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公开(公告)号:US11358390B2
公开(公告)日:2022-06-14
申请号:US17251856
申请日:2018-09-27
Applicant: Hewlett-Packard Development Company, L.P.
Inventor: Michael Cumbie , Chien-Hua Chen
Abstract: Examples include a fluid ejection device comprising a carrier, at least one fluid ejection die, and conductive traces at least partially embedded in the carrier. The carrier has a first portion and a second portion, where an angle of orientation between the first portion and the second portion is nonparallel. The first portion includes an array of openings formed through a top surface of the carrier. The second portion includes at least one die opening through a bottom surface of the carrier. The fluid ejection die is coupled to the second portion of the carrier. Fluid passages formed in a back surface of the fluid ejection die are exposed through the at least one die opening formed through the bottom surface of the carrier. The conductive traces have an array of contact points at first ends of the conductive traces. The array of contact points align with the array of openings of the first portion of the carrier such that the array of contact points are exposed through the array of openings. The conductive traces connect the fluid ejection die and the array of contact points.
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公开(公告)号:US11215562B2
公开(公告)日:2022-01-04
申请号:US16612786
申请日:2017-08-28
Applicant: Hewlett-Packard Development Company, L.P.
Inventor: Chien-Hua Chen , Michael Cumbie , Viktor Shkolnikov
Abstract: Examples include a fluid device. The fluid device includes a substrate, a sensor coupled on the substrate. A reservoir is formed in the substrate adjacent to the sensor. A deformable cover is disposed to seal the sensor and the reservoir on the substrate.
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公开(公告)号:US20240367171A1
公开(公告)日:2024-11-07
申请号:US18557564
申请日:2021-04-30
Applicant: Hewlett-Packard Development Company, L.P.
Inventor: Michael Cumbie , Viktor Shkolnikov
Abstract: A microfluidic device is described. The device comprises a reaction chamber, wherein the reaction chamber comprises at least one reaction reagent disposed on at least one inner surface of the reaction chamber. A heater is also provided. A thermally dissolvable or degradable or thermally degradable film is applied to the at least one inner surface of the reaction chamber on which the reaction reagent is disposed. Also described is a PCR apparatus and a method of performing PCR.
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公开(公告)号:US20240216916A1
公开(公告)日:2024-07-04
申请号:US18557860
申请日:2021-04-30
Applicant: Hewlett-Packard Development Company, L.P.
Inventor: David Olsen , Michael Cumbie , Viktor Shkolnikov
CPC classification number: B01L3/502723 , C12N15/1013 , C12Q1/686 , B01L2200/16 , B01L2300/041 , B01L2300/0816 , B01L2400/0406 , B01L2400/084
Abstract: A microfluidic device is described. The device comprises a flow channel; and at least one reaction chamber. The at least one reaction chamber comprises a chamber inlet connecting the at least one reaction chamber to the flow channel, a first region adjacent the chamber inlet, a second region spaced from the chamber inlet by the first region, a vent channel and a reaction reagent disposed on at least one inner surface of the second region. Also described is a PCR apparatus and a method of performing PCR.
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公开(公告)号:US20210252859A1
公开(公告)日:2021-08-19
申请号:US17251856
申请日:2018-09-27
Applicant: Hewlett-Packard Development Company, L.P.
Inventor: Michael Cumbie , Chien-Hua Chen
IPC: B41J2/14
Abstract: Examples include a fluid ejection device comprising a carrier, at least one fluid ejection die, and conductive traces at least partially embedded in the carrier. The carrier has a first portion and a second portion, where an angle of orientation between the first portion and the second portion is nonparallel. The first portion includes an array of openings formed through a top surface of the carrier. The second portion includes at least one die opening through a bottom surface of the carrier. The fluid ejection die is coupled to the second portion of the carrier. Fluid passages formed in a back surface of the fluid ejection die are exposed through the at least one die opening formed through the bottom surface of the carrier. The conductive traces have an array of contact points at first ends of the conductive traces. The array of contact points align with the array of openings of the first portion of the carrier such that the array of contact points are exposed through the array of openings. The conductive traces connect the fluid ejection die and the array of contact points.
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公开(公告)号:US20250121375A1
公开(公告)日:2025-04-17
申请号:US18702194
申请日:2021-10-22
Applicant: Hewlett-Packard Development Company, L.P.
