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公开(公告)号：US07135624B2
公开(公告)日：2006-11-14
申请号：US10221074
申请日：2001-03-09
CPC分类号： C12N15/8243 , A23J3/16 , A23L33/185 , C12N9/90 , C12N15/8209 , C12N15/8242
摘要： This invention relates to an isolated nucleic acid fragment encoding an oxidosqualene cyclase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the isolated polynucleotides of the invention, in sense or antisense orientation, operably linked to a suitable regulatory sequence.
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2.发明授权Compositions with increased phytosterol levels obtained from plants with decreased triterpene saponin levels 失效具有降低的三萜皂苷水平的植物获得的具有增加的植物甾醇水平的组合物公开(公告)号：US07750210B2
公开(公告)日：2010-07-06
申请号：US11593880
申请日：2006-11-07
CPC分类号： C12N15/8243 , C12N9/90
摘要： This invention is in the field of plant molecular biology. More specifically, this invention pertains to compositions having increased levels of at least one phytosterol, said compositions being obtained from plants or plant parts, and methods thereof. The plants may have decreased levels of triterpene saponins. The plants or plant parts may comprise at least one recombinant DNA molecule comprising a promoter operably linked to at least a portion of at least one polynucleotide from at least one oxidosqualene cyclase gene, said recombinant DNA molecule sufficient to increase the production of at least one phytosterol; or any progeny of said plant, wherein said progeny comprise said recombinant DNA molecule. Compositions, oils, as well as food and feed products obtained from or prepared with said compositions are also part of the invention.
摘要翻译： 本发明在植物分子生物学领域。 更具体地，本发明涉及具有增加的至少一种植物甾醇水平的组合物，所述组合物从植物或植物部分获得，及其方法。 植物可能具有降低的三萜皂苷的水平。 植物或植物部分可以包含至少一个重组DNA分子，其包含可操作地连接至少一个至少一部分来自至少一个氧化四烯环化酶基因的多核苷酸的启动子，所述重组DNA分子足以增加至少一种植物甾醇的产生 ; 或所述植物的任何后代，其中所述后代包含所述重组DNA分子。 由所述组合物获得或制备的组合物，油以及食品和饲料产品也是本发明的一部分。
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公开(公告)号：US08877503B2
公开(公告)日：2014-11-04
申请号：US13107212
申请日：2011-05-13
IPC分类号： C12N15/82 , A01H9/00 , C07H21/04 , C07K14/195 , C12N15/63 , C07K14/245
CPC分类号： C07K14/245 , C07K14/195 , C12N15/635 , C12N15/8217 , C12N15/8238 , C12N15/8278
摘要： Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.
摘要翻译： 提供了与使用磺酰脲类阻遏物相关的组合物和方法。 组合物包括特异性结合操作者的多肽，其中特异性结合受磺酰脲化合物调节。 组合物还包括编码多肽的多核苷酸以及构建体，载体，原核和真核细胞，以及真核生物，包括包含多核苷酸的植物和种子，和/或由该方法产生的。 还提供了向细胞或生物体提供磺酰脲反应性阻遏物并调节目标多核苷酸在包括植物或植物细胞的细胞或生物体中的表达的方法。
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公开(公告)号：US20120295354A1
公开(公告)日：2012-11-22
申请号：US13563854
申请日：2012-08-01
IPC分类号： C12N5/10 , C07K14/245 , C07H21/04 , C12N1/21 , C07K14/00
CPC分类号： C07K14/245 , C07K14/195 , C12N15/635 , C12N15/8217 , C12N15/8238 , C12N15/8278
摘要： Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.
摘要翻译： 提供了与使用磺酰脲类阻遏物相关的组合物和方法。 组合物包括特异性结合操作者的多肽，其中特异性结合受磺酰脲化合物调节。 组合物还包括编码多肽的多核苷酸以及构建体，载体，原核和真核细胞，以及真核生物，包括包含多核苷酸的植物和种子，和/或由该方法产生的。 还提供了向细胞或生物体提供磺酰脲应答阻遏物并调节感兴趣的多核苷酸在包括植物或植物细胞的细胞或生物体中的表达的方法。
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公开(公告)号：US08115055B2
公开(公告)日：2012-02-14
申请号：US12335704
申请日：2008-12-16
申请人： Brian McGonigle
发明人： Brian McGonigle
CPC分类号： C12N15/8218 , C12N15/8247 , C12N15/825
摘要： Isolated nucleic acid fragments comprising precursor miRNA, and artificial miRNAs and their use in down-regulating gene expression are described.
