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    Polypeptide Having Rnase III Activity
    5.
    发明申请
    Polypeptide Having Rnase III Activity 审中-公开
    具有Rnase III活性的多肽

    公开(公告)号：US20070218524A1

    公开(公告)日：2007-09-20

    申请号：US10573381

    申请日：2004-09-29

    申请人： Jun Tomono ,  Harumi Ueno ,  Hiroaki Sagawa ,  Ikunoshin Kato

    发明人： Jun Tomono ,  Harumi Ueno ,  Hiroaki Sagawa ,  Ikunoshin Kato

    IPC分类号： C12P21/00 ,  C07H21/02 ,  C07K2/00

    CPC分类号： C12N15/111 ,  C12N9/22 ,  C12N2310/14 ,  C12N2330/30 ,  C12Y301/26003

    摘要： A polypeptide having an RNase III activity with which the length of a dsRNA degradation product can be easily controlled depending on reaction conditions and, in preparing a dsRNA having a length allowing it to serve as an siRNA in RNA interference, a low-molecular weight product having little RNA interferring effect is scarcely formed; a method of degrading a dsRNA with the use of the above polypeptide; and a composition and a kit for the above method.

    摘要翻译： 可以根据反应条件容易地控制dsRNA降解产物的长度的具有RNase III活性的多肽，并且在制备具有允许其在RNA干扰中作为siRNA的长度的dsRNA时，低分子量产物 几乎没有形成RNA干扰效果; 使用上述多肽降解dsRNA的方法; 以及用于上述方法的组合物和试剂盒。

    Methods of degrading dsrna and synthesizing rna
    6.
    发明申请
    Methods of degrading dsrna and synthesizing rna 审中-公开
    降解dsrna并合成rna的方法

    公开(公告)号：US20070105113A1

    公开(公告)日：2007-05-10

    申请号：US10567731

    申请日：2004-08-10

    申请人： Hiroaki Sagawa ,  Jun Tomono ,  Harumi Ueno ,  Ikunoshin Kato

    发明人： Hiroaki Sagawa ,  Jun Tomono ,  Harumi Ueno ,  Ikunoshin Kato

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N9/22

    CPC分类号： C12N9/22 ,  C12P19/34

    摘要： A protein having an activity of degrading a dsRNA, namely, being capable of acting on a long-chain dsRNA to form a dsRNA of a definite length; a method of efficiently preparing a dsRNA of a definite length which comprises treating a dsRNA with the protein having an activity of degrading a dsRNA in the coexistence of a protein having an activity of binding to a nucleic acid such as a protein having an RNA-binding activity; and a method of using the protein having an activity of binding to a nucleic acid to elevate the efficiency in an RNA synthesis reaction typified by dsRNA synthesis.

    摘要翻译： 具有降解dsRNA活性的蛋白质，即能够作用于长链dsRNA以形成一定长度的dsRNA; 一种有效制备一定长度的dsRNA的方法，该方法包括在具有与核酸如具有RNA结合性的蛋白质结合的蛋白质共存的蛋白质共同存在下具有降解dsRNA活性的蛋白质对dsRNA进行处理 活动; 以及使用具有与核酸结合的活性的蛋白质以提高以dsRNA合成为代表的RNA合成反应中的效率的方法。