden
Abstract: A new device and method for detecting the presence of living microorganisms in test samples are described. The device comprises a container with at least one section transparent to light, a growth zone located in said container containing a mixture of growth media capable of supporting growth of the microorganisms, and at least one indicator substrate that changes its optical properties due to growth of the microorganisms. A detection zone is located in the container adjacent to the transparent section, and a barrier layer comprising porous solid material separates the two zones, allowing diffusion of molecules and ions of metabolic by-products of the organisms, while preventing microorganisms and particulate matter of the test sample from penetrating into the detection zone.
Abstract translation: 描述了用于检测测试样品中活的微生物的存在的新的装置和方法。 该装置包括具有至少一个对光透明的部分的容器，位于所述容器中的生长区，其包含能够支持微生物生长的生长培养基的混合物，以及至少一个指示剂基质，其由于生长而改变其光学性质 微生物。 检测区域位于与透明部分相邻的容器中，并且包含多孔固体材料的阻挡层分离两个区域，从而允许生物体的代谢副产物的分子和离子的扩散，同时防止微生物和微生物的微生物和颗粒物质 测试样品渗入检测区。
-
公开(公告)号：US5672484A
公开(公告)日：1997-09-30
申请号：US576050
申请日：1995-12-21
Applicant: Ruth Eden , Gideon Eden , Ray McMillian
Inventor： Ruth Eden , Gideon Eden , Ray McMillian
Abstract: A container (10) adapted for use in the detection of aerobic microorganisms in a sample includes a non-toxic insert (22) disposed within the container (10) for supporting microorganisms adhered thereto and for increasing microbial exposure to oxygenated growth media to enhance microbial metabolism. A method for making the container (10) includes the steps of inserting a non-toxic insert (22) into the container (10) and adding growth media (14). Also, a method of detecting aerobic microbiological growth in a sealed sample container (10) having a headspace (16) and which contains a sample which may contain an unknown microorganism includes the steps of providing a sealed sample container (10) having a headspace (16) and non-toxic insert (22) saturated with microbiological growth media (24), inoculating the insert (22) within the sealed sample container (10), and monitoring metabolism in the container (10) as an indicator of the presence of microorganisms to detect microorganisms in the sample.
Abstract translation: 适用于检测样品中需氧微生物的容器（10）包括：设置在容器（10）内的无毒插入物（22），用于支持附着于其上的微生物并增加微生物暴露于含氧生长培养基以增强微生物 代谢。 制造容器（10）的方法包括将无毒插入物（22）插入容器（10）并添加生长培养基（14）的步骤。 此外，在具有顶部空间（16）并且含有可能含有未知微生物的样品的密封样品容器（10）中检测需氧微生物生长的方法包括以下步骤：提供具有顶部空间的密封样品容器（10） 16）和用微生物生长培养基（24）饱和的无毒插入物（22），将插入物（22）接种在密封的样品容器（10）内，并监测容器（10）中的代谢作为 微生物检测样品中的微生物。
-
3.发明授权公开(公告)号：US08557539B2
公开(公告)日：2013-10-15
申请号：US11766223
申请日：2007-06-21
Applicant: Gideon Eden , Ruth Eden
Inventor： Gideon Eden , Ruth Eden
Abstract: A new device and method for detecting the presence of living microorganisms in test samples are described. The device comprises a container with at least one section transparent to light, a growth zone located in said container containing a mixture of growth media capable of supporting growth of the microorganisms, and at least one indicator substrate that changes its optical properties due to growth of the microorganisms. A detection zone is located in the container adjacent to the transparent section, and a barrier layer comprising porous solid material separates the two zones, allowing diffusion of molecules and ions of metabolic by-products of the organisms, while preventing microorganisms and particulate matter of the test sample from penetrating into the detection zone.
Abstract translation: 描述了用于检测测试样品中活的微生物的存在的新的装置和方法。 该装置包括具有至少一个对光透明的部分的容器，位于所述容器中的生长区，其包含能够支持微生物生长的生长培养基的混合物，以及至少一个指示剂基质，其由于生长而改变其光学性质 微生物。 检测区域位于与透明部分相邻的容器中，并且包含多孔固体材料的阻挡层分离两个区域，从而允许生物体的代谢副产物的分子和离子的扩散，同时防止微生物和微生物的微生物和颗粒物质 测试样品渗入检测区。
-
公开(公告)号：US20100273209A1
公开(公告)日：2010-10-28
申请号：US12740078
申请日：2008-01-17
Applicant: Gideon Eden , Ruth Eden
Inventor： Gideon Eden , Ruth Eden
CPC classification number: G01N21/78 , B01L3/5082 , B01L2300/0654 , B01L2300/0851 , C12M41/36 , C12Q1/06 , G01N31/22
Abstract: A new device and method for detecting the presence of living microorganisms in test samples are described. The device includes a container having at least one section transparent to light with an incubation zone defined in the container, the incubation zone containing growth media in which the sample is cultured. A detection zone containing a matrix composed of a polymeric material which is substantially transparent to light, and at least one indicator reagent sensitive to carbon dioxide gas generated by the microorganisms in the incubation zone is located in the transparent section of the matrix. The matrix is configured to facilitate penetration of external light aimed at the transparent section of the container and interaction of the external light with the indicator reagent to yield interactive light that escapes through the transparent section of the container, said interactive light is being indicative of the presence and/or concentration of the microorganisms.
