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    Process for the isolation and characterization of a gene enzyme system for inactivation of the herbicide phenmedipham and transfer of the gene into plants to produce herbicide-tolerant plants
    2.
    发明授权
    Process for the isolation and characterization of a gene enzyme system for inactivation of the herbicide phenmedipham and transfer of the gene into plants to produce herbicide-tolerant plants 失效
    用于分离和表征用于灭活除草剂phenmedipham的基因酶系统和将基因转移到植物中以产生除草剂耐受性植物的方法

    公开(公告)号：US5948670A

    公开(公告)日：1999-09-07

    申请号：US448128

    申请日：1995-05-23

    申请人： Hans-Dieter Pohlenz ,  Werner Boidol ,  Wolfgang Streber

    发明人： Hans-Dieter Pohlenz ,  Werner Boidol ,  Wolfgang Streber

    IPC分类号： C12N9/80 ,  C12N15/31 ,  C12N1/20 ,  C12N15/52 ,  C12P21/00

    CPC分类号： C12N9/80 ,  Y10S435/83

    摘要： A process for the isolation and characterization of a gene enzyme system for the inactivation of the herbicide phenmedipham, wherein the enzyme is a carbamate hydrolase of Arthrobacter oxidans, which is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. This process includes the isolation of the carbamate hydrolase, the identification of the amino acid sequence of two BrCN cleavage peptides of the carbamate hydrolase, the synthesis of oligonucleotides for specific determination of the carbamate hydrolase sequence by hybridization and identification of the coding region, cloning and specifying the nucleotide sequence of the carbamate hydrolase gene from Arthrobacter oxidans.

    摘要翻译： 用于分离和表征用于灭活除草剂phenmedipham的基因酶系统的方法，其中所述酶是氧化亚铜节杆菌的氨基甲酸酯水解酶，其负责所述苯二酚的苯环之间的氨基甲酸酯键的裂解。 该方法包括氨基甲酸酯水解酶的分离，氨基甲酸酯水解酶的两个BrCN切割肽的氨基酸序列的鉴定，用于通过杂交和鉴定编码区特异性测定氨基甲酸酯水解酶序列的寡核苷酸的合成，克隆和 指定氧化节杆菌的氨基甲酸酯水解酶基因的核苷酸序列。

    Process for the isolation and characterisation of a gene enzyme system for inactivation of the herbicide phenmedipham and transfer of the gene into plants
    3.
    发明授权
    Process for the isolation and characterisation of a gene enzyme system for inactivation of the herbicide phenmedipham and transfer of the gene into plants 失效
    用于分离和表征用于灭活除草剂phenmedipham和将基因转移到植物中的基因酶系统的方法

    公开(公告)号：US5543306A

    公开(公告)日：1996-08-06

    申请号：US196361

    申请日：1994-02-14

    申请人： Hans-Dieter Pohlenz ,  Werner Boidol ,  Wolfgang Streber

    发明人： Hans-Dieter Pohlenz ,  Werner Boidol ,  Wolfgang Streber

    IPC分类号： C12N9/80 ,  C12N15/31 ,  C12N15/82 ,  C12N15/55 ,  C12N15/67

    CPC分类号： C12N9/80 ,  Y10S435/83

    摘要： A process for the isolation and characterization of a gene enzyme system for the inactivation of the herbicide phenmedipham, wherein the enzyme is a carbamate hydrolase of Arthrobacter oxidans, which is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. This process includes the isolation of the carbamate hydrolase, the identification of the amino acid sequence of two BrCN cleavage peptides of the carbamate hydrolase, the synthesis of oligonucleotides for specific determination of the carbamate hydrolase sequence by hybridization and identification of the coding region, cloning and specifying the nucleotide sequence of the carbamate hydrolase gene from Arthrobacter oxidans.

