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    Reaction cassette for preforming sequential analytical assays by noncentrifugal and noncapillary manipulations
    3.
    发明授权
    Reaction cassette for preforming sequential analytical assays by noncentrifugal and noncapillary manipulations 失效
    用于通过非离心和非毛细管理进行顺序分析测定的反应盒

    公开(公告)号：US5162237A

    公开(公告)日：1992-11-10

    申请号：US774362

    申请日：1991-10-08

    申请人： Lowry J. Messenger ,  Christine D. Nelson ,  Kin-Fai Yip ,  Frank W. Wogoman

    发明人： Lowry J. Messenger ,  Christine D. Nelson ,  Kin-Fai Yip ,  Frank W. Wogoman

    IPC分类号： B01L3/00 ,  G01N21/07 ,  G01N33/53 ,  G01N33/543 ,  G01N33/72

    CPC分类号： B01L3/502 ,  G01N21/07 ,  G01N33/5302 ,  G01N33/5304 ,  G01N33/54366 ,  G01N33/723 ,  G01N33/726 ,  Y10S436/808

    摘要： Analytical reaction cassette and method for performing sequential analytical assay procedures to determine the amount of an analyte in a liquid test mixture. The reaction cassette can be in the form of a substantially square container having a substantially horizontal axis of rotation and incorporated with one or more analytical reagents such that they are contacted with a liquid test mixture in a desired ordered sequence to perform a particular assay procedure. Corners provided by the substantially square configuration of the reaction cassette disrupt the flow of liquids disposed in the reaction cassette upon contact therewith to thereby agitate and mix such liquids. A liquid disposed in the reaction cassette is capable of being manipulated and mixed therein by rotating the reaction cassette about the horizontal axis at sufficiently low velocities wherein the transport of such liquid is noncentrifugal and due substantially only to gravitational force. The reaction cassette is particularly useful for performing immunoturbidimetric assays, e.g., for the determination of glycated hemoglobin.

    摘要翻译： 分析反应盒和用于执行顺序分析测定程序以确定液体测试混合物中分析物的量的方法。 反应盒可以是具有基本上水平的旋转轴线的基本上为方形的容器的形式，并结合有一种或多种分析试剂，使得它们以所需的有序序列与液体测试混合物接触以进行特定的测定程序。 通过反应盒的大致正方形配置提供的角部在与反应盒接触时破坏设置在反应盒中的液体的流动，从而搅拌并混合这些液体。 设置在反应盒中的液体能够通过以足够低的速度旋转反应盒围绕水平轴线而被操纵和混合，其中这种液体的输送是非离心的，并且基本上仅仅由重力引起。 反应盒特别可用于进行免疫比浊测定，例如用于测定糖化血红蛋白。

    Method for the determination of hemoglobin adducts
    4.
    发明授权
    Method for the determination of hemoglobin adducts 失效
    血红蛋白加合物测定方法

    公开(公告)号：US6043043A

    公开(公告)日：2000-03-28

    申请号：US77546

    申请日：1993-06-18

    申请人： Kin-Fai Yip

    发明人： Kin-Fai Yip

    IPC分类号： G01N33/53 ,  G01N33/72 ,  G01N15/06 ,  G01N31/00

    CPC分类号： G01N33/723 ,  Y10T436/105831

    摘要： Disclosed is an improvement to the method of determining the concentration of a hemoglobin adduct in a blood sample by the steps of assaying the blood sample for the total amount of hemoglobin, assaying the blood sample for the hemoglobin adduct, and dividing the hemoglobin adduct concentration by the total hemoglobin concentration. The improvement involves normalizing the measurement of the hemoglobin adduct to the total amount of hemoglobin in the blood sample.

    摘要翻译： 公开了通过以下步骤确定血液样品中血红蛋白加合物的浓度的方法的改进：通过测定血液样品中血红蛋白总量，测定血液样品中血红蛋白加合物，并将血红蛋白加合物浓度除以 总血红蛋白浓度。 该改进涉及将血红蛋白加合物的测量标准化为血液样品中血红蛋白的总量。

    Method and apparatus for correcting ambient temperature effect in biosensors
    6.
    发明授权
    Method and apparatus for correcting ambient temperature effect in biosensors 失效
    用于校正生物传感器环境温度影响的方法和装置

