公开(公告)号：US5408041A

公开(公告)日：1995-04-18

申请号：US180572

申请日：1994-01-13

申请人： Gregory R. Mundy ,  Gloria E. Gutierrez ,  Ian R. Garrett ,  Massimo Sabatini ,  Elzbieta Izbicka ,  Wilson Burgess ,  Gregg R. Crumley ,  Clarence C. Morse ,  Timothy R. Arnett

发明人： Gregory R. Mundy ,  Gloria E. Gutierrez ,  Ian R. Garrett ,  Massimo Sabatini ,  Elzbieta Izbicka ,  Wilson Burgess ,  Gregg R. Crumley ,  Clarence C. Morse ,  Timothy R. Arnett

IPC分类号： C12N15/09 ,  A61K35/32 ,  A61K38/00 ,  A61K38/22 ,  A61K39/395 ,  A61P3/00 ,  A61P43/00 ,  C07K1/16 ,  C07K1/20 ,  C07K1/22 ,  C07K14/51 ,  C07K14/65 ,  C07K16/00 ,  C07K16/22 ,  C12P21/02 ,  C07K1/00 ,  C07K3/00 ,  C07K15/00

CPC分类号： C07K14/65 ,  C07K14/51 ,  C07K16/22 ,  A61K38/00

摘要： The present invention relates to the development of bone growth factors as therapeutics for the prevention and treatment of pathological conditions involving bone tissue. The present invention provides biologically active proteinaceous factors comprising polypeptides containing the amino acid sequence M-A-G-L-G-D-E-F-G-D, [SEQ ID NO: 1], (X) F E T L F G A/V E D V I/D A L Q F V C G D, [SEQ ID NO: 2], or A-Y-I-P-I-E-T-L-E-G-I/G-E-L-V-D/Q-T-G/L-Q-F, [SEQ ID NO: 3], or biologically active fragments or sequence analogues thereof. Among the biological properties of the proteinaceous materials of the present invention is the capability to promote the growth and/or differentiation of osteoblastic cells.

摘要翻译： 本发明涉及作为预防和治疗涉及骨组织的病理状况的治疗剂的骨生长因子的发展。 本发明提供包含含有氨基酸序列MAGLGDEFGD，[SEQ ID NO：1]，（X）FETLFGA / VEDVI / DALQFVCGD，[SEQ ID NO：2]或AYIPIETLEGI / GELVD / QTG / LQF的多肽的生物活性蛋白质因子 ，[SEQ ID NO：3]或其生物活性片段或序列类似物。 本发明的蛋白质材料的生物学特性是促进成骨细胞生长和/或分化的能力。

公开(公告)号：US06187548B1

公开(公告)日：2001-02-13

申请号：US08652877

申请日：1996-05-23

申请人： Göran Akerström ,  Claes Juhlin ,  Lars Rask ,  Göran Hjälm ,  Clarence C. Morse ,  Edward M. Murray ,  Gregg R. Crumley

发明人： Göran Akerström ,  Claes Juhlin ,  Lars Rask ,  Göran Hjälm ,  Clarence C. Morse ,  Edward M. Murray ,  Gregg R. Crumley

IPC分类号： G01N3353

CPC分类号： C07K14/4728 ,  A61K38/00 ,  C07K14/705

摘要： The present invention relates to the isolation of a cDNA clone encoding the calcium sensor in human placenta and subsequent Northern blots confirming the mRNA expression also in human parathyroid and kidney tubule cells. Close sequence similarity is demonstrated with the rat Heymann nephritis antigen, a glycoprotein of the kidney tubule brush border with calcium binding ability. Immunohistochemistry substantiates a tissue distribution of the calcium sensor protein similar to that previously described for the Heymann antigen. It is proposed that the identified calcium sensor protein constitutes a universal sensor for recognition of variation in extracellular calcium, and that it plays a key role for calcium regulation via different organ systems. The calcium sensor protein belongs to the LDL-superfamily of glycoproteins, claimed to function primarily as protein receptors, but with functionally important calcium binding capacity.

