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公开(公告)号：US07056740B2
公开(公告)日：2006-06-06
申请号：US10356708
申请日：2003-01-31
申请人： Hal S. Padgett , Andrew A. Vaewhongs , Fakhrieh S. Vojdani , Mark L. Smith , John A. Lindbo , Wayne P. Fitzmaurice
发明人： Hal S. Padgett , Andrew A. Vaewhongs , Fakhrieh S. Vojdani , Mark L. Smith , John A. Lindbo , Wayne P. Fitzmaurice
IPC分类号： C12N15/00
CPC分类号： C12N15/102 , C07K14/005 , C12N9/22 , C12N15/1027 , C12N15/8203 , C12N15/8257 , C12N2770/00022
摘要： We describe here restriction endonucleases and their uses. Restriction endonucleases are useful in finding single nucleotide polymorphisms. They are also useful in an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences.
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公开(公告)号：US20110111413A1
公开(公告)日：2011-05-12
申请号：US12952209
申请日：2010-11-23
IPC分类号： C12Q1/68
CPC分类号： C12N15/102 , C12N15/1027
摘要： The present invention relates to codon optimization utilizing DNA shuffling. A method of producing gene sequences optimized for a desired functional property is described involving synthesizing a library of parental codon variant genes encoding some or all codon choices at some or all amino acid positions of a gene, reassorting the variant codons among the parental codon variant genes using DNA shuffling thereby forming progeny codon variant genes, expressing the progeny codon variant genes in a host; and screening or selecting for progeny codon variant genes encoding a desired functional property.
摘要翻译： 本发明涉及使用DNA改组的密码子优化。 描述了产生针对所需功能性质优化的基因序列的方法，其涉及在基因的某些或所有氨基酸位置合成编码一些或所有密码子选择的亲代密码子变体基因的文库，重排亲代密码子变体基因中的变体密码子 使用DNA改组从而形成子代密码子变体基因，在宿主中表达后代密码子变体基因; 以及筛选或选择编码期望功能特性的后代密码子变体基因。
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公开(公告)号：US07838219B2
公开(公告)日：2010-11-23
申请号：US10637758
申请日：2003-08-08
CPC分类号： C12N15/1027 , C12N15/102
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
摘要翻译： 我们在这里描述了增加异源双链多核苷酸序列中的互补性的体外方法。 该方法使用相反链的退火以形成具有错配的多核苷酸双链体。 将异源双链多核苷酸与有效量的具有链切割活性，3'至5'核酸外切酶活性和聚合酶活性的酶组合，并且允许足够的时间在异源双链体内增加互补百分比。 并非所有的异源双链多核苷酸都必然具有解决互补性的所有错配。 任选地连接得到的多核苷酸。 产生几种变异多核苷酸。 在相对链中的任一个在另一条链中模板记录的位点，异源双链多核苷酸序列的所得百分比互补性增加。 在退火异源链之前，母体多核苷酸不需要切割成片段。 因此，不需要重新组装。
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4.发明申请SELECTION AND CHARACTERIZATION OF NOVEL PLANT-DERIVED RECOMBINANT HUMAN INTERFERONS WITH BROAD SPECTRUM ACTIVITY 有权新型植物衍生重组人类干扰素与广谱光谱活性的选择和表征公开(公告)号：US20120302733A1
公开(公告)日：2012-11-29
申请号：US13478330
申请日：2012-05-23
IPC分类号： C07K14/555
CPC分类号： C07K14/555 , C07K14/56 , C07K2319/04 , C07K2319/21 , C12N15/8257
摘要： Methods to derive novel hybrid type 1 interferons that are broadly active against highly pathogenic viruses of biodefense significance are described. Libraries of hybrid interferon genes were produced using gene shuffling, the proteins were expressed, and screened for activity against viruses of interest. Sequences of several broadly active hybrid interferons are described.
摘要翻译： 描述了衍生对具有生物防御重要性的高致病性病毒广泛有效的新型杂合1型干扰素的方法。 使用基因改组产生杂交干扰素基因的文库，对蛋白进行表达，筛选出针对目的病毒的活性。 描述了几种广泛活性的混合干扰素的序列。
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公开(公告)号：US07888475B2
公开(公告)日：2011-02-15
申请号：US12560391
申请日：2009-09-15
CPC分类号： C07K14/555 , C12N2710/24011
摘要： Herein is described a system to combat poxvirus infection wherein antagonists are developed that bind the soluble cytokine receptor but have no significant biological activity in the host, effectively blocking the virus-mediated suppressor of interferon function, thereby permitting the host's own cytokines to stimulate an antiviral response. Alternatively, interferon molecules can be developed that retain biological activity on their native receptors but fail to bind the viral cytokine binding protein, thereby circumventing this virus immune modulation mechanism.
