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1.发明授权Recombinant kinase from insect cells for the synthesis of nucleoside monophosphates 有权来自昆虫细胞的重组激酶用于合成核苷单磷酸公开(公告)号：US07625735B2
公开(公告)日：2009-12-01
申请号：US10680635
申请日：2003-10-07
CPC分类号： C12N9/1205
摘要： A recombinant Drosophila melanogaster deoxynucleoside kinase that remains stable during the synthesis of nucleoside monophosphate without the addition of stabilizing SH reagents or stabilizing proteins, and that accepts all four natural deoxynucleotides is provided. In addition, the invention concerns DNA sequences, vectors, transformed cells, a method for production of the recombinant kinase as well as its use for preparing nucleoside monophosphates.
摘要翻译： 提供了一种重组黑腹果蝇脱氧核苷激酶，其在合成核苷单磷酸酯期间保持稳定，而不添加稳定的SH试剂或稳定蛋白，并且接受所有四种天然脱氧核苷酸。 此外，本发明涉及DNA序列，载体，转化细胞，重组激酶的制备方法及其制备核苷单磷酸的用途。
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2.发明申请Deoxynucleoside kinase from insect cells for the synthesis of nucleoside monophosphates 有权来自昆虫细胞的脱氧核苷激酶用于合成核苷单磷酸酯公开(公告)号：US20060252131A1
公开(公告)日：2006-11-09
申请号：US10680635
申请日：2003-10-07
CPC分类号： C12N9/1205
摘要： Recombinant kinase remaining stable during the synthesis of necleoside monophosphate without the addition of stabilizing SH reagents, without stabilizing proteins and accepting all four natural deoxynucleotides, obtainable from insect cells such as e.g. Drosophila Melanogaster. In addition, the invention concerns DNA sequences, vectors, transformed cells, a method for production of the recombinant kinase as well as its use for preparing nucleoside monophosphates.
摘要翻译： 重组激酶在合成核苷酸单磷酸酯期间保持稳定，而不添加稳定的SH试剂，而不稳定蛋白质并接受可从昆虫细胞获得的所有四种天然脱氧核苷酸。 果蝇Melanogaster。 此外，本发明涉及DNA序列，载体，转化细胞，重组激酶的制备方法及其制备核苷单磷酸的用途。
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3.发明授权Method for the production of heterologous polypeptides in transformed yeast cells 失效用于在转化的酵母细胞中产生异源多肽的方法公开(公告)号：US06190883B1
公开(公告)日：2001-02-20
申请号：US09392126
申请日：1999-09-08
IPC分类号： C12P2106
摘要： The invention describes industrial fermentation of a Saccharomyces yeast species for production of a heterologous product encoded by a plasmid or DNA contained in said Saccharomyces yeast species which method utilizes the substrate more efficiently and without fermentative metabolism resulting in formation of ethanol and other unwanted primary products of fermentative activity whereby high yields of the heterologous product are obtained. The Saccharomyces yeast species is preferably a Crabtree negative Saccharomyces species in particular Saccharomyces kluyveri.
摘要翻译： 本发明描述了酿酒酵母物种的工业发酵，用于生产由所述酵母属酵母菌种中含有的质粒或DNA编码的异源产物，该方法利用该底物更有效且无发酵代谢，从而形成乙醇和其它不需要的初级产物 发酵活性，从而获得高产量的异源产物。 酵母属酵母菌种优选是Crabtree阴性酵母菌种，特别是酵母菌（Saccharomyces kluyveri）。
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公开(公告)号：US07666639B2
公开(公告)日：2010-02-23
申请号：US10519395
申请日：2003-06-24
CPC分类号： A61K45/06 , A61K38/00 , C12N9/1205 , C12N9/1211 , C12N15/8261 , C12Y207/01076 , Y02A40/146
摘要： This invention relates to pharmaceutical compositions comprising plant deoxyribonucleoside kinase enzymes (dNK) capable of phosphorylating nucleoside analogs and to medical use of said dNKs. More specifically the invention relates to the medical use of deoxyribonucleoside kinase enzymes derived from (Arabidopsis thaliana), from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa). The invention also relates to methods of sensitizing cells to prodrugs, and to methods of inhibiting pathogenic agents in warm-blooded animals using said plant dNKs.In another aspect the invention relates to plant derived deoxyribonucleoside kinase enzymes provided in isolated form from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa). In further aspects the invention provides polynucleotides encoding the plant dNKs, vector constructs comprising the polynucleotide, packaging cell lines capable of producing said vector, and genetically modified isolated host cells transduced/transfected/-transformed with the vector.