摘要翻译： 本发明涉及在人胎盘中分离编码钙传感器的cDNA克隆和随后的Northern印迹，其也证实人甲状旁腺和肾小管细胞中的mRNA表达。 使用大鼠Heymann肾炎抗原（肾小管刷糖蛋白与钙结合能力的糖蛋白）证实了序列相似性。 免疫组织化学证实了钙传感蛋白的组织分布，类似于以前描述的Heymann抗原。 提出鉴定的钙传感器蛋白构成了用于识别细胞外钙的变化的通用传感器，并且其通过不同器官系统对钙调节起关键作用。 钙传感器蛋白属于糖蛋白的LDL超家族，主要作为蛋白质受体起主要作用，但具有功能上重要的钙结合能力。

公开(公告)号：US06720153B1

公开(公告)日：2004-04-13

申请号：US09387176

申请日：1999-08-31

申请人： Richard F. Labaudiniere ,  Kin T. Yu ,  Gregg R. Crumley ,  Clarence C. Morse

发明人： Richard F. Labaudiniere ,  Kin T. Yu ,  Gregg R. Crumley ,  Clarence C. Morse

IPC分类号： G01N3353

CPC分类号： G01N33/502 ,  C12Q1/6897 ,  G01N33/5008 ,  G01N33/6872 ,  G01N33/74

摘要： This invention is directed to a bioassay for determining the functionality of parathyroid hormone compounds. More particularly, this invention is directed to a bioassay wherein the compound to be tested is added to a culture of parathyroid hormone receptor expressing cells bearing a reporter gene under the transcriptional control of multiple c-AMP responsive elements.

摘要翻译： 本发明涉及用于测定甲状旁腺激素化合物的功能的生物测定法。 更具体地说，本发明涉及一种生物测定法，其中将待测化合物加入到具有报告基因的甲状旁腺激素受体表达细胞的培养基中，在多个c-AMP响应元件的转录控制下。

公开(公告)号：US06239270B1

公开(公告)日：2001-05-29

申请号：US08476515

申请日：1995-06-07

申请人： Göran Akerström ,  Claes Juhlin ,  Lars Rask ,  Göran Hjälm ,  Clarence C. Morse ,  Edward M. Murray ,  Gregg R. Crumley

发明人： Göran Akerström ,  Claes Juhlin ,  Lars Rask ,  Göran Hjälm ,  Clarence C. Morse ,  Edward M. Murray ,  Gregg R. Crumley

IPC分类号： A61K317105

CPC分类号： C07K14/705 ,  A61K38/00

摘要： The present invention relates to the isolation of a cDNA clone encoding the calcium sensor in human placenta and subsequent Northern blots confirming the mRNA expression also in human parathyroid and kidney tubule cells. Close sequence similarity is demonstrated with the rat Heymann nephritis antigen, a glycoprotein of the kidney tubule brush border with calcium binding ability. Immunohistochemistry substantiates a tissue distribution of the calcium sensor protein similar to that previously described for the Heymann antigen. It is proposed that the identified calcium sensor protein constitutes a universal sensor for recognition of variation in extracellular calcium, and that it plays a key role for calcium regulation via different organ systems. The calcium sensor protein belongs to the LDL-superfamily of glycoproteins, claimed to function primarily as protein receptors, but with functionally important calcium binding capacity.

摘要翻译： 本发明涉及在人胎盘中分离编码钙传感器的cDNA克隆和随后的Northern印迹，其也证实人甲状旁腺和肾小管细胞中的mRNA表达。 使用大鼠Heymann肾炎抗原（肾小管刷糖蛋白与钙结合能力的糖蛋白）证实了序列相似性。 免疫组织化学证实了钙传感蛋白的组织分布，类似于以前描述的Heymann抗原。 提出鉴定的钙传感器蛋白构成了用于识别细胞外钙的变化的通用传感器，并且其通过不同器官系统对钙调节起关键作用。 钙传感器蛋白属于糖蛋白的LDL超家族，主要作为蛋白质受体起主要作用，但具有功能上重要的钙结合能力。