公开(公告)号：US10138456B2

公开(公告)日：2018-11-27

申请号：US15327208

申请日：2015-07-08

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Akihiro Shimase ,  Kazumichi Imai ,  Sadamitsu Aso ,  Eiichiro Takada ,  Masako Kawarai ,  Toshinari Sakurai

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/34 ,  C12M1/02 ,  C12M1/36 ,  C12Q1/06

Abstract: A device which automatically performs a step in which expanded and cultured cells are diluted to a desired cell concentration and re-inoculated using a cell-concentration adjustment device having an inlet for taking in a cell suspension; an outlet for discharging a diluted cell suspension; and a flow path which is provided between the inlet and the outlet and is capable of holding a cell suspension, the flow path being provided with: a liquid delivery pump for causing a cell suspension inside to flow; a cell-concentration measurement instrument for collecting data related to a cell concentration per unit amount of the cell suspension; and a dilution-liquid container for holding a dilution liquid which is supplied to the flow path to dilute the cell suspension. The device further includes a control unit for controlling at least the liquid delivery pump on the basis of the data obtained by the cell-concentration measurement instrument, wherein the control unit determines, on the basis of the data obtained by the cell-concentration measurement instrument, an amount of the dilution liquid required to bring the cell concentration to the desired concentration, and drives the liquid delivery pump so as to take in the required amount of the dilution liquid into the flow path and mix the cell suspension and the dilution liquid.

公开(公告)号：US10208277B2

公开(公告)日：2019-02-19

申请号：US15107548

申请日：2014-12-24

Applicant: Hitachi High-Technologies Corporation

Inventor： Akihiro Shimase ,  Kazumichi Imai ,  Eiichiro Takada ,  Sadamitsu Aso

IPC: F16K27/00 ,  C12M1/00 ,  B01L3/00 ,  F16K7/04 ,  F16K7/06

Abstract: According to the present invention, a problem of closed systems, namely minimizing the number of electromagnetic valves required to control a plurality of flow paths, can be addressed, and thus a low-cost cell culture device can be achieved. In this flow-path control method for X number of flow paths satisfying X≤2N, the X number of flow paths are selected by using N number of valves to simultaneously and selectively control the opening and closing of the plurality of flow paths.

公开(公告)号：US10453192B2

公开(公告)日：2019-10-22

申请号：US15556546

申请日：2016-01-28

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Hideharu Hattori ,  Yasuki Kakishita ,  Kenko Uchida ,  Sadamitsu Aso ,  Fumiaki Hamazato

IPC: G06K9/00 ,  G06T7/00 ,  G01N33/483 ,  G06K9/46 ,  G06K9/62

Abstract: The present invention realizes determination of tissues and cells from an image by calculating feature values of a deformed degree of the cells even in a case where the tissues or the cells have various shapes. An image diagnosis support apparatus according to the present invention executes processing of inputting an image of cells, processing of extracting the feature values of a plurality of direction components from a target image of the processing, processing of determining whether or not the image corresponds to one-classification by using the plurality of feature values, and processing of determining whether or not the determination processing is finished with respect to all of the classifications set in advance (FIG. 1).

公开(公告)号：US10266801B2

公开(公告)日：2019-04-23

申请号：US15106945

申请日：2014-12-24

Applicant: Hitachi High-Technologies Corporation

Inventor： Akihiro Shimase ,  Kazumichi Imai ,  Eiichiro Takada ,  Sadamitsu Aso

IPC: C12M1/00 ,  C12M3/06

Abstract: According to the present invention, a simple structure can be used to achieve reliable liquid delivery with no residual air, and simple attachment/detachment of a culture vessel, and thus a closed-system cell culture device exhibiting high reliability can be constructed at low cost. In the present invention, a liquid is supplied or discharged while a culture vessel is in an inclined state. The culture vessel is provided with two flow paths, namely an intake flow path and a discharge flow path, which connect a culture chamber and a connection member. Points where the flow paths join with the culture chamber are respectively configured as an intake port and a discharge port. The discharge port is provided in the position nearest to the axis of inclination of the culture vessel. The intake port is provided in a plane projected from a vertical plane including the aforementioned axis of inclination.

公开(公告)号：US10241062B2

公开(公告)日：2019-03-26

申请号：US14774367

申请日：2014-01-22

Applicant: Hitachi High-Technologies Corporation

Inventor： Yusuke Ominami ,  Mitsugu Sato ,  Kenko Uchida ,  Sadamitsu Aso ,  Taku Sakazume ,  Hideo Morishita ,  Sukehiro Ito ,  Takashi Ohshima

IPC: H01J37/20 ,  H01J37/28 ,  H01J37/244 ,  G01N23/22 ,  G01N23/2251 ,  G01N23/2204

Abstract: The purpose of the present invention is to eliminate the effort in placement and extraction of samples in observations using transmitted charged particles. A charged particle beam device (601) is characterized by having: a charged particle optical lens tube that irradiates a sample (6) with a primary charged particle beam; a sample stage on which a light emitting member (500) that emits light because of charged particles that have come by transmission internally in the sample (6) or scattering therefrom or a sample platform (600) having the light emitting member (500) is attachably and detachably disposed; and a detector (503) that detects the light emitted by the light emitting member.

公开(公告)号：US11676268B2

公开(公告)日：2023-06-13

申请号：US16603445

申请日：2018-02-26

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Hideharu Hattori ,  Yasuki Kakishita ,  Kenko Uchida ,  Sadamitsu Aso ,  Toshinari Sakurai

IPC: G06T7/00 ,  G06V10/40 ,  G06V10/764 ,  G06V10/82 ,  G06V10/44 ,  G06V20/69

CPC classification number: G06T7/0012 ,  G06T7/0014 ,  G06V10/454 ,  G06V10/764 ,  G06V10/82 ,  G06V20/69 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30096

Abstract: An image diagnosis assisting apparatus according to the present invention executes: processing of inputting an image of a tissue or cell; processing of extracting a feature amount of a tissue or cell from a processing target image; processing of extracting a feature amount of a tissue or cell from an image having a component different from that of the target image; and processing of determining presence or absence of a lesion and lesion probability for each of the target images by using a plurality of the feature amounts.

公开(公告)号：US11176668B2

公开(公告)日：2021-11-16

申请号：US16323808

申请日：2017-08-28

Applicant: Hitachi High-Technologies Corporation

Inventor： Yasuki Kakishita ,  Hideharu Hattori ,  Kenko Uchida ,  Sadamitsu Aso ,  Toshinari Sakurai

IPC: G06T7/00 ,  A61B5/00 ,  G16H30/00 ,  G06T7/11 ,  G06K9/00

Abstract: Reaction values indicating based on which areas in an input image a plurality of identification results representing the states of cells or tissues are judged are calculated, and differences between a plurality of reaction values are obtained to thereby calculate the degree to which cells or tissues in an area of interest likely are in each state. Thereby, each state of cells or tissues can be visualized at an accurate position in an area of interest; therefore, diagnostic assistance to pathologist becomes possible.