Inventor: Michael Cumbie , Viktor Shkolnikov , Brian J. Keefe
Abstract: A PCR system is described. The PCR system comprises a microfluidic cartridge having a thermocycling chamber, wherein a floor of the thermocycling chamber is substantially planar and is provided with a heater; a cooling module configured to engage with and be in thermal contact with a surface of the microfluidic cartridge; and an optical sensor configured to obtain optical signals from the thermocycling chamber. A method of performing PCR is also described.
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公开(公告)号:US20240218434A1
公开(公告)日:2024-07-04
申请号:US18557281
申请日:2021-04-30
Applicant: Hewlett-Packard Development Company, L.P.
Inventor: Michael Cumbie , Brian J Keefe , Kenza Samlali , Viktor Shkolnikov
IPC: C12Q1/6844 , B01L3/00 , B01L7/00
CPC classification number: C12Q1/6844 , B01L3/502715 , B01L3/502792 , B01L7/52 , B01L2200/16 , B01L2300/0645 , B01L2300/0654 , B01L2400/0427
Abstract: A PCR system is described. The PCR system comprises a thermocycling chamber comprising an electrode assembly configured to generate an electric field and cause reversible electrowetting of a PCR mixture within the thermocycling chamber; and an optical sensor configured to obtain optical signals from the thermocycling chamber. Also described is a method of manufacturing a PCR system and a method of performing PCR.
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公开(公告)号:US20240216912A1
公开(公告)日:2024-07-04
申请号:US18557567
申请日:2021-04-30
Applicant: Hewlett-Packard Development Company, L.P.
Inventor: Michael Cumbie , Viktor Shkolnikov , Chien-Hua Chen
IPC: B01L3/00
CPC classification number: B01L3/502715 , B01L3/502707 , B01L2200/025 , B01L2200/12 , B01L2300/0663 , B01L2300/12 , B01L2300/1805
Abstract: A microfluidic device is described. The microfluidic device comprises a reaction chamber and a temperature control module overlaying the reaction chamber. The temperature control module comprises a temperature regulating flow channel disposed between two layers and in thermal contact with the reaction chamber, and wherein at least a portion of each layer comprises an optically transparent material. Also described is a method of manufacturing a microfluidic device and a method of controlling temperature of a reaction in a microfluidic device.
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公开(公告)号:US20250011849A1
公开(公告)日:2025-01-09
申请号:US18702182
申请日:2021-10-22
Applicant: Hewlett-Packard Development Company, L.P.
Inventor: Michael Cumbie , Viktor Shkolnikov
IPC: C12Q1/6844 , B01L3/00 , B01L7/00
Abstract: A digital droplet PCR system is described. The digital droplet PCR system comprises a microfluidic cartridge comprising: a plurality of sample droplet generators, wherein each sample droplet generator is operable to partition a PCR mixture into a plurality of aqueous droplets dispersed in a carrier liquid, and wherein at least one sample droplet generator is operable to partition a PCR mixture that is different to a PCR mixture that is partitioned by at least one other sample droplet generator; a thermocycling chamber comprising an embedded heater, an inlet configured to receive the plurality of aqueous droplets from the plurality of sample droplet generators, and an outlet; and an optical readout zone fluidly connected to the outlet of the thermocycling chamber; and a pressure actuated pump configured to couple to and cause fluid flow through the microfluidic cartridge. A method of performing digital droplet PCR is also described.
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公开(公告)号:US20230374435A1
公开(公告)日:2023-11-23
申请号:US18215105
申请日:2023-06-27
Applicant: Hewlett-Packard Development Company, L.P.
Inventor: Viktor Shkolnikov , Diane Hammerstad , Michael Cumbie
CPC classification number: C12M35/02 , C12M33/04 , C12M41/48 , C12N13/00 , C12N15/87 , C12M3/006 , C12M1/42 , A61N1/10
Abstract: An electroporation system may include a well plate, a dispenser and a dispenser-well positioning system. The well plate may include wells, each of the wells including an interior, a first electrode adjacent the interior and a second electrode adjacent the interior and spaced from the first electrode. The first electrode and the second electrode are to apply an electrostatic field across the well. The dispenser is to dispense a cell having a diameter into each of the wells. The dispenser-well positioning system is to align each well and the dispenser such that the dispenser dispenses the cell into each well at a location spaced from the first electrode and the second electrode by a distance of at least 5 times the diameter of the cell.
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