Method for the analytical separation of viruses
    3.
    发明授权
    Method for the analytical separation of viruses 失效
    病毒分析方法

    公开(公告)号:US5723031A

    公开(公告)日:1998-03-03

    申请号:US547765

    申请日:1995-10-24

    CPC分类号: C12Q1/70 C12Q1/04 G01N27/447

    摘要: The method permits the non-destructive analytical detection and/or the quantification of viruses or viral particles (analytes) in a liquid sample matrix containing organic or inorganic minor constituents, in particular protein moieties and/or nucleotides and/or other viruses. To this end the viruses or the viral particles are separated from the protein moieties and/or nucleotides in the sample matrix using capillary electrophoresis and at the same time the electropherogram associated therewith is recorded. The fractions assigned to the analytes can then be identified as virus peaks in the electropherogram by spectroscopic interpretation with the aid of characteristic maxima. For better identification, a reference sample of a known virus or of a known viral particle can be added to the sample matrix.

    摘要翻译: 该方法允许在含有机或无机次要成分,特别是蛋白质部分和/或核苷酸和/或其他病毒的液体样品基质中对病毒或病毒颗粒(分析物)进行非破坏性分析检测和/或定量。 为此,使用毛细管电泳将病毒或病毒颗粒与样品基质中的蛋白质部分和/或核苷酸分开,同时记录与其相关的电泳图。 分配到分析物的级分可以通过特征最大值的光谱解释识别为电泳图中的病毒峰。 为了更好地鉴定,可以将已知病毒或已知病毒颗粒的参考样品加入到样品基质中。