摘要翻译： 本文描述了一种防止痘病毒感染的系统，其中开发出拮抗剂结合可溶性细胞因子受体但在宿主中没有显着生物学活性的系统，有效阻断病毒介导的干扰素功能抑制因子，由此允许宿主自身的细胞因子刺激抗病毒 响应。 或者，可以开发干扰素分子，其保留对其天然受体的生物活性，但不能结合病毒细胞因子结合蛋白，从而规避该病毒免疫调节机制。
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6.发明授权Selection and characterization of novel plant-derived recombinant human interferons with broad spectrum activity 有权具有广谱活性的新型植物来源的重组人类干扰素的选择和表征公开(公告)号：US08729236B2
公开(公告)日：2014-05-20
申请号：US13478330
申请日：2012-05-23
IPC分类号： C07K14/52
CPC分类号： C07K14/555 , C07K14/56 , C07K2319/04 , C07K2319/21 , C12N15/8257
摘要： Methods to derive novel hybrid type 1 interferons that are broadly active against highly pathogenic viruses of biodefense significance are described. Libraries of hybrid interferon genes were produced using gene shuffling, the proteins were expressed, and screened for activity against viruses of interest. Sequences of several broadly active hybrid interferons are described.
摘要翻译： 描述了衍生对具有生物防御重要性的高致病性病毒广泛有效的新型杂合1型干扰素的方法。 使用基因改组产生杂交干扰素基因的文库，对蛋白进行表达，筛选出针对目的病毒的活性。 描述了几种广泛活性的混合干扰素的序列。
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公开(公告)号：US20100121030A1
公开(公告)日：2010-05-13
申请号：US12560391
申请日：2009-09-15
CPC分类号： C07K14/555 , C12N2710/24011
摘要： Herein is described a system to combat poxvirus infection wherein antagonists are developed that bind the soluble cytokine receptor but have no significant biological activity in the host, effectively blocking the virus-mediated suppressor of interferon function, thereby permitting the host's own cytokines to stimulate an antiviral response. Alternatively, interferon molecules can be developed that retain biological activity on their native receptors but fail to bind the viral cytokine binding protein, thereby circumventing this virus immune modulation mechanism.
摘要翻译： 本文描述了一种防止痘病毒感染的系统，其中开发出拮抗剂结合可溶性细胞因子受体但在宿主中没有显着生物学活性的系统，有效阻断病毒介导的干扰素功能抑制因子，由此允许宿主自身的细胞因子刺激抗病毒 响应。 或者，可以开发干扰素分子，其保留对其天然受体的生物活性，但不能结合病毒细胞因子结合蛋白，从而规避该病毒免疫调节机制。
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公开(公告)号：US07078211B2
公开(公告)日：2006-07-18
申请号：US10211079
申请日：2002-08-01
CPC分类号： C12N15/102 , C07K14/005 , C12N9/22 , C12N15/1027 , C12N15/8203 , C12N15/8257 , C12N2770/00022
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
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9.发明授权公开(公告)号：US07273739B2
公开(公告)日：2007-09-25
申请号：US11417448
申请日：2006-05-03
CPC分类号： C12N15/102 , C07K14/005 , C12N9/22 , C12N15/1027 , C12N15/8203 , C12N15/8257 , C12N2770/00022
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
摘要翻译： 我们在这里描述了增加异源双链多核苷酸序列中的互补性的体外方法。 该方法使用相反链的退火以形成具有错配的多核苷酸双链体。 将异源双链多核苷酸与有效量的具有链切割活性，3'至5'核酸外切酶活性和聚合酶活性的酶组合，并且允许足够的时间在异源双链体内增加互补百分比。 并非所有的异源双链多核苷酸都必然具有解决互补性的所有错配。 任选地连接得到的多核苷酸。 产生几种变异多核苷酸。 在相对链中的任一个在另一条链中模板记录的位点，异源双链多核苷酸序列的所得百分比互补性增加。 在退火异源链之前，母体多核苷酸不需要切割成片段。 因此，不需要重新组装。
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10.发明申请NUCLEIC ACID MOLECULES ENCODING MISMATCH ENDONUCLEASES AND METHODS OF USE THEREOF 审中-公开编码缺失缺失突变体的核酸分子及其使用方法公开(公告)号：US20080145913A1
公开(公告)日：2008-06-19
申请号：US11860465
申请日：2007-09-24
IPC分类号： C12N9/18
CPC分类号： C12N15/102 , C07K14/005 , C12N9/22 , C12N15/1027 , C12N15/8203 , C12N15/8257 , C12N2770/00022
摘要： We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. Also described are mismatch endonucleases suitable for use in the process.
摘要翻译： 我们在这里描述了增加异源双链多核苷酸序列中的互补性的体外方法。 该方法使用相反链的退火以形成具有错配的多核苷酸双链体。 将异源双链多核苷酸与有效量的具有链切割活性，3'至5'核酸外切酶活性和聚合酶活性的酶组合，并且允许足够的时间在异源双链体内增加互补百分比。 并非所有的异源双链多核苷酸都必然具有解决互补性的所有错配。 任选地连接得到的多核苷酸。 产生几种变异多核苷酸。 在相对链中的任一个在另一条链中模板记录的位点，异源双链多核苷酸序列的所得百分比互补性增加。 还描述了适用于该方法的不匹配核酸内切酶。
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