摘要翻译： 描述了包含前体miRNA和人造miRNA的分离的核酸片段及其在下调基因表达中的用途。
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6.发明申请公开(公告)号：US20100175148A1
公开(公告)日：2010-07-08
申请号：US12725482
申请日：2010-03-17
IPC分类号： C12P7/64 , C07H21/04 , C12N9/02 , C12N15/82 , C12N5/10 , C12N15/63 , A01H1/00 , A01H5/10 , A01H5/00
CPC分类号： C12N15/8247 , C11B1/025 , C12N9/1029 , C12P7/6427 , C12P7/6463 , C12P7/6472
摘要： Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.
摘要翻译： 分离的核酸片段和包含编码Δ-9延伸酶的这种片段的重组构建物以及在植物中使用这些δ-9延长酶制备长链多不饱和脂肪酸（PUFA）的方法。
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7.发明申请NOVEL DELTA-9 FATTY ACID ELONGASE GENES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS 有权新型DELTA-9脂肪酸快速基因及其在制备聚氨酯脂肪酸中的应用公开(公告)号：US20090320161A1
公开(公告)日：2009-12-24
申请号：US12487768
申请日：2009-06-19
CPC分类号： C12N15/8247 , A23D9/00 , C12N9/1029 , C12P7/6427
摘要： Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding novel delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.
摘要翻译： 分离的核酸片段和包含编码新的Δ-9延伸酶的这种片段的重组构建物以及在植物中使用这些δ-9延长酶制备长链多不饱和脂肪酸（PUFA）的方法。
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公开(公告)号：US20080276333A1
公开(公告)日：2008-11-06
申请号：US11596037
申请日：2005-06-09
申请人： Brian McGonigle , Johan M. Stoop
发明人： Brian McGonigle , Johan M. Stoop
CPC分类号： C12N15/8247 , C12N15/8216 , C12N15/8218 , C12N15/8245
摘要： Recombinant constructs useful for reducing the expression of at least one target nucleic acid fragment of interest and any substantially similar endogenous nucleic acid fragment of interest are disclosed. In particular, a recombinant construct comprising at least one nucleic acid of interest is inserted between two convergent promoters. A plant or plant organ stably transformed with this construct will have a reduction in expression of the target nucleic acid fragments of interest.
摘要翻译： 公开了可用于降低目标的至少一个靶核酸片段和任何基本上相似的感兴趣的内源性核酸片段的表达的重组构建体。 特别地，将包含至少一种目的核酸的重组构建体插入两个收敛启动子之间。 用该构建体稳定转化的植物或植物器官将具有目标靶核酸片段的表达降低。
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公开(公告)号：US20060031961A1
公开(公告)日：2006-02-09
申请号：US11198728
申请日：2005-08-05
申请人： Brian McGonigle , John Everard , David Nekoukar
发明人： Brian McGonigle , John Everard , David Nekoukar
CPC分类号： C12N9/0006 , A61K36/48 , C12Y101/01002
摘要： This invention relates to an isolated polynucleotide encoding an aldehyde reductase enzyme. The invention also relates to the construction of a recombinant construct encoding an aldehyde reductase enzyme wherein expression of the recombinant construct results in production of altered levels of aldehyde reductase enzyme in a transformed host cell. The present invention also concerns soybean products made from seeds obtained from soybean plants transformed with this recombinant construct wherein such seeds having increased aldehyde reductase activity.
摘要翻译： 本发明涉及编码醛还原酶的分离的多核苷酸。 本发明还涉及编码醛还原酶的重组构建体的构建，其中重组构建体的表达导致在转化的宿主细胞中产生醛还原酶水平的变化。 本发明还涉及由用这种重组构建体转化的大豆植物获得的种子制成的大豆产品，其中这种种子具有增加的醛还原酶活性。
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公开(公告)号：US6054638A
公开(公告)日：2000-04-25
申请号：US984550
申请日：1997-12-03
申请人： Brian McGonigle , Daniel P. O'Keefe
发明人： Brian McGonigle , Daniel P. O'Keefe
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , C12N15/00 , A01H4/00
CPC分类号： C07K14/415 , C12N15/8241 , C12N15/8278
摘要： This invention relates to the isolation of nucleic acid fragments from soybean that encode ADP ribosylation factor (ARF) protein. This invention also relates to nucleic acid fragments encoding proteins that are capable of conferring herbicide tolerance to chlorimuron-ethyl, a sulfonylurea herbicide. The invention encompasses genetic screens for crop protection chemicals, transgenic plants, and breeding methods.
摘要翻译： 本发明涉及从编码ADP核糖基化因子（ARF）蛋白的大豆中分离核酸片段。 本发明还涉及编码蛋白质的核酸片段，其能够赋予除草剂对氯嘧磺隆 - 磺酰脲除草剂的耐受性。 本发明包括作物保护化学品，转基因植物和育种方法的遗传筛选。
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