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    Separation method and device for protein mixtures
    1.
    发明申请
    Separation method and device for protein mixtures 失效
    蛋白质混合物的分离方法和装置

    公开(公告)号：US20060000712A1

    公开(公告)日：2006-01-05

    申请号：US11120098

    申请日：2005-05-02

    申请人： Hans-Joachim Hoeltke ,  Albert Roeder ,  Alois Rainer ,  Peter Berndt ,  Carlo Effenhauser ,  Hanno Langen ,  Remo Hochstrasser

    发明人： Hans-Joachim Hoeltke ,  Albert Roeder ,  Alois Rainer ,  Peter Berndt ,  Carlo Effenhauser ,  Hanno Langen ,  Remo Hochstrasser

    IPC分类号： B01D57/02 ,  C02F1/40

    CPC分类号： G01N27/44773

    摘要： A separation device and a separation method for biomolecular sample material and in particular protein mixtures. For this purpose a separation element 10 for the two-dimensional and preferable electrophoretic separation of components of the sample material is provided in area 30 of a separation plane. According to the invention it is proposed that the separation element 10 has a channel or transfer structure 14 for the locally resolved discharge of separated sample components in a transport direction that is at right angles to the separation plane onto a support surface 16 that is preferably suitable for mass spectroscopic analyses.

    摘要翻译： 用于生物分子样品材料，特别是蛋白质混合物的分离装置和分离方法。 为此，在分离平面的区域30中提供用于样品材料的组分的二维和优选的电泳分离的分离元件10。 根据本发明，提出了分离元件10具有通道或转移结构14，用于将分离的样品组分在与分离平面成直角的运输方向上局部分解排放到支撑表面16上，其优选地适合 用于质谱分析。

    Separation method and device for protein mixtures
    2.
    发明授权
    Separation method and device for protein mixtures 失效
    蛋白质混合物的分离方法和装置

    公开(公告)号：US07645369B2

    公开(公告)日：2010-01-12

    申请号：US11120098

    申请日：2005-05-02

    申请人： Hans-Joachim Hoeltke ,  Albert Roeder ,  Alois Rainer ,  Peter Berndt ,  Carlo Effenhauser ,  Hanno Langen ,  Remo Anton Hochstrasser

    发明人： Hans-Joachim Hoeltke ,  Albert Roeder ,  Alois Rainer ,  Peter Berndt ,  Carlo Effenhauser ,  Hanno Langen ,  Remo Anton Hochstrasser

    IPC分类号： G01N27/447 ,  G01N27/453

    CPC分类号： G01N27/44773

    摘要： A separation device and a separation method for biomolecular sample material and in particular protein mixtures. For this purpose a separation element 10 for the two-dimensional and preferable electrophoretic separation of components of the sample material is provided in area 30 of a separation plane. According to the invention it is proposed that the separation element 10 has a channel or transfer structure 14 for the locally resolved discharge of separated sample components in a transport direction that is at right angles to the separation plane onto a support surface 16 that is preferably suitable for mass spectroscopic analyses.

    摘要翻译： 用于生物分子样品材料，特别是蛋白质混合物的分离装置和分离方法。 为此，在分离平面的区域30中提供用于样品材料的组分的二维和优选的电泳分离的分离元件10。 根据本发明，提出了分离元件10具有通道或转移结构14，用于将分离的样品组分在与分离平面成直角的运输方向上局部分解排放到支撑表面16上，其优选地适合 用于质谱分析。

    Nucleotides labelled with an infrared dye and their use in nucleic acid detection
    3.
    发明授权
    Nucleotides labelled with an infrared dye and their use in nucleic acid detection 失效
    用红外染料标记的核苷酸及其在核酸检测中的用途

    公开(公告)号：US06573374B1

    公开(公告)日：2003-06-03

    申请号：US08411761

    申请日：1995-03-28

    申请人： Klaus Muehlegger ,  Hans-Joachim Hoeltke ,  Christian Birkner ,  Herbert Von Der Eltz

    发明人： Klaus Muehlegger ,  Hans-Joachim Hoeltke ,  Christian Birkner ,  Herbert Von Der Eltz

    IPC分类号： C07H1904

    CPC分类号： C07H19/10 ,  C07F9/5728 ,  C07H19/20 ,  C07H21/00

    摘要： Nucleoside-5′-triphosphates and phosphoramidites which carry a residue absorbing in the long wavelength region, preferably a carbocyanine group of the general formula (I), on the base portion or on the phosphorus atom in which R1 and R2 each denote hydrogen or together form a phenyl residue; R3 denotes hydrogen if linkage with the nucleotide is via the R4 position or it denotes a —NHCS— group if linkage with the nucleotide is via the R3 position; both R4 and R5, or R5, alone denote an alkylsulfonyl group with n being a number from 3 to 5 or R4 represents a —NHCS— group with n being a number from 3 to 8, as well as the use of the compounds to label, detect and sequence nucleic acids.

    摘要翻译： 核苷-5'-三磷酸和亚磷酰胺，其在碱性部分或磷原子上具有在长波长区域吸收的残基，优选为通式（I）的碳菁基团，其中R1和R2各自表示氢或一起形成 苯基残基; 如果与核苷酸的连接通过R4位置，R3表示氢，如果与核苷酸的连接通过R3位置，则表示-NHCS-基团; R4和R5或R5独立地表示烷基磺酰基，n为3至5的数，或R4表示-NHCS-基，n为3至8的数，以及该化合物用于标记 ，检测和序列核酸。