公开(公告)号：US06737024B1

公开(公告)日：2004-05-18

申请号：US09214868

申请日：1999-01-14

申请人： Heinz Eipel ,  Harald Keller

发明人： Heinz Eipel ,  Harald Keller

IPC分类号： G01N3348

CPC分类号： G01N33/543 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00527 ,  C40B60/14

摘要： The invention relates to solid supports for analytical measurement methods which are essentially composed of an inert solid support material on which hydrophilic measurement zones, separated from one another by a hydrophobic coating, where at least 10 measurement points are applied to the support per cm2. The invention furthermore relates to a process for producing the supports, and to the use of the supports in diagnostic methods, in research looking for active substances, in combinatorial chemistry, in crop protection, in toxicology or in environmental protection.

摘要翻译： 本发明涉及用于分析测量方法的固体支持物，其基本上由惰性固体支持材料组成，其上亲水测量区域通过疏水涂层彼此分离，其中至少10个测量点施加到每cm 2的载体上 >。 本发明还涉及用于制备载体的方法，以及在诊断方法中使用载体，研究寻找活性物质，组合化学，作物保护，毒理学或环境保护。

公开(公告)号：US08212866B2

公开(公告)日：2012-07-03

申请号：US12404488

申请日：2009-03-16

申请人： Paul Lemmer ,  Christoph Cremer ,  David Baddeley ,  Heinz Eipel

发明人： Paul Lemmer ,  Christoph Cremer ,  David Baddeley ,  Heinz Eipel

IPC分类号： H04N7/18

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N2021/6441 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/367 ,  G02B27/58

摘要： A method and apparatus are provided for obtaining a sub-resolution spatial information of a sample labeled with at least one type fluorescent label. The sub-resolution spatial information has localization information about the positions of fluorescent molecules of the at least one type fluorescent label in at least one spatial direction. The method acquires localization image data by employing fluorescence localization microscopy. The acquired localization image data is processed to obtain the localization information about the positions of fluorescent molecules of the at least one type fluorescent label in at least one spatial direction. The step of processing includes determining in each of the detected images of the series the positions of the barycenters of the detected fluorescence emission distributions from the single fluorescent molecules of the one or more fluorescent labels in at least one spatial direction.

摘要翻译： 提供一种方法和装置，用于获得用至少一种类型的荧光标签标记的样品的亚分辨率空间信息。 子分辨率空间信息具有关于至少一个类型的荧光标记在至少一个空间方向上的荧光分子的位置的定位信息。 该方法采用荧光定位显微镜获取定位图像数据。 处理获取的定位图像数据以获得关于至少一个类型的荧光标记物的荧光分子在至少一个空间方向上的位置的定位信息。 处理步骤包括在至少一个空间方向上确定该系列的每个检测到的图像中的一个或多个荧光标记的单个荧光分子的检测的荧光发射分布的重心的位置。

公开(公告)号：US08174761B2

公开(公告)日：2012-05-08

申请号：US12481927

申请日：2009-06-10

申请人： Roman Amberger ,  Christoph Cremer ,  Heinz Eipel

发明人： Roman Amberger ,  Christoph Cremer ,  Heinz Eipel

IPC分类号： G02B21/00

CPC分类号： G02B21/06 ,  G02B21/16 ,  G02B21/18 ,  G02B21/365

摘要： A total internal reflection microscope for epi-fluorescence illumination observations includes an objective through which an object to be observed is illuminated by an excitation illumination light at an angle to an observation axis of the microscope. The angle is adjustable to be within the range suitable for a total internal reflection observation. The microscope also has a source of collimated excitation light. An interferometer is arranged in the optical path of the collimated excitation light and is configured to produce an interference pattern. A focusing lens system focuses the interference pattern produced by the interferometer into the back focal plane of the objective. The objective and the focusing lens system image the interference pattern produced by the interferometer into the conjugated image plane of the objective, thereby producing excitation illumination light that modulated spatially in intensity in a plane orthogonal to the observation axis of the microscope.

