Methods and compositions for modulating flowering and maturity in plants
    9.
    发明授权
    Methods and compositions for modulating flowering and maturity in plants 失效
    调节植物开花和成熟的方法和组合物

    公开(公告)号:US07479584B2

    公开(公告)日:2009-01-20

    申请号:US11190339

    申请日:2005-07-27

    CPC分类号: C12N15/827 C07K14/415

    摘要: The present invention provides compositions and methods for modulating flowering time in plants. Maize RAP2.7 nucleotide sequences are disclosed which upon overexpression cause later flowering and when inhibited cause earlier flowering. Also disclosed is a DNA sequence which acts as a regulator/enhancer of RAP2.7, termed VGT1. This sequence does not code for any known protein, but acts as either a RNAi element or a regulatory DNA or RNA element that either directly regulates expression of flowering genes such as Rap2.7 or specifically targets expression of other genes which control flowering genes such as Rap2.7. This element this can be used as a sequence—based marker to identify inbred and hybrids which have altered maturity. Methods for expressing these nucleotide sequences in a plant for modifying maturity and flowering in plants are provided as well as expression constructs, vectors, transformed cells and plants.

    摘要翻译: 本发明提供调节植物开花时间的组合物和方法。 公开了玉米RAP2.7核苷酸序列,其在过表达导致后期开花并且当被抑制时引起早期开花。 还公开了作为RAP2.7调节/增强子的DNA序列,称为VGT1。 该序列不编码任何已知的蛋白质,而是作为直接调节开花基因如Rap2.7的表达的RNAi元件或调节DNA或RNA元件,或者特异性靶向控制开花基因的其他基因的表达,例如 Rap2.7。 这个元素可以用作基于序列的标记来鉴定已经改变成熟度的近交系和杂交种。 提供用于在植物中表达这些核苷酸序列以修饰植物中的成熟和开花的方法以及表达构建体,载体,转化的细胞和植物。

    Br2 and Dw3 promoters and methods of use
    10.
    发明申请
    Br2 and Dw3 promoters and methods of use 有权
    Br2和Dw3启动子和使用方法

    公开(公告)号:US20050204432A1

    公开(公告)日:2005-09-15

    申请号:US10931077

    申请日:2004-08-31

    IPC分类号: A01H5/00 C12N15/82

    摘要: The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, the Dwarf3 (Dw3) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.

    摘要翻译: 本发明涉及基因表达的调节,特别涉及用于调节植物中基因表达的方法和组合物。 提供了包含与Brachytic2(Br2)启动子,Dw3(Dw3)启动子或其功能片段或变体可操作连接的目的多核苷酸分子的表达盒。 另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞,植物组织,植物和种子的方法。 所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。