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    COMPOSITIONS AND METHODS RELATED TO AN ADENOVIRAL TRANS-COMPLEMENTING CELL LINE
    4.
    发明申请
    COMPOSITIONS AND METHODS RELATED TO AN ADENOVIRAL TRANS-COMPLEMENTING CELL LINE 审中-公开
    与腺病毒转导细胞系相关的组合物和方法

    公开(公告)号：US20090253184A1

    公开(公告)日：2009-10-08

    申请号：US12358662

    申请日：2009-01-23

    申请人： Peter Clarke ,  Shuyuan Zhang ,  Hai Pham ,  Joe Senesac

    发明人： Peter Clarke ,  Shuyuan Zhang ,  Hai Pham ,  Joe Senesac

    IPC分类号： C12N15/64 ,  C07H21/00 ,  C12N5/00

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10352

    摘要： Embodiments of the invention include E1 expressing cell lines that can be used in a variety of methods for production of an E1 defective adenovirus. In certain aspects a cell of the invention can be adapted to various culture conditions, e.g., suspension culture in serum free medium. In a further aspect, the cell lines allow isolation and subculture of E1-deleted recombinant adenoviruses in an environment free of replication competent adenovirus (RCA).

    摘要翻译： 本发明的实施方案包括可用于生产E1缺陷型腺病毒的各种方法中的E1表达细胞系。 在某些方面，本发明的细胞可以适应于各种培养条件，例如在无血清培养基中的悬浮培养。 在另一方面，细胞系允许在无复制感受态腺病毒（RCA）的环境中分离和传代E1缺失的重组腺病毒。

    Methods and compositions for the production of adenoviral vectors
    7.
    发明授权
    Methods and compositions for the production of adenoviral vectors 有权
    用于产生腺病毒载体的方法和组合物

    公开(公告)号：US07419808B2

    公开(公告)日：2008-09-02

    申请号：US11079986

    申请日：2005-03-15

    申请人： Shuyuan Zhang ,  Hai Pham

    发明人： Shuyuan Zhang ,  Hai Pham

    IPC分类号： C12P21/04 ,  A61K39/00 ,  A61K39/235 ,  C12N13/00 ,  A61K39/193

    CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10351

    摘要： The present invention addresses the need to improve the yield of adenovirus when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of infection temperatures lower than 37° C. in a cell culture system results in improved yields of adenovirus. In addition, it has been demonstrated that when host cells are grow in a bioreactor, initiating adenovirus infection by diluting the host cells with fresh media and adenovirus results in improved yield of adenovirus. Methods of adenoviral production and purification using infection temperatures less than 37° C. are disclosed. Methods of adenoviral production and purification wherein the host cells are grown in a bioreactor and adenovirus infection is initiated by diluting the host cells with fresh media and adenovirus are also disclosed.

    摘要翻译： 本发明解决了在细胞培养系统中生长时提高腺病毒产量的需要。 特别地，已经证明，对于腺病毒，在细胞培养系统中使用低于37℃的感染温度导致腺病毒的产量提高。 此外，已经证明当宿主细胞在生物反应器中生长时，通过用新鲜培养基和腺病毒稀释宿主细胞来引发腺病毒感染导致腺病毒的产量提高。 公开了使用感染温度低于37℃的腺病毒产生和纯化方法。 还公开了通过用新鲜培养基和腺病毒稀释宿主细胞来引发宿主细胞在生物反应器中生长和腺病毒感染的腺病毒产生和纯化的方法。

    Methods and compositions for the production of adenoviral vectors
    8.
    发明申请
    Methods and compositions for the production of adenoviral vectors 有权
    用于产生腺病毒载体的方法和组合物

    公开(公告)号：US20050158283A1

    公开(公告)日：2005-07-21

    申请号：US11079986

    申请日：2005-03-15

    申请人： Shuyuan Zhang ,  Hai Pham

    发明人： Shuyuan Zhang ,  Hai Pham

    IPC分类号： C12N7/01 ,  C12N7/02 ,  C12N15/861 ,  A61K48/00 ,  C12N7/00

    CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10351

    摘要： The present invention addresses the need to improve the yield of adenovirus when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of infection temperatures lower than 37° C. in a cell culture system results in improved yields of adenovirus. In addition, it has been demonstrated that when host cells are grow in a bioreactor, initiating adenovirus infection by diluting the host cells with fresh media and adenovirus results in improved yield of adenovirus. Methods of adenoviral production and purification using infection temperatures less than 37° C. are disclosed. Methods of adenoviral production and purification wherein the host cells are grown in a bioreactor and adenovirus infection is initiated by diluting the host cells with fresh media and adenovirus are also disclosed.

    摘要翻译： 本发明解决了在细胞培养系统中生长时提高腺病毒产量的需要。 特别地，已经证明，对于腺病毒，在细胞培养系统中使用低于37℃的感染温度导致腺病毒的产量提高。 此外，已经证明当宿主细胞在生物反应器中生长时，通过用新鲜培养基和腺病毒稀释宿主细胞来引发腺病毒感染导致腺病毒的产量提高。 公开了使用感染温度低于37℃的腺病毒产生和纯化方法。 还公开了通过用新鲜培养基和腺病毒稀释宿主细胞来引发宿主细胞在生物反应器中生长和腺病毒感染的腺病毒产生和纯化的方法。