摘要翻译： 用于表面荧光照明观察的全内反射显微镜包括物镜，通过该物镜通过与显微镜的观察轴成一定角度的激发照明光照射被观察物体。 该角度可调整在适合于全内反射观察的范围内。 显微镜还具有准直激发光源。 干涉仪布置在准直激发光的光路中并且被配置为产生干涉图案。 聚焦透镜系统将由干涉仪产生的干涉图案聚焦到物镜的后焦平面中。 目标和聚焦透镜系统将由干涉仪产生的干涉图案成像到目标的共轭像平面中，从而产生在垂直于显微镜的观察轴的平面中在空间上调制的激发照明光。

公开(公告)号：US06599755B1

公开(公告)日：2003-07-29

申请号：US09959379

申请日：2001-10-24

申请人： Heinz Eipel ,  Stefan Emig ,  Siegfried Hauer ,  Walter Weishaar

发明人： Heinz Eipel ,  Stefan Emig ,  Siegfried Hauer ,  Walter Weishaar

IPC分类号： B01L302

CPC分类号： B01L3/0268 ,  B01J2219/00364 ,  B01J2219/00605 ,  B01J2219/00657 ,  B41J2/175 ,  C40B60/14 ,  Y10T436/2575

摘要： The present invention relates to a method of depositing small amounts of liquid on a substrate (13) by using a pipette tip (1), which is connected to a flexible delivery line (2). Liquid is taken up by expanding the volume in the delivery line (2), and liquid is ejected by exposing the line to an impulse that is transmitted to the liquid contained in it.

摘要翻译： 本发明涉及一种通过使用连接到柔性输送管线（2）的移液管末端（1）在基底（13）上沉积少量液体的方法。 通过扩大输送管线（2）中的体积来吸收液体，并且通过将管线暴露于其中包含的液体的脉冲而喷射液体。

公开(公告)号：US06234033B1

公开(公告)日：2001-05-22

申请号：US09301578

申请日：1999-04-29

申请人： Heinz Eipel

发明人： Heinz Eipel

IPC分类号： G01N3510

CPC分类号： G01N35/1011 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00364 ,  B01J2219/00691 ,  C40B60/14 ,  G01N35/109 ,  G01N2035/0491

摘要： The invention relates to an automatic pipetting apparatus, having a drive for moving a pipetting instrument from a source site to a target site, in which apparatus end stops (8-10, 14, 15), which can be finely adjusted automatically, for the movements of the pipetting instrument (1) generated by the drive are provided at the source site and/or the target site.

摘要翻译： 本发明涉及一种自动移液装置，其具有用于将移液器从源部位移动到目标部位的驱动装置，其中装置端部停止（8-10,14,15），其可以自动精细调节，用于 由驱动器产生的移液器（1）的动作设置在源部位和/或目标部位。

公开(公告)号：US20090237501A1

公开(公告)日：2009-09-24

申请号：US12404488

申请日：2009-03-16

申请人： Paul Lemmer ,  Christoph Cremer ,  David Baddeley ,  Heinz Eipel

发明人： Paul Lemmer ,  Christoph Cremer ,  David Baddeley ,  Heinz Eipel

IPC分类号： H04N7/18

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N2021/6441 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/367 ,  G02B27/58

摘要： A method and apparatus are provided for obtaining a sub-resolution spatial information of a sample labeled with at least one type fluorescent label. The sub-resolution spatial information has localization information about the positions of fluorescent molecules of the at least one type fluorescent label in at least one spatial direction. The method acquires localization image data by employing fluorescence localization microscopy. The acquired localization image data is processed to obtain the localization information about the positions of fluorescent molecules of the at least one type fluorescent label in at least one spatial direction. The step of processing includes determining in each of the detected images of the series the positions of the barycenters of the detected fluorescence emission distributions from the single fluorescent molecules of the one or more fluorescent labels in at least one spatial direction.

摘要翻译： 提供一种方法和装置，用于获得用至少一种类型的荧光标签标记的样品的亚分辨率空间信息。 子分辨率空间信息具有关于至少一个类型的荧光标记在至少一个空间方向上的荧光分子的位置的定位信息。 该方法采用荧光定位显微镜获取定位图像数据。 处理获取的定位图像数据以获得关于至少一个类型的荧光标记物的荧光分子在至少一个空间方向上的位置的定位信息。 处理步骤包括在至少一个空间方向上确定该系列的每个检测到的图像中的一个或多个荧光标记的单个荧光分子的检测的荧光发射分布的重心的位置。

公开(公告)号：US20070059217A1

公开(公告)日：2007-03-15

申请号：US11556650

申请日：2006-11-03

申请人： Heinz Eipel ,  Markus Beier ,  Stefan Matysiak

发明人： Heinz Eipel ,  Markus Beier ,  Stefan Matysiak

IPC分类号： B01L3/02

CPC分类号： B01L3/0241 ,  G01N2035/1025 ,  G01N2035/1034 ,  Y10T436/2575

摘要： The invention relates to a process and a device for the determination of the transfer of a sample substance in the production of biopolymer fields or biopolymer arrays onto the surface (4) of a specimen slide (3). The surface (4) of a specimen slide (3) comprises a conductive material (14) whose electrical connection to the feed device (1) via the sample liquid (12) serves as acknowledgement signal (8).

