公开(公告)号：US20060211005A1

公开(公告)日：2006-09-21

申请号：US11180881

申请日：2005-07-14

申请人： Hidenobu Yaku ,  Masayoshi Maeshima ,  Yoichi Nakanishi ,  Megumi Hirono ,  Tetsuo Yukimasa ,  Hiroaki Oka

发明人： Hidenobu Yaku ,  Masayoshi Maeshima ,  Yoichi Nakanishi ,  Megumi Hirono ,  Tetsuo Yukimasa ,  Hiroaki Oka

IPC分类号： C12Q1/68 ,  C12Q1/34

CPC分类号： B82Y15/00 ,  B82Y30/00 ,  C12Q1/42 ,  C12Q1/6846 ,  G01N33/56911 ,  C12Q2565/301

摘要： An object of the present invention is to provide a method of quantitative determination of pyrophosphate (PPi) and a method of detecting a primer extension reaction that require fewer kinds of enzymes and that do not necessitate strict temperature regulation, and a kit and a device for performing these methods. In the method of measuring PPi of the present invention, a PPi sample having an unknown concentration is allowed to act on mycobacterial H+-pyrophosphatase (H+-PPase) intrinsically included in membrane, thereby measuring the PPi concentration in the sample by analyzing thus resulting H+transport. Furthermore, in the method of detecting an extension reaction of a primer of the present invention, an unknown nucleic acid sample to which examination of occurrence of the primer extension reaction is intended is allowed to act on mycobacterial H+-PPase intrinsically included in a membrane, thereby determining occurrence of the primer extension reaction on the unknown nucleic acid sample by analyzing thus resulting H+transport.

摘要翻译： 本发明的目的是提供一种定量测定焦磷酸盐（PPi）的方法和检测需要更少种类的酶并且不需要严格的温度调节的引物延伸反应的方法，以及用于 执行这些方法。 在本发明的PPi测定方法中，使具有未知浓度的PPi试样作用于本质上包含的分枝杆菌H + + - 磷酸酶（H + + - 磷酸酶） 从而通过分析由此得到的H +运输来测量样品中的PPi浓度。 此外，在检测本发明的引物的延伸反应的方法中，允许对引物延伸反应的出现进行检查的未知核酸样品作用于分枝杆菌H + - 本质上包含在膜中，从而通过分析由此产生的H +运输来确定未知核酸样品上的引物延伸反应的发生。

公开(公告)号：US07396648B2

公开(公告)日：2008-07-08

申请号：US11607907

申请日：2006-12-04

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6827 ,  Y10T436/143333

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of T or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 一个基座间隔开的是G.根据本发明，3'端基被设计为对应于SNP的基极; 从3'端的第二个基地是T或G; 从3'的第三个基地是T或G的任何一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

公开(公告)号：US07348150B1

公开(公告)日：2008-03-25

申请号：US11633587

申请日：2006-12-05

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6851 ,  C12Q2565/301

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of A, T or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

公开(公告)号：US20070281341A1

公开(公告)日：2007-12-06

申请号：US11633584

申请日：2006-12-05

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12P19/34

CPC分类号： C12Q1/6858 ,  C12Q1/6869 ,  C12Q2535/125

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is C.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of A, T or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 其中一个基座间隔开C.根据本发明，3'端基被设计为对应于SNP的基底; 从3'端的第二个基地是T或G; 3'端的第3个碱基为A，T或C之一; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

公开(公告)号：US07297498B2

公开(公告)日：2007-11-20

申请号：US11488635

申请日：2006-07-19

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6858 ,  C12Q2535/125 ,  C12Q2525/185

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is C.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second and the third bases from the 3′ end to be 5′-AT-3′, 5′-TA-3′, 5′-TT-3′ or 5′-CT-3′; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基是C时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 其中一个基座间隔开C.根据本发明，3'端基被设计为对应于SNP的基底; 3'末端的第2和第3个碱基为5'-AT-3'，5'-TA-3'，5'-TT-3'或5'-CT-3' 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

公开(公告)号：US07285387B2

公开(公告)日：2007-10-23

申请号：US11363953

申请日：2006-03-01

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6858 ,  C12Q2535/125 ,  C12Q2525/185

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is A, while a base adjacent with one base spaced apart is A. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be C; the third base from the 3′ end to be any one of A or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当靶SNP碱基的3'侧邻近的碱基为A时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 一个分隔开的基底是A.根据本发明，3'端基被设计为对应于SNP的碱基; 从3'的第二个底部到C; 3'端的第3个碱基为A或C的任何一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

公开(公告)号：US20050158725A1

公开(公告)日：2005-07-21

申请号：US10510819

申请日：2003-09-24

申请人： Tetsuo Yukimasa ,  Nobuhiko Ozaki ,  Masaya Nakatani ,  Hidenobu Yaku ,  Hiroaki Oka ,  Kimi Fukahi

发明人： Tetsuo Yukimasa ,  Nobuhiko Ozaki ,  Masaya Nakatani ,  Hidenobu Yaku ,  Hiroaki Oka ,  Kimi Fukahi

IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12M1/34

CPC分类号： C07H21/00 ,  C12Q1/6844

摘要： The present invention provides a reaction container, a reaction device, and a method of amplifying a nucleic acid, which are suitable for locally heating a sample which contains a nucleic acid, enable rapid control of temperature changes in the sample, provide easy fabrication, and are low cost. The invention provides a reaction container used for a nucleic acid amplification reaction, which includes a sample holding portion formed from a cavity in the reaction container; and a heating element for heating a sample which contains a nucleic acid and which is held in the sample holding portion, wherein the heating element is made of a conductive material and arranged at a location where the sample or the sample and the periphery of the cavity is (are) to be locally heated, the conductive material generating heat by electromagnetic induction.

摘要翻译： 本发明提供一种反应容器，反应装置和扩增核酸的方法，其适用于局部加热含有核酸的样品，能够快速控制样品中的温度变化，提供容易的制造，以及 是低成本的。 本发明提供了用于核酸扩增反应的反应容器，其包括由反应容器中的空腔形成的样品保持部分; 以及加热元件，用于加热含有核酸并被保持在样品保持部分中的样品，其中所述加热元件由导电材料制成并且布置在样品或样品和空腔周边的位置 被局部加热，导电材料通过电磁感应产生热量。

公开(公告)号：US07297499B2

公开(公告)日：2007-11-20

申请号：US11489582

申请日：2006-07-20

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6858 ,  C12Q2535/125 ,  C12Q2525/185

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is C, while a base adjacent with one base spaced apart is T.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T; the third base from the 3′ end to be any one of T, G or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基是C时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 其中一个基座间隔开。根据本发明，3'端基被设计为对应于SNP的基座; 从3'端的第二个基地是T; 3'末端的第3个碱基为T，G或C任一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

公开(公告)号：US20070082344A1

公开(公告)日：2007-04-12

申请号：US11363953

申请日：2006-03-01

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6858 ,  C12Q2535/125 ,  C12Q2525/185

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is A, while a base adjacent with one base spaced apart is A. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be C; the third base from the 3′ end to be any one of A or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

公开(公告)号：US07351539B2

公开(公告)日：2008-04-01

申请号：US11633584

申请日：2006-12-05

申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6858 ,  C12Q1/6869 ,  C12Q2535/125

摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of A, T or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 其中一个基座间隔开C.根据本发明，3'端基被设计为对应于SNP的基底; 从3'端的第二个基地是T或G; 3'端的第3个碱基为A，T或C之一; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。