摘要翻译： 本发明涉及包含能够磷酸化核苷类似物的植物脱氧核糖核苷激酶（dNK）和所述dNK的医学用途的药物组合物。 更具体地，本发明涉及来自（拟南芥），来自火炬松（Pinus taeda），番茄（Lycopersicum esculentum），玉米（Zea mays）或来自稻（Oryza sativa）的脱氧核糖核苷激酶的医学用途。 本发明还涉及使细胞对前药敏感的方法，以及使用所述植物dNK在温血动物中抑制病原体的方法。 在另一方面，本发明涉及以番茄（番茄），玉米（Zea mays）或稻（Oryza sativa）分离形式提供的植物衍生的脱氧核糖核苷激酶。 在其它方面，本发明提供了编码植物dNK的多核苷酸，包含多核苷酸的载体构建体，能够产生所述载体的包装细胞系以及用载体转导/转染/转化的遗传修饰的分离的宿主细胞。
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公开(公告)号：US20060230467A1
公开(公告)日：2006-10-12
申请号：US10519395
申请日：2003-06-24
CPC分类号： A61K45/06 , A61K38/00 , C12N9/1205 , C12N9/1211 , C12N15/8261 , C12Y207/01076 , Y02A40/146
摘要： This invention relates to pharmaceutical compositions comprising plant deoxyribonucleoside kinase enzymes (dNK) capable of phosphorylating nucleoside analogs and to medical use of said dNKs. More specifically the invention relates to the medical use of deoxyribonucleoside kinase enzymes derived from (Arabidopsis thaliana), from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa). The invention also relates to methods of sensitising cells to prodrugs, and to methods of inhibiting pathogenic agents in warm-blooded animals using said plant dNKs. In another aspect the invention relates to plant derived deoxyribonucleoside kinase enzymes provided in isolated form from loblolly pine (Pinus taeda), from tomato (Lycopersicum esculentum), from maize (Zea mays) or from rice (Oryza sativa). In further aspects the invention provides polynucleotides encoding the plant dNKs, vector constructs comprising the polynucleotide, packaging cell lines capable of producing said vector, and genetically modified isolated host cells transduced/transfected/-transformed with the vector.
摘要翻译： 本发明涉及包含能够磷酸化核苷类似物的植物脱氧核糖核苷激酶（dNK）和所述dNK的医学用途的药物组合物。 更具体地，本发明涉及来自（拟南芥），来自火炬松（Pinus taeda），番茄（Lycopersicum esculentum），玉米（Zea mays）或来自稻（Oryza sativa）的脱氧核糖核苷激酶的医学用途。 本发明还涉及使细胞对前药敏感的方法，以及使用所述植物dNK在温血动物中抑制病原体的方法。 在另一方面，本发明涉及以番茄（番茄），玉米（Zea mays）或稻（Oryza sativa）分离形式提供的植物衍生的脱氧核糖核苷激酶。 在其它方面，本发明提供了编码植物dNK的多核苷酸，包含多核苷酸的载体构建体，能够产生所述载体的包装细胞系以及用载体转导/转染/转化的遗传修饰的分离的宿主细胞。
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公开(公告)号：US07928206B2
公开(公告)日：2011-04-19
申请号：US10515417
申请日：2003-05-21
CPC分类号： C12N15/8261 , A61K31/7072 , A61K38/45 , A61K45/06 , C12N9/1205 , C12N9/1211 , C12Y207/01076 , Y02A40/146
摘要： This invention relates to novel plant thymidine kinases and their use in gene therapy. More specifically the invention provides novel thymidine kinases derived from tomato.In further aspects the invention provides novel polynucleotides encoding the tomato thymidine kinase of mutant thereof, vector constructs comprising the polynucleotide, host cells carrying the polynucleotide or vector, methods of sensitising cells to prodrugs, methods of inhibiting pathogenic agents in warm-blooded animals, methods for biocontrol of plants, methods of synthesizing monophosphates and pharmaceutical compositions comprising the plant thymidine kinases of the invention.In a preferred embodiment the invention provides a unique combination of a plant thymidine kinase and the nucleoside analog nucleoside analog AZT (3′-azido-3′-deoxythymidine) to treat abnormal cell growth.
摘要翻译： 本发明涉及新型植物胸苷激酶及其在基因治疗中的应用。 更具体地，本发明提供了源自番茄的新型胸苷激酶。 在另外的方面，本发明提供了编码其突变体的番茄胸苷激酶的新型多核苷酸，包含多核苷酸的载体构建体，携带多核苷酸或载体的宿主细胞，使前体药物致敏细胞的方法，在温血动物中抑制病原体的方法 用于植物的生物防治，合成单磷酸盐的方法和包含本发明的植物胸苷激酶的药物组合物。 在优选的实施方案中，本发明提供植物胸苷激酶和核苷类似物核苷类似物AZT（3'-叠氮基-3'-脱氧胸苷）的独特组合来治疗异常细胞生长。
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公开(公告)号：US20060035850A1
公开(公告)日：2006-02-16
申请号：US10515417
申请日：2003-05-21
CPC分类号： C12N15/8261 , A61K31/7072 , A61K38/45 , A61K45/06 , C12N9/1205 , C12N9/1211 , C12Y207/01076 , Y02A40/146
摘要： This invention relates to novel plant thymidine kinases and their use in gene therapy. More specifically the invention provides novel thymidine kinases derived from tomato, pine, rice or thale cress. In further aspects the invention provides novel polynucleotides encoding the plant thymidine kinases, vector constructs comprising the polynucleotide, host cells carrying the polynucleotide or vector, methods of sensitising cells to prodrugs, methods of inhibiting pathogenic agents in warm-blooded animals, methods for biocontrol of plants, methods of synthesizing monophosphates and pharmaceutical compositions comprising the plant thymidine kinases of the invention. In a preferred embodiment the invention provides a unique combination of a plant thymidine kinase and the nucleoside analog AZT to treat abnormal cell growth.
摘要翻译： 本发明涉及新型植物胸苷激酶及其在基因治疗中的应用。 更具体地，本发明提供了衍生自番茄，松树，水稻或鲟鱼水芹的新型胸苷激酶。 在另外的方面，本发明提供了编码植物胸苷激酶的新型多核苷酸，包含多核苷酸的载体构建体，携带多核苷酸或载体的宿主细胞，使前体药物致敏细胞的方法，温血动物中抑制病原体的方法，生物防治方法 植物，合成单磷酸盐的方法和包含本发明的植物胸苷激酶的药物组合物。 在优选的实施方案中，本发明提供植物胸苷激酶和核苷类似物AZT的独特组合以治疗异常细胞生长。
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