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公开(公告)号：US20140377132A1
公开(公告)日：2014-12-25
申请号：US14364227
申请日：2012-11-27
IPC分类号： G01N35/10
CPC分类号： G01N35/1004
摘要： An automatic analyzer includes a sample dispensing unit that dispenses a sample into a reaction vessel, a reagent dispensing unit that dispenses a reagent, a sample nozzle cleaning tank that cleans a nozzle of the sample dispensing unit, a reagent nozzle cleaning tank that cleans a nozzle of the reagent dispensing unit, a compressor that supplies compressed air, and cleaning water supply means that supplies cleaning water. The tanks each have an upper vent that allows a nozzle to have access for cleaning, a lower vent that drains the cleaning water, a cleaning water jet port that sprays the cleaning water to the nozzle, and a compressed air jet port that removes residual cleaning water left on the nozzle. The lower vent has an opening area wider than that of the upper vent.
摘要翻译： 一种自动分析装置，其特征在于，包括将样品分配到反应容器中的样本分注单元，分注试剂的试剂分注单元，清洗样品分注单元的喷嘴的样品喷嘴清洗槽，清洗喷嘴的试剂喷嘴清洗槽 的试剂分注单元，提供压缩空气的压缩机，以及用于提供清洁水的清洁供水装置。 每个罐都具有允许喷嘴进入清洁的上部通风口，排出清洁水的下部通风口，将清洁水喷射到喷嘴的清洁水喷射口以及去除残余清洁的压缩空气喷射口 水留在喷嘴上。 下部通风口的开口面积大于上部通风口的开口面积。
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公开(公告)号：US10266801B2
公开(公告)日：2019-04-23
申请号：US15106945
申请日：2014-12-24
摘要： According to the present invention, a simple structure can be used to achieve reliable liquid delivery with no residual air, and simple attachment/detachment of a culture vessel, and thus a closed-system cell culture device exhibiting high reliability can be constructed at low cost. In the present invention, a liquid is supplied or discharged while a culture vessel is in an inclined state. The culture vessel is provided with two flow paths, namely an intake flow path and a discharge flow path, which connect a culture chamber and a connection member. Points where the flow paths join with the culture chamber are respectively configured as an intake port and a discharge port. The discharge port is provided in the position nearest to the axis of inclination of the culture vessel. The intake port is provided in a plane projected from a vertical plane including the aforementioned axis of inclination.
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公开(公告)号：US09862921B2
公开(公告)日：2018-01-09
申请号：US14896123
申请日：2014-06-17
发明人： Naoko Senda , Yoko Inoue , Hiroaki Nakagawa , Akihiro Shimase , Ryota Nakajima , Kazumichi Imai , Shizu Takeda
CPC分类号： C12M41/46 , C12M41/48 , G01N33/5005 , G01N33/6806 , G01N33/6812
摘要： There is provided a method for noninvasively evaluating the cell state (proliferation, multi-layering, and differentiation) of a cell sheet as a mimic tissue at the time of culturing the cell sheet. The method is characterized in that an analysis of an amino acid is conducted with the use of the culture supernatant of a cell sheet to monitor a change in the concentration of any amino acid selected from a group of 5-species of amino acids (Ile, Val, Ser, Leu, and Ala), thereby making a determination.
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公开(公告)号：US10208277B2
公开(公告)日：2019-02-19
申请号：US15107548
申请日：2014-12-24
摘要： According to the present invention, a problem of closed systems, namely minimizing the number of electromagnetic valves required to control a plurality of flow paths, can be addressed, and thus a low-cost cell culture device can be achieved. In this flow-path control method for X number of flow paths satisfying X≤2N, the X number of flow paths are selected by using N number of valves to simultaneously and selectively control the opening and closing of the plurality of flow paths.
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公开(公告)号：US10456767B2
公开(公告)日：2019-10-29
申请号：US15518041
申请日：2014-10-22
IPC分类号： B01J19/00 , C12M1/34 , C12M1/36 , C12M1/00 , G01N15/06 , G01N21/53 , G01N33/483 , G01N33/50 , G01N15/00
摘要： A cytometric mechanism includes: a flow path through which a cell suspension is made to flow; a liquid drive unit for sending the cell suspension which is in the flow path; and a computation unit for irradiating, with irradiation light from a light source, a cell suspension flowing through a flow cell, and for finding a cell survival rate in the cell suspension on the basis of a resulting forward scattered light intensity and transmittance and/or side scattered light intensity. The invention is provided with a calibration curve database for storing, in advance, respective calibration curves indicative of a relationship between viable cell concentration and forward scattered light intensity, a relationship between dead cell concentration and the transmittance, and a relationship between a cell survival rate and the side scattered light intensity.
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公开(公告)号：US10138456B2
公开(公告)日：2018-11-27
申请号：US15327208
申请日：2015-07-08
发明人： Akihiro Shimase , Kazumichi Imai , Sadamitsu Aso , Eiichiro Takada , Masako Kawarai , Toshinari Sakurai
摘要： A device which automatically performs a step in which expanded and cultured cells are diluted to a desired cell concentration and re-inoculated using a cell-concentration adjustment device having an inlet for taking in a cell suspension; an outlet for discharging a diluted cell suspension; and a flow path which is provided between the inlet and the outlet and is capable of holding a cell suspension, the flow path being provided with: a liquid delivery pump for causing a cell suspension inside to flow; a cell-concentration measurement instrument for collecting data related to a cell concentration per unit amount of the cell suspension; and a dilution-liquid container for holding a dilution liquid which is supplied to the flow path to dilute the cell suspension. The device further includes a control unit for controlling at least the liquid delivery pump on the basis of the data obtained by the cell-concentration measurement instrument, wherein the control unit determines, on the basis of the data obtained by the cell-concentration measurement instrument, an amount of the dilution liquid required to bring the cell concentration to the desired concentration, and drives the liquid delivery pump so as to take in the required amount of the dilution liquid into the flow path and mix the cell suspension and the dilution liquid.
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