    摘要翻译： 用于分离和表征用于灭活除草剂phenmedipham的基因酶系统的方法，其中所述酶是氧化亚铜节杆菌的氨基甲酸酯水解酶，其负责所述苯二酚的苯环之间的氨基甲酸酯键的裂解。 该方法包括氨基甲酸酯水解酶的分离，氨基甲酸酯水解酶的两个BrCN切割肽的氨基酸序列的鉴定，用于通过杂交和鉴定编码区特异性测定氨基甲酸酯水解酶序列的寡核苷酸的合成，克隆和 指定氧化节杆菌的氨基甲酸酯水解酶基因的核苷酸序列。

    Herbicide-tolerant plants expressing carbamate hydrolase
    4.
    发明授权
    Herbicide-tolerant plants expressing carbamate hydrolase 失效
    表达氨基甲酸酯水解酶的除草剂耐受性植物

    公开(公告)号：US5347076A

    公开(公告)日：1994-09-13

    申请号：US615448

    申请日：1990-11-19

    申请人： Hans-Dieter Pohlenz ,  Werner Boidol ,  Wolfgang Streber

    发明人： Hans-Dieter Pohlenz ,  Werner Boidol ,  Wolfgang Streber

    IPC分类号： C12N9/80 ,  C12N15/31 ,  A01H4/00 ,  C12N15/82

    CPC分类号： C12N9/80 ,  Y10S435/83

    摘要： A process for the isolation and characterization of a gene enzyme system for the inactivation of the herbicide phenmedipham, wherein the enzyme is a carbamate hydrolase of Arthrobacter oxidans, which is responsible for the cleavage of the carbamate bond between the benzene rings of phenmedipham. This process includes the isolation of the carbamate hydrolase, the identification of the amino acid sequence of two BrCN cleavage peptides of the carbamate hydrolase, the synthesis of oligonucleotides for specific determination of the carbamate hydrolase sequence by hybridization and identification of the coding region, cloning and specifying the nucleotide sequence of the carbamate hydrolase gene from Arthrobacter oxidans.Plants are transformed with recombinant genes coding for the carbamate hydrolase and transgenic plants which are tolerant to the herbicide are produced.

    摘要翻译： 用于分离和表征用于灭活除草剂phenmedipham的基因酶系统的方法，其中所述酶是氧化亚铜节杆菌的氨基甲酸酯水解酶，其负责所述苯二酚的苯环之间的氨基甲酸酯键的裂解。 该方法包括氨基甲酸酯水解酶的分离，氨基甲酸酯水解酶的两个BrCN切割肽的氨基酸序列的鉴定，用于通过杂交和鉴定编码区特异性测定氨基甲酸酯水解酶序列的寡核苷酸的合成，克隆和 指定氧化节杆菌的氨基甲酸酯水解酶基因的核苷酸序列。 用编码氨基甲酸酯水解酶的重组基因转化植物，并产生耐除草剂的转基因植物。

    DNA sequences encoding the subunit CHLD of plant magnesium chelatases, and determining the activity of plant magnesium chelatases
    7.
    发明申请
    DNA sequences encoding the subunit CHLD of plant magnesium chelatases, and determining the activity of plant magnesium chelatases 审中-公开
    编码植物镁螯合酶亚基CHLD的DNA序列，并确定植物镁螯合酶的活性

    公开(公告)号：US20050102712A1

    公开(公告)日：2005-05-12

    申请号：US10637763

    申请日：2003-08-08

    申请人： Bernhard Grimm ,  Jutta Papenbrock ,  Frank Hanel ,  Susanna Grafe ,  Frank Schmidt ,  Wolfgang Streber

    发明人： Bernhard Grimm ,  Jutta Papenbrock ,  Frank Hanel ,  Susanna Grafe ,  Frank Schmidt ,  Wolfgang Streber

    IPC分类号： A01H5/00 ,  C07K16/16 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N15/09 ,  C12N15/82 ,  C12Q1/68 ,  A01H1/00 ,  C12Q1/48

