公开(公告)号：US20220119756A1

公开(公告)日：2022-04-21

申请号：US17565929

申请日：2021-12-30

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Natsumi SAITO ,  Taira Ito ,  Takuji Kataoka

IPC: C12M1/42 ,  G01N21/64 ,  C12M1/34 ,  G01N33/483 ,  G01N33/487

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.

公开(公告)号：US20150276708A1

公开(公告)日：2015-10-01

申请号：US14438055

申请日：2013-10-23

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Takuji Kataoka ,  Taira Ito ,  Natsumi Saito

IPC: G01N33/483 ,  G01N15/14

CPC classification number: G01N33/4836 ,  G01N15/1031 ,  G01N15/1431 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1456 ,  G01N15/1484 ,  G01N21/6452 ,  G01N2015/0065 ,  G01N2015/1006

Abstract: A cell observation system 1, for measuring fluorescence emitted from a cell held by a microplate 20 having a plurality of wells 21, comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, a position controller 30 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21, a moving image acquisition unit 40 for detecting the fluorescence from the sample S within the wells 21, and a data analyzer 50 for setting a part of a region facing the positive electrode 17b on the well 21 as an analysis region and analyzing an optical intensity in the analysis region so as to acquire analysis information concerning the sample S.

Abstract translation: 用于测量由具有多个孔21的微板20保持的细胞发出的荧光的细胞观察系统1包括用于安装微板20的微孔板保持器11，布置成包括阳性的多个电极对17的电刺激器16 和负极17b，17a，用于控制电刺激器16的位置以将电极对17放置在井21内的位置控制器30，用于检测孔内的样品S的荧光的运动图像获取单元40 21，以及数据分析器50，用于将在井21上面对正极17b的区域的一部分设置为分析区域，并分析分析区域中的光强度，以便获取关于样品S的分析信息。

公开(公告)号：US11248202B2

公开(公告)日：2022-02-15

申请号：US16527423

申请日：2019-07-31

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Natsumi Saito ,  Taira Ito ,  Takuji Kataoka

IPC: C12M1/34 ,  C12M1/42 ,  G01N21/64 ,  G01N33/483 ,  G01N33/487

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.

公开(公告)号：US10415010B2

公开(公告)日：2019-09-17

申请号：US15110678

申请日：2014-11-12

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Natsumi Saito ,  Taira Ito ,  Takuji Kataoka

IPC: C12M1/42 ,  G01N21/64 ,  G01N33/487 ,  C12M1/34 ,  G01N33/483

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.

公开(公告)号：US20150276599A1

公开(公告)日：2015-10-01

申请号：US14437964

申请日：2013-10-23

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Takuji Kataoka ,  Taira Ito ,  Natsumi Saito

IPC: G01N21/64 ,  G01N33/483

CPC classification number: G01N21/6428 ,  G01N33/4836 ,  G01N33/48728 ,  G01N33/5005 ,  G01N2201/022

Abstract: A cell observation system 1 is a cell observation system 1 for observing a cell held by a microplate 20 having a plurality of wells 21 arranged therein for holding a sample S including the cell and comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, and a data analyzer 50 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21 of the microplate 20, while a leading end of the negative electrode 17a on the well 21 side extends longer than a leading end of the positive electrode 17b on the well 21 side.

Abstract translation: 细胞观察系统1是细胞观察系统1，用于观察由微孔板20保持的细胞，该微孔板20具有布置在其中的多个孔21，用于保持包括细胞的样品S，并且包括用于安装微板20的微孔板保持器11， 激励器16，其配置有包括正极和负极17b，17a的多个电极对17，以及用于控制电刺激器16的位置以将电极对17放置在微板20的孔21内的数据分析器50， 而在井21侧的负极17a的前端比阱21侧的正极17b的前端延伸。

公开(公告)号：US09778189B2

公开(公告)日：2017-10-03

申请号：US14437964

申请日：2013-10-23

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Takuji Kataoka ,  Taira Ito ,  Natsumi Saito

IPC: G01N15/10 ,  G01N15/14 ,  G01N33/48 ,  G01N21/64 ,  G01N33/487 ,  G01N33/483 ,  G01N33/50

CPC classification number: G01N21/6428 ,  G01N33/4836 ,  G01N33/48728 ,  G01N33/5005 ,  G01N2201/022

Abstract: A cell observation system 1 is a cell observation system 1 for observing a cell held by a microplate 20 having a plurality of wells 21 arranged therein for holding a sample S including the cell and comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, and a data analyzer 50 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21 of the microplate 20, while a leading end of the negative electrode 17a on the well 21 side extends longer than a leading end of the positive electrode 17b on the well 21 side.

公开(公告)号：US09772322B2

公开(公告)日：2017-09-26

申请号：US14438055

申请日：2013-10-23

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Takuji Kataoka ,  Taira Ito ,  Natsumi Saito

IPC: G01N15/00 ,  G01N21/00 ,  G01N33/48 ,  G01N33/483 ,  G01N21/64 ,  G01N15/14 ,  G01N15/10

CPC classification number: G01N33/4836 ,  G01N15/1031 ,  G01N15/1431 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1456 ,  G01N15/1484 ,  G01N21/6452 ,  G01N2015/0065 ,  G01N2015/1006

Abstract: A cell observation system 1, for measuring fluorescence emitted from a cell held by a microplate 20 having a plurality of wells 21, comprises a microplate holder 11 for mounting the microplate 20, an electrical stimulator 16 arranged with a plurality of electrode pairs 17 including positive and negative electrodes 17b, 17a, a position controller 30 for controlling a position of the electrical stimulator 16 so as to place the electrode pairs 17 within the wells 21, a moving image acquisition unit 40 for detecting the fluorescence from the sample S within the wells 21, and a data analyzer 50 for setting a part of a region facing the positive electrode 17b on the well 21 as an analysis region and analyzing an optical intensity in the analysis region so as to acquire analysis information concerning the sample S.