    公开(公告)号：US06391645B1

    公开(公告)日：2002-05-21

    申请号：US08854440

    申请日：1997-05-12

    申请人： Dijia Huang ,  Brenda L. Tudor ,  Kin-Fai Yip

    发明人： Dijia Huang ,  Brenda L. Tudor ,  Kin-Fai Yip

    IPC分类号： G01N2712

    CPC分类号： C12Q1/006 ,  G01N27/3274 ,  Y10T436/144444

    摘要： A method and apparatus are provided for correcting ambient temperature effect in biosensors. An ambient temperature value is measured. A sample is applied to the biosensors, then a current generated in the test sample is measured. An observed analyte concentration value is calculated from the current through a standard response curve. The observed analyte concentration is then modified utilizing the measured ambient temperature value to thereby increase the accuracy of the analyte determination. The analyte concentration value can be calculated by solving the following equation: G 2 = G 1 - ( T 2 2 - 24 2 ) * I2 - ( T 2 - 24 ) * I1 ( T 2 2 - 24 2 ) * S2 + ( T 2 - 24 ) * S1 + 1 where G1 is said observed analyte concentration value, T2 is said measured ambient temperature value and I1, I2, S1, and S2 are predetermined parameters.

    摘要翻译： 提供了一种用于校正生物传感器中的环境温度效应的方法和装置。 测量环境温度值。 将样品应用于生物传感器，然后测量在测试样品中产生的电流。 从通过标准响应曲线的电流计算观察到的分析物浓度值。 然后使用测量的环境温度值改变观察到的分析物浓度，从而提高分析物测定的准确度。 分析物浓度值可以通过求解以下公式计算：其中G1是所述观察到的分析物浓度值，T2是所述测量的环境温度值，I1，I2，S1和S2是预定参数。

    Dry reagent for creatinine assay
    7.
    发明授权
    Dry reagent for creatinine assay 失效
    用于肌酐测定的干试剂

    公开(公告)号：US5464777A

    公开(公告)日：1995-11-07

    申请号：US312559

    申请日：1994-09-26

    申请人： Kin-Fai Yip

    发明人： Kin-Fai Yip

    IPC分类号： G01N33/493 ,  G01N33/52 ,  G01N33/53 ,  G01N33/531 ,  G01N33/536 ,  G01N33/70 ,  G01N33/546 ,  G01N33/557

    CPC分类号： G01N33/70 ,  Y10S435/973 ,  Y10T436/147777

    摘要： A method for the determination of protein and creatinine in urine. An organic acid, in the form of its lithium salt, is dried onto a suitable substrate in the second reaction zone of a reaction vessel having a first reaction zone for the immunometric determination of the protein and a second reaction zone for the colorimetric determination of creatinine. After completion of the immunometric determination of the protein the dried organic salt is contacted with the urine containing creatinine resulting in solubilization of the organic acid salt as creatinine reactive reagent thereby facilitating the colorimetric determination of the creatinine.

    摘要翻译： 一种测定尿液中蛋白质和肌酐的方法。 以其锂盐形式的有机酸在具有用于免疫测定蛋白质的第一反应区域的反应容器的第二反应区中被干燥到合适的底物上，以及用于比例测定肌酸酐的第二反应区 。 完成蛋白质的免疫测定后，将干燥的有机盐与含有肌酸酐的尿液接触，导致有机酸盐作为肌酸酐反应试剂的溶解，从而便于肌酸酐的比色测定。

    Crosslinked polyacrylamide-sulfhydryl polymer for immobilization of biologically active substances
    9.
    发明授权
    Crosslinked polyacrylamide-sulfhydryl polymer for immobilization of biologically active substances 失效
    用于固定生物活性物质的交联聚丙烯酰胺 - 巯基聚合物

    公开(公告)号：US4898824A

    公开(公告)日：1990-02-06

    申请号：US939904

    申请日：1986-12-09

    申请人： Kin-Fai Yip

    发明人： Kin-Fai Yip

    IPC分类号： C08F20/56 ,  C08F20/00 ,  C08F220/56 ,  C12N11/08 ,  G01N33/545

    CPC分类号： G01N33/545 ,  C08F220/56 ,  C12N11/08

    摘要： A water-insoluble, crosslinked polyacrylamide copolymer is prepared having recurring monomeric residues of acrylamide, bisacrylamide or analogs thereof, and N,N'-bisacrylylcystamine or analogs thereof. The crosslinked copolymer can be formed into discrete particles having crosslinking disulfide groups on their external surface which can be reduced to provide activated particles having exposed chemically active sulfhydryl groups. The activated polyacrylamide particles are free of residual amino and carboxylic acid groups and can be reacted with suitable haptens and other biologically active substances to provide insolubilized forms thereof.

    摘要翻译： 制备具有丙烯酰胺，双丙烯酰胺或其类似物的重复单体残基和N，N'-双丙烯基胱胺或其类似物的水不溶性交联的聚丙烯酰胺共聚物。 交联共聚物可以在其外表面上形成具有交联二硫键的离散颗粒，其可被还原以提供具有暴露的化学活性巯基的活化颗粒。 活化的聚丙烯酰胺颗粒没有残留的氨基和羧酸基团，并且可以与合适的半抗原和其他生物活性物质反应以提供其不溶性形式。