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公开(公告)号:US5814502A
公开(公告)日:1998-09-29
申请号:US613997
申请日:1996-03-11
CPC分类号: C12N9/96 , C12N9/12 , C12N9/1241 , C12Q1/68 , C12Q1/6816 , C12Q1/6846
摘要: Stable enzyme compositions are provided in a liquid form for the enzymatic radioactive and non-radioactive labelling of nucleic acids and oligonucleotides. The mixtures preferably contain 30-60% (v/v) glycerin. At a storage temperature between -20 .degree. C. and +4 .degree. C. over a period of at least 12 months, the mixtures do not exhibit any loss of activity.
摘要翻译: 以液体形式提供稳定的酶组合物用于核酸和寡核苷酸的酶放射性和非放射性标记。 混合物优选含有30-60%(v / v)甘油。 在-20℃至+4℃的储存温度下至少12个月的时间内,混合物不会表现出任何的活性损失。
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公开(公告)号:US4746609A
公开(公告)日:1988-05-24
申请号:US742800
申请日:1985-06-10
申请人: Bryan Bolton , Michael J. Comer , Christoph Kessler , Georg Nesch
发明人: Bryan Bolton , Michael J. Comer , Christoph Kessler , Georg Nesch
CPC分类号: C12N9/22 , Y10S435/824
摘要: The present invention teaches restriction endonuclease Asp 718 which cleaves DNA in the palindromic recognition sequence at the sites indicated by the arrow: ##STR1## The endonuclease, obtained from Achromobacter species 718, DSM 2969, is useful in obtaining DNA fragments, e.g., for determining nucleotide sequences and other types of analysis of DNA.
摘要翻译: 本发明教导了限制性内切核酸酶Asp 718,其在由箭头指示的位点处的回文识别序列中切割DNA:从无色杆菌属物种718(DSM 2969)获得的核酸内切酶可用于获得DNA片段,例如用于确定 核苷酸序列和其他类型的DNA分析。
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