Abstract translation: 描述了用于检测测试样品中活的微生物的存在的新的装置和方法。 该装置包括容器，该容器具有至少一个对容器透明的部分和孵育区域的透明部分，孵育区域包含其中培养样品的生长培养基。 含有由对光基本上透明的聚合物材料构成的基质的检测区域以及由温育区域中的微生物产生的对二氧化碳气体敏感的至少一种指示剂试剂位于基质的透明部分。 该矩阵被配置成便于穿透容器的透明部分的外部光和外部光与指示剂试剂的相互作用，以产生通过容器的透明部分逸出的交互光，所述交互光指示 微生物的存在和/或浓度。
-
公开(公告)号：US5366873A
公开(公告)日：1994-11-22
申请号：US892581
申请日：1992-06-03
Applicant: Gideon Eden , Ruth Eden
Inventor： Gideon Eden , Ruth Eden
CPC classification number: C12Q1/04 , C12M41/36 , C12M41/46 , Y10S435/808
Abstract: A new device and method to detect the presence of live microorganisms are described. The device employs a liquid phase which supports microbial growth by utilizing appropriate media. A secondary phase which consists of semi-liquid material forms a barrier layer through which only small particles can diffuse and which prevent the presence of any large particles. Noise-free optical measurements are carried out at the barrier layer which are indicative of metabolic changes associated with microbial growth.
Abstract translation: 描述了检测活微生物存在的新装置和方法。 该装置采用通过利用适当介质来支持微生物生长的液相。 由半液体材料组成的次相形成阻挡层，只有小颗粒才能扩散并阻止任何大颗粒的存在。 在阻挡层进行无噪声的光学测量，其指示与微生物生长相关的代谢变化。
-
Patent Agency Ranking
公开(公告)号：US09012209B2
公开(公告)日：2015-04-21
申请号：US12740078
申请日：2008-01-17
Applicant: Gideon Eden , Ruth Eden
Inventor： Gideon Eden , Ruth Eden
CPC classification number: G01N21/78 , B01L3/5082 , B01L2300/0654 , B01L2300/0851 , C12M41/36 , C12Q1/06 , G01N31/22
Abstract: A new device and method for detecting the presence of living microorganisms in test samples are described. The device includes a container having at least one section transparent to light with an incubation zone defined in the container, the incubation zone containing growth media in which the sample is cultured. A detection zone containing a matrix composed of a polymeric material which is substantially transparent to light, and at least one indicator reagent sensitive to carbon dioxide gas generated by the microorganisms in the incubation zone is located in the transparent section of the matrix. The matrix is configured to facilitate penetration of external light aimed at the transparent section of the container and interaction of the external light with the indicator reagent to yield interactive light that escapes through the transparent section of the container, said interactive light is being indicative of the presence and/or concentration of the microorganisms.
Abstract translation: 描述了用于检测测试样品中活的微生物的存在的新的装置和方法。 该装置包括容器，该容器具有至少一个对容器透明的部分和孵育区域的透明部分，孵育区域包含其中培养样品的生长培养基。 含有由对光基本上透明的聚合物材料构成的基质的检测区域以及由温育区域中的微生物产生的对二氧化碳气体敏感的至少一种指示剂试剂位于基质的透明部分。 该矩阵被配置成便于穿透容器的透明部分的外部光和外部光与指示剂试剂的相互作用，以产生通过容器的透明部分逸出的交互光，所述交互光指示 微生物的存在和/或浓度。
-
7.发明授权Device and method for the detection and enumeration of multiple groups of microorganisms 有权用于检测和计数多组微生物的装置和方法公开(公告)号：US07745169B2
公开(公告)日：2010-06-29
申请号：US11766208
申请日：2007-06-21
Applicant: Gideon Eden , Ruth Eden
Inventor： Gideon Eden , Ruth Eden
Abstract: A device and method simultaneously detects and enumerates two groups of microorganisms in a test sample, utilizing a single test container. In the container liquid growth media, a chromogenic substrate and a fluorogenic substrate are mixed with the test sample. The test container is incubated to allow bacterial growth and metabolism. Spectral changes of the substrates are dynamically detected using two external light sources aimed at a transparent section of the test container, and a single external photo detector. One light source operates in the visible band and the second in the long ultraviolet band. The two dynamic time patterns generated by the two substrates are analyzed in real time to determine the presence or absence of each microorganisms group and to enumerate their original concentrations in the test sample.