    CPC分类号： C12N9/00 ,  C12N15/8269 ,  C12N15/8274

    摘要： The present invention relates to a nucleic acid molecule which encodes a protein with the function of a plant Mg chelatase subunit CHLD or an active fragment thereof; a protein which has the function of a plant Mg chelatase subunit CHLD or an active fragment thereof, preferably a recombinant protein; a method of determining the interaction of plant Mg chelatase subunits, in which a host cell is transformed with a DNA sequence as claimed in one or more of claims 1 to 3 and at least with one DNA sequence encoding a further subunit of Mg chelatase in such a manner that the interaction of the Mg chelatase gene products leads to a directly or indirectly, qualitatively or quantitatively measurable signal, preferably by activating a marker gene, and transgenic plants, transgenic plant cells, transgenic plant organs, transgenic plant seeds, transgenic propagation material comprising an abovementioned nucleic acid molecule.

    摘要翻译： 本发明涉及编码具有植物Mg螯合酶亚基CHLD或其活性片段的功能的蛋白质的核酸分子; 具有植物Mg螯合酶亚单位CHLD或其活性片段，优选重组蛋白质的功能的蛋白质; 确定植物Mg螯合酶亚基的相互作用的方法，其中宿主细胞用如权利要求1至3中的一项或多项所述的DNA序列转化，并且至少编码一种编码其中的Mg螯合酶亚单位的DNA序列 Mg螯合酶基因产物的相互作用导致直接或间接，定性或定量可信号的信号，优选通过激活标记基因和转基因植物，转基因植物细胞，转基因植物器官，转基因植物种子，转基因繁殖材料 包含上述核酸分子。

    DNA sequences encoding for subunit CHLD of plant magnesium chelatases and determining the activity of plant magnesium chelatases
    8.
    发明授权
    DNA sequences encoding for subunit CHLD of plant magnesium chelatases and determining the activity of plant magnesium chelatases 失效
    编码植物镁螯合酶亚基CHLD的DNA序列，并确定植物镁螯合酶的活性

    公开(公告)号：US06831207B1

    公开(公告)日：2004-12-14

    申请号：US09403463

    申请日：1999-10-21

    申请人： Bernhard Grimm ,  Jutta Papenbrock ,  Frank Hänel ,  Susanna Gräfe ,  Frank Schmidt ,  Wolfgang Streber

    发明人： Bernhard Grimm ,  Jutta Papenbrock ,  Frank Hänel ,  Susanna Gräfe ,  Frank Schmidt ,  Wolfgang Streber

    IPC分类号： A01H500

    CPC分类号： C12N9/00 ,  C12N15/8269 ,  C12N15/8274

    摘要： The present invention relates to a nucleic acid molecule which encodes a protein with the function of a plant Mg chelatase subunit CHLD or an active fragment thereof; a protein which has the function of a plant Mg chelatase subunit CHLD or an active fragment thereof, preferably a recombinant protein; a method of determining the interaction of plant Mg chelatase subunits, in which a host cell is transformed with a DNA sequence as claimed in one or more of claims 1 to 3 and at least with one DNA sequence encoding a further subunit of Mg chelatase in such a manner that the interaction of the Mg chelatase gene products leads to a directly or indirectly, qualitatively or quantitatively measurable signal, preferably by activating a marker gene, and transgenic plants, transgenic plant cells, transgenic plant organs, transgenic plant seeds, transgenic propagation material comprising an abovementioned nucleic acid molecule.

    摘要翻译： 本发明涉及编码具有植物Mg螯合酶亚基CHLD或其活性片段的功能的蛋白质的核酸分子; 具有植物Mg螯合酶亚单位CHLD或其活性片段，优选重组蛋白质的功能的蛋白质; 确定植物Mg螯合酶亚基的相互作用的方法，其中宿主细胞用如权利要求1至3中的一项或多项所述的DNA序列转化，并且至少在编码另一个Mg螯合酶亚单位的DNA序列中 Mg螯合酶基因产物的相互作用导致直接或间接，定性或定量可信号的信号，优选通过激活标记基因和转基因植物，转基因植物细胞，转基因植物器官，转基因植物种子，转基因繁殖材料 包含上述核酸分子。