摘要翻译： 本发明涉及用于确定生物聚合物领域或生物聚合物阵列生产中的样品物质在试样载玻片（3）的表面（4）上的转移的方法和装置。 试件滑动件（3）的表面（4）包括导电材料（14），其经由样品液体（12）与进料装置（1）的电连接用作确认信号（8）。

公开(公告)号：US20100315708A1

公开(公告)日：2010-12-16

申请号：US12481927

申请日：2009-06-10

申请人： Roman Amberger ,  Christoph Cremer ,  Heinz Eipel

发明人： Roman Amberger ,  Christoph Cremer ,  Heinz Eipel

IPC分类号： G02B21/06 ,  G02B21/36

CPC分类号： G02B21/06 ,  G02B21/16 ,  G02B21/18 ,  G02B21/365

摘要： A total internal reflection microscope for epi-fluorescence illumination observations includes an objective through which an object to be observed is illuminated by an excitation illumination light at an angle to an observation axis of the microscope. The angle is adjustable to be within the range suitable for a total internal reflection observation. The microscope also has a source of collimated excitation light. An interferometer is arranged in the optical path of the collimated excitation light and is configured to produce an interference pattern. A focusing lens system focuses the interference pattern produced by the interferometer into the back focal plane of the objective. The objective and the focusing lens system image the interference pattern produced by the interferometer into the conjugated image plane of the objective, thereby producing excitation illumination light that modulated spatially in intensity in a plane orthogonal to the observation axis of the microscope.

摘要翻译： 用于表面荧光照明观察的全内反射显微镜包括物镜，通过该物镜通过与显微镜的观察轴成一定角度的激发照明光照射被观察物体。 该角度可调整在适合于全内反射观察的范围内。 显微镜还具有准直激发光源。 干涉仪布置在准直激发光的光路中并且被配置为产生干涉图案。 聚焦透镜系统将由干涉仪产生的干涉图案聚焦到物镜的后焦平面中。 目标和聚焦透镜系统将由干涉仪产生的干涉图案成像到物镜的共轭像平面中，从而产生在与显微镜的观察轴正交的平面上的强度空间调制的激发照明光。

公开(公告)号：US4752131A

公开(公告)日：1988-06-21

申请号：US717107

申请日：1985-03-28

申请人： Josef Eisenlauer ,  Dieter Horn ,  Walter Ditter ,  Heinz Eipel

发明人： Josef Eisenlauer ,  Dieter Horn ,  Walter Ditter ,  Heinz Eipel

IPC分类号： G01N21/53 ,  C02F1/52 ,  G01N15/00 ,  G01N15/02 ,  G01N15/14

CPC分类号： G01N15/1459 ,  C02F1/5209 ,  G01N2015/0092 ,  G01N2015/0222 ,  G01N2015/1409 ,  G01N21/53 ,  G01N2201/08

摘要： In a laser-optical arrangement for producing signals by light scattering, obscuration or reflection, from flowing particles and/or groups of particles, which are recorded, as time-dependent voltage/current fluctuations, by means of a photodetector (9) via appropriate imaging optics (7,8,18), assignment to the degree of dispersion of the flowing system is based on a subsequent measurement (10) of the root-mean-square value, and, according to the invention, the sample stream (1) is separated from a transmitter (6,7,18) and a receiver (8,9,10) by an enveloping stream (4), and the transmitter side consists of an individual light-transmitting fiber (7) provided with a means for parallel or focused emergence of a light bundle having a diameter of the order of magnitude of the particles and/or groups of particles.

摘要翻译： 在用于通过适当的光电检测器（9）作为时间相关的电压/电流波动记录的流动的颗粒和/或颗粒组的光散射，遮蔽或反射产生信号的激光 - 光学装置中， 成像光学器件（7,8,18），对流动系统的分散程度的分配基于随机的均方根值（10），并且根据本发明，样品流（1 ）通过包络流（4）与发射机（6,7,18）和接收机（8,9,10）分离，并且发射机侧由单独的光传输光纤（7）组成，其具有装置 用于平行或集中地出现具有颗粒和/或颗粒组的数量级的直径的光束。