Abstract translation: 一种装置和方法同时利用单个测试容器检测和列举测试样品中的两组微生物。 在容器液体生长培养基中，将显色底物和荧光底物与测试样品混合。 将测试容器温育以允许细菌生长和代谢。 使用瞄准测试容器的透明部分的两个外部光源和单个外部光电检测器来动态地检测衬底的光谱变化。 一个光源在可见光带中工作，第二个在长紫外线带中工作。 实时分析由两个底物产生的两个动态时间图，以确定每个微生物组的存在或不存在，并列举其在测试样品中的原始浓度。
-
8.发明申请Device and Method for the Detection and Enumeration of Multiple Groups of Microorganisms 有权用于检测和计数多组微生物的装置和方法公开(公告)号：US20080113404A1
公开(公告)日：2008-05-15
申请号：US11766208
申请日：2007-06-21
Applicant: Gideon Eden , Ruth Eden
Inventor： Gideon Eden , Ruth Eden
Abstract: A device and method simultaneously detects and enumerates two groups of microorganisms in a test sample, utilizing a single test container. In the container liquid growth media, a chromogenic substrate and a fluorogenic substrate are mixed with the test sample. The test container is incubated to allow bacterial growth and metabolism. Spectral changes of the substrates are dynamically detected using two external light sources aimed at a transparent section of the test container, and a single external photo detector. One light source operates in the visible band and the second in the long ultraviolet band. The two dynamic time patterns generated by the two substrates are analyzed in real time to determine the presence or absence of each microorganisms group and to enumerate their original concentrations in the test sample.
Abstract translation: 一种装置和方法同时利用单个测试容器检测和列举测试样品中的两组微生物。 在容器液体生长培养基中，将显色底物和荧光底物与测试样品混合。 将测试容器温育以允许细菌生长和代谢。 使用瞄准测试容器的透明部分的两个外部光源和单个外部光电检测器来动态地检测衬底的光谱变化。 一个光源在可见光带中工作，第二个在长紫外线带中工作。 实时分析由两个底物产生的两个动态时间图，以确定每个微生物组的存在或不存在，并列举其在测试样品中的原始浓度。
-
公开(公告)号：US20200213629A1
公开(公告)日：2020-07-02
申请号：US16234560
申请日：2018-12-28
Applicant: Gideon Eden
Inventor： Gideon Eden
IPC: H04N21/2187 , H04N21/233 , H04N21/234 , H04N21/61 , H04N21/242
Abstract: The disclosure includes a method of generating and presenting digital streaming data to a viewer. The digital streaming data comprises streaming video data depicted from a live event and streaming audio data generated by a commentator, the streaming audio data related to the live event. In many embodiments, the method includes presenting the streaming video data to the commentator and the viewer and generating streaming audio data by the commentator related to the live event. Methods may also include transferring the streaming audio data via an Internet to the viewer and providing the viewer with synchronization means to synchronize the streaming video data with the streaming audio data.
-
公开(公告)号：US20170089935A1
公开(公告)日：2017-03-30
申请号：US15312236
申请日：2014-05-22
Applicant: Gideon EDEN , Neogen Corporation
Inventor： Gideon Eden
CPC classification number: G01N35/00693 , C12Q1/025 , C12Q1/06 , C12Q1/18 , G01N2035/00702
Abstract: A preservative efficacy test for determining efficiency of at least one preservative material that is present in a consumable product to reduce or eliminate living microorganisms that includes challenging the preservative material with at least one microorganism derived from a pure culture by inoculating a sample of the product with a quantity of at least one challenge microorganism and allowing the microorganisms to grow for an predetermined interval after which the preservative is neutralized and a portion of the resulting sample is cultured in a testing system that includes at least one testing container, wherein the testing container contains liquid growth media and sensor means capable of monitoring microorganism growth by sensing the metabolic by-products generated by growth of microorganisms present in the liquid growth media and producing an output signal that can be measured and analyzed.
-
-
-
-
-
-
-
-
-
Search Results
53
records
(took 0.022 seconds)
