公开(公告)号：US10030266B2

公开(公告)日：2018-07-24

申请号：US14895923

申请日：2013-06-27

Applicant: HITACHI, LTD.

Inventor： Itaru Yanagi ,  Riichiro Takemura ,  Yoshimitsu Yanagawa ,  Takahide Yokoi ,  Takashi Anazawa

IPC: C12Q1/6874 ,  H01L29/49 ,  H01L29/66 ,  G01N27/414 ,  H01L21/02 ,  H01L21/283 ,  H01L21/306 ,  H01L21/8234

Abstract: In the field of the next generation DNA sequencer, a method for integrating very high sensitive FET sensors having side gates and nanopores as devices used for identifying four kinds of base and for mapping the base sequence of DNA without using reagents, and a semiconductor device having selection transistors and amplifier transistors respectively corresponding to the FET sensors having side gates and nanopores respectively so as to be able to read the variation of a detection current based on the differences among the charges of the four kinds of base without deteriorating the detection sensitivity of the FET sensor, are presented.

公开(公告)号：US20140243214A1

公开(公告)日：2014-08-28

申请号：US14241041

申请日：2013-02-26

Applicant: Hitachi, Ltd.

Inventor： Takanobu Haga ,  Itaru Yanagi ,  Naoshi Itabashi ,  Yoshimitsu Yanagawa ,  Takeshi Ohura ,  Takashi Anazawa

IPC: G01N33/487 ,  G01N27/414

CPC classification number: G01N33/48721 ,  G01N27/4145 ,  Y10T436/143333

Abstract: In an FET configuration having a channel with a small thickness, transistor characteristics vary for different FETs in the same array, and therefore when the same gate voltage is applied, the sensitivities of DNA detection may be insufficient. To this end, the change in the channel current when DNA passes through the nanopore is detected while applying an optimum gate voltage for each nanopore FET to attain a predetermined channel current value to a plurality of nanopore FETs disposed on the same substrate, and four types of bases constituting DNA are distinguished.

Abstract translation: 在具有小厚度的沟道的FET配置中，晶体管特性对于同一阵列中的不同FET而变化，因此当施加相同的栅极电压时，DNA检测的灵敏度可能不足。 为此，检测DNA通过纳米孔时的通道电流的变化，同时对每个纳米孔FET施加最佳的栅极电压，以对设置在同一基板上的多个纳米孔FET获得预定的通道电流值， 区分构成DNA的碱基。

公开(公告)号：US20240009667A1

公开(公告)日：2024-01-11

申请号：US18335246

申请日：2023-06-15

Applicant: HITACHI, LTD.

Inventor： Saaya Shimizu ,  Ryo Imai ,  Yoshimitsu Yanagawa

IPC: B01L3/00 ,  B01L9/00

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0689 ,  B01L2200/143 ,  B01L2400/0406 ,  B01L2400/0481

Abstract: Provided is a liquid feeding method for removing bubbles from a solution introduced into a fluidic device, wherein: the fluidic device includes a flow path of a volume v, a first chamber having a volume V1 and connected to a first side of the flow path, and a second chamber having a volume V2 and connected to a second side, being different from the first side, of the flow path; V1, V2, and v satisfy Formula (1) and Formula (2), α is a solubility of gas in the solution at a temperature of the second chamber, β is a solubility of gas in the solution at a temperature of the flow path, P0 is a pressure of a surrounding environment of the fluidic device, ΔP is a bubble internal pressure rise value, where ΔP=4σ/d, σ is a surface tension of the solution, and d is a diameter of the flow path; and the liquid feeding method includes: disposing the solution at a first position opposite to the flow path with respect to the second chamber; pressurizing and feeding the solution so that the solution is transferred through the second chamber toward the flow path; and pressurizing and introducing the solution from the second chamber into the flow path.

公开(公告)号：US20240052507A1

公开(公告)日：2024-02-15

申请号：US18260879

申请日：2021-11-30

Applicant: HITACHI, LTD.

Inventor： Itaru Yanagi ,  Yoshimitsu Yanagawa

IPC: C25B13/02

CPC classification number: C25B13/02

Abstract: Realized is a formation of a nanopore having a desired size with high yields using the dielectric breakdown phenomenon. The present disclosure proposes, as an aspect, a pore forming method including disposing a membrane between a first electrolyte solution and a second electrolyte solution held in a chamber configuration, bringing a first electrode into contact with the first electrolyte solution and a second electrode into contact with the second electrolyte solution, outputting a first voltage from a voltage source to a circuit configured by disposing the voltage source and a resistor in a wiring connecting the first electrode and the second electrode, measuring a second voltage between the first electrode and the second electrode, detecting a significant change in the second voltage, and stopping output of the first voltage in response to detecting the significant change in the second voltage.

公开(公告)号：US20140154790A1

公开(公告)日：2014-06-05

申请号：US14122206

申请日：2011-05-31

Applicant: HITACHI, LTD.

Inventor： Kazuo Ono ,  Tatsuo Nakagawa ,  Yoshimitsu Yanagawa ,  Takayuki Kawahara ,  Akira Kotabe ,  Riichiro Takemura

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2565/631

Abstract: Provided is a device that, on the basis of a measurement result of a current that has a low value and a wide distribution, identifies the composition of biological molecules passing through a nanoparticle path. This biomolecule information analysis device obtains a current value by applying an electrical field to biomolecules passing through a gap between a first electrode and a second electrode, and identifies the structure of the biomolecules by integrating the current value and making a comparison with a reference value (see FIG. 1).

Abstract translation: 提供了基于具有低值和广泛分布的电流的测量结果来识别通过纳米颗粒路径的生物分子的组成的装置。 该生物分子信息分析装置通过对通过第一电极和第二电极之间的间隙的生物分子施加电场来获得电流值，并且通过对当前值进行积分并与参考值进行比较来识别生物分子的结构（ 参见图1）。