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    Prepro insulin-like growth factors I and II
    1.
    发明授权
    Prepro insulin-like growth factors I and II 失效
    Prepro胰岛素样生长因子I和II

    公开(公告)号：US5405942A

    公开(公告)日：1995-04-11

    申请号：US65673

    申请日：1987-06-16

    申请人： Graeme I. Bell ,  Leslie B. Rall ,  James P. Merryweather

    发明人： Graeme I. Bell ,  Leslie B. Rall ,  James P. Merryweather

    IPC分类号： C12N15/00 ,  A61K38/00 ,  C07H21/04 ,  C07K14/65 ,  C12N15/09 ,  C12N15/17 ,  C12P21/00 ,  C12Q1/68 ,  C12N15/12

    CPC分类号： C07K14/65

    摘要： Polynucleotide sequences which encode for human prepro insulin-like growth factors are provided. Such sequences are obtained from the human genome, typically by screening a cDNA library obtained from human liver cells. The polynucleotide sequences may be used for cloning and expression of insulin-like growth factors in suitable hosts, as well as for the production of DNA and RNA which may be used as hybridization probes.E. coli strains HB101(phigf1) and HB101(phigf2) were deposited at the ATCC on Jun. 8, 1984, and granted accession nos. 39729 and 39730, respectively.

    摘要翻译： 提供编码人前胰岛素样生长因子的多核苷酸序列。 通常通过筛选从人肝细胞获得的cDNA文库，从人类基因组获得这样的序列。 多核苷酸序列可用于在合适的宿主中克隆和表达胰岛素样生长因子，以及用于产生可用作杂交探针的DNA和RNA。 大肠杆菌菌株HB101（phigf1）和HB101（phigf2）于1984年6月8日保藏在ATCC，并授予登录号。 39729和39730。

    Expression using fused genes providing for protein product
    3.
    发明授权
    Expression using fused genes providing for protein product 失效
    使用提供蛋白质产物的融合基因进行表达

    公开(公告)号：US4751180A

    公开(公告)日：1988-06-14

    申请号：US845737

    申请日：1986-03-28

    申请人： Lawrence S. Cousens ,  Patricia A. Tekamp-Olson ,  Jeffrey R. Shuster ,  James P. Merryweather

    发明人： Lawrence S. Cousens ,  Patricia A. Tekamp-Olson ,  Jeffrey R. Shuster ,  James P. Merryweather

    IPC分类号： C07K14/16 ,  C07K14/62 ,  C07K14/65 ,  C12N9/02 ,  C12N15/62 ,  C12N15/81 ,  C12P21/00 ,  C12N5/00 ,  C12N15/00 ,  C12P21/02

    CPC分类号： C07K14/005 ,  C07K14/62 ,  C07K14/65 ,  C12N15/62 ,  C12N15/81 ,  C12N9/0089 ,  C12Y115/01001 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/50 ,  C07K2319/75 ,  C12N2740/16222 ,  Y10S930/31

    摘要： Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield.The S. cerevisiae strain 2150-2-3 (pYASI1) was deposited at the A.T.C.C. on Feb. 27, 1985 and given accession No. 20745.The S. cerevisiae strain AB110 (pYLUIGF2-14) was deposited at the A.T.C.C. on Mar. 19, 1986 and given accession No. 20796.

    摘要翻译： 提供了新的方法和组合物用于提高真菌中异源蛋白质的产量。 所述方法和组合物涉及使用融合序列，所述融合序列涉及编码在相对大量产生的异源产物的序列，作为在开放阅读框中与第二序列融合的宿主中的稳定多肽，其中先前的序列编码不同的异源多肽，其中两个 多肽通过可选择性切割的连接键连接。 特别地，编码超氧化物歧化酶的序列在酵母表达载体的超氧化物歧化酶的任一末端与活性启动子的转录控制和导入酵母宿主和宿主生长的载体连接至另一种目的多肽。 以这种方式获得高产量的融合产物，其中可以选择性地切割融合产物以便以高产率产生超氧化物歧化酶和另一种多肽。 酿酒酵母菌株2150-2-3（pYASI1）保藏在A.T.C.C. 1985年2月27日，加入第20745号。酿酒酵母菌株AB110（pYLUIGF2-14）保藏在A.T.C.C. 1986年3月19日，加拿大号20796。

    Superoxide dismutase fusion polypeptides for expression of mammalian proteins
    4.
    发明授权
    Superoxide dismutase fusion polypeptides for expression of mammalian proteins 失效
    用于表达哺乳动物蛋白质的超氧化物歧化酶融合多肽

    公开(公告)号：US5342921A

    公开(公告)日：1994-08-30

    申请号：US680046

    申请日：1991-03-29

    申请人： Lawrence S. Cousens ,  Patricia A. Tekamp-Olson ,  Jeffrey R. Shuster ,  James P. Merryweather

    发明人： Lawrence S. Cousens ,  Patricia A. Tekamp-Olson ,  Jeffrey R. Shuster ,  James P. Merryweather

    IPC分类号： C07K14/16 ,  C07K14/62 ,  C07K14/65 ,  C12N9/02 ,  C12N15/81 ,  C07K3/00 ,  C07K13/00

    CPC分类号： C07K14/005 ,  C07K14/62 ,  C07K14/65 ,  C12N15/81 ,  C12N9/0089 ,  C12Y115/01001 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/50 ,  C07K2319/75 ,  C12N2740/16222 ,  Y10S435/814

    摘要： Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield.The S. cerevisiae strain 2150-2-3 (pYASI1) was deposited at the A.T.C.C. on Feb. 27, 1985 and given accession no. 20745.The S. cerevisiae strain AB110 (pYLUIGF2-14) was deposited at the A.T.C.C. on Mar. 19, 1986 and given accession no. 20796.

    摘要翻译： 提供了新的方法和组合物用于提高真菌中异源蛋白质的产量。 所述方法和组合物涉及使用融合序列，所述融合序列涉及编码在相对大量产生的异源产物的序列，作为在开放阅读框中与第二序列融合的宿主中的稳定多肽，其中先前的序列编码不同的异源多肽，其中两个 多肽通过可选择性切割的连接键连接。 特别地，编码超氧化物歧化酶的序列在酵母表达载体的超氧化物歧化酶的任一末端与活性启动子的转录控制和导入酵母宿主和宿主生长的载体连接至另一种目的多肽。 以这种方式获得高产量的融合产物，其中可以选择性地切割融合产物以便以高产率产生超氧化物歧化酶和另一种多肽。 酿酒酵母菌株2150-2-3（pYASI1）保藏在A.T.C.C. 1985年2月27日， 酿酒酵母菌株AB110（pYLUIGF2-14）保藏在A.T.C.C. 1986年3月19日，